MicroRNA-22 inhibits proliferation and promotes differentiation of satellite cells in porcine skeletal muscle
Pig is an important economic animal in China. Improving meat quality and meat productivity is a long time issue in animal genetic breeding. MicroRNAs (miRNAs) are short non-coding RNAs that participate in various biological processes, such as muscle development and embryogenesis. miR-22 differential...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2020-01-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311919627012 |
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| Summary: | Pig is an important economic animal in China. Improving meat quality and meat productivity is a long time issue in animal genetic breeding. MicroRNAs (miRNAs) are short non-coding RNAs that participate in various biological processes, such as muscle development and embryogenesis. miR-22 differentially expresses in embryonic and adult skeletal muscle. However, the underlying mechanism is unclear. In this study, we investigated miR-22 function in proliferation and differentiation of porcine satellite cells (PSCs) in skeletal muscle. Our data show that miR-22 expressed in both proliferation and differentiated PSCs and is significantly upregulated (P<0.05) during differentiation. After treated with the miR-22 inhibitor, PSCs proliferation was significantly increased (P<0.05), as indicated by the up-regulation (P<0.01) of cyclin D1 (CCND1), cyclin B1 (CCNB1) and down-regulation (P<0.05) of P21. Conversely, over-expression of miR-22 resulted in opposite results. Differentiation of PSCs was significantly suppressed (P<0.05), evidenced by two major myogenic markers: myogenin (MyoG) and myosin heavy chain (MyHC), after transfecting the PSCs with miR-22 inhibitor. Opposite results were demonstrated in the other way around by transfection with miR-22 mimics. In conclusion, the data from this study indicated that miR-22 inhibited the PSCs proliferation but promoted their differentiation. |
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| ISSN: | 2095-3119 |