The inner nuclear membrane protein, Banf1, has an essential role in triple negative breast cancer cell proliferation and survival
Abstract Triple negative breast cancers (TNBCs) are a heterogenous subcategory of breast cancers which have significantly worse survival outcomes compared to other breast cancer subtypes. However, limited advances have been made towards a targeted TNBC therapeutic and traditional chemotherapies rema...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-10346-x |
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| author | Maddison Rose Joshua T. Burgess Chee Man Cheong Iain Richard Amila Suraweera Mark N. Adams Pascal H. G. Duijf Kenneth J. O’Byrne Derek J. Richard Emma Bolderson |
| author_facet | Maddison Rose Joshua T. Burgess Chee Man Cheong Iain Richard Amila Suraweera Mark N. Adams Pascal H. G. Duijf Kenneth J. O’Byrne Derek J. Richard Emma Bolderson |
| author_sort | Maddison Rose |
| collection | DOAJ |
| description | Abstract Triple negative breast cancers (TNBCs) are a heterogenous subcategory of breast cancers which have significantly worse survival outcomes compared to other breast cancer subtypes. However, limited advances have been made towards a targeted TNBC therapeutic and traditional chemotherapies remain the frontline therapy. Therefore, this study aimed to examine the therapeutic potential of a novel TNBC target, Barrier-to-Autointegration Factor 1 (Banf1), including determining Banf1 expression and cellular localisation in non-malignant breast cells and a panel of TNBC cell lines. Bioinformatic analysis of patient samples demonstrated that Banf1 is overexpressed in all breast cancer stages and subtypes. Banf1 depletion inhibited proliferation and induced mitotic arrest in TNBC cells via loss of nuclear envelope integrity and aberrant nuclear morphology, inducing TNBC tumour cell-specific cell death. These findings highlight the significant overexpression and functional involvement of Banf1 in TNBC progression and suggests that it may have potential as a novel anti-cancer target, supporting further investigation. |
| format | Article |
| id | doaj-art-6bbb1b23c0bd4f28b68f59dbc1d3ef1d |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-6bbb1b23c0bd4f28b68f59dbc1d3ef1d2025-08-20T03:42:36ZengNature PortfolioScientific Reports2045-23222025-07-0115111310.1038/s41598-025-10346-xThe inner nuclear membrane protein, Banf1, has an essential role in triple negative breast cancer cell proliferation and survivalMaddison Rose0Joshua T. Burgess1Chee Man Cheong2Iain Richard3Amila Suraweera4Mark N. Adams5Pascal H. G. Duijf6Kenneth J. O’Byrne7Derek J. Richard8Emma Bolderson9Cancer Genomics Program, Centre for Genomics and Personalised Health, School of Biomedical Sciences, Faculty of Health, Queensland University of TechnologyCancer Genomics Program, Centre for Genomics and Personalised Health, School of Biomedical Sciences, Faculty of Health, Queensland University of TechnologyCancer Genomics Program, Centre for Genomics and Personalised Health, School of Biomedical Sciences, Faculty of Health, Queensland University of TechnologyCancer Genomics Program, Centre for Genomics and Personalised Health, School of Biomedical Sciences, Faculty of Health, Queensland University of TechnologyCancer Genomics Program, Centre for Genomics and Personalised Health, School of Biomedical Sciences, Faculty of Health, Queensland University of TechnologyCancer Genomics Program, Centre for Genomics and Personalised Health, School of Biomedical Sciences, Faculty of Health, Queensland University of TechnologyCentre for Cancer Biology, University of South Australia & SA PathologyCancer Genomics Program, Centre for Genomics and Personalised Health, School of Biomedical Sciences, Faculty of Health, Queensland University of TechnologyCancer Genomics Program, Centre for Genomics and Personalised Health, School of Biomedical Sciences, Faculty of Health, Queensland University of TechnologyCancer Genomics Program, Centre for Genomics and Personalised Health, School of Biomedical Sciences, Faculty of Health, Queensland University of TechnologyAbstract Triple negative breast cancers (TNBCs) are a heterogenous subcategory of breast cancers which have significantly worse survival outcomes compared to other breast cancer subtypes. However, limited advances have been made towards a targeted TNBC therapeutic and traditional chemotherapies remain the frontline therapy. Therefore, this study aimed to examine the therapeutic potential of a novel TNBC target, Barrier-to-Autointegration Factor 1 (Banf1), including determining Banf1 expression and cellular localisation in non-malignant breast cells and a panel of TNBC cell lines. Bioinformatic analysis of patient samples demonstrated that Banf1 is overexpressed in all breast cancer stages and subtypes. Banf1 depletion inhibited proliferation and induced mitotic arrest in TNBC cells via loss of nuclear envelope integrity and aberrant nuclear morphology, inducing TNBC tumour cell-specific cell death. These findings highlight the significant overexpression and functional involvement of Banf1 in TNBC progression and suggests that it may have potential as a novel anti-cancer target, supporting further investigation.https://doi.org/10.1038/s41598-025-10346-xBreast cancerNuclear envelopeBanf1Mitosis |
| spellingShingle | Maddison Rose Joshua T. Burgess Chee Man Cheong Iain Richard Amila Suraweera Mark N. Adams Pascal H. G. Duijf Kenneth J. O’Byrne Derek J. Richard Emma Bolderson The inner nuclear membrane protein, Banf1, has an essential role in triple negative breast cancer cell proliferation and survival Scientific Reports Breast cancer Nuclear envelope Banf1 Mitosis |
| title | The inner nuclear membrane protein, Banf1, has an essential role in triple negative breast cancer cell proliferation and survival |
| title_full | The inner nuclear membrane protein, Banf1, has an essential role in triple negative breast cancer cell proliferation and survival |
| title_fullStr | The inner nuclear membrane protein, Banf1, has an essential role in triple negative breast cancer cell proliferation and survival |
| title_full_unstemmed | The inner nuclear membrane protein, Banf1, has an essential role in triple negative breast cancer cell proliferation and survival |
| title_short | The inner nuclear membrane protein, Banf1, has an essential role in triple negative breast cancer cell proliferation and survival |
| title_sort | inner nuclear membrane protein banf1 has an essential role in triple negative breast cancer cell proliferation and survival |
| topic | Breast cancer Nuclear envelope Banf1 Mitosis |
| url | https://doi.org/10.1038/s41598-025-10346-x |
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