Straightforward Procedure for Laboratory Production of DNA Ladder
DNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure to prepare a DNA ladder that consists of 10 fragments from 100 to 1000 bp. This protocol is a combination of routinely employed me...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.1155/2012/254630 |
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| author | Vo Thi Thuong Lan Pham Thi Thanh Loan Pham Anh Thuy Duong Le Thi Thanh Ngo Thi Ha Ta Bich Thuan |
| author_facet | Vo Thi Thuong Lan Pham Thi Thanh Loan Pham Anh Thuy Duong Le Thi Thanh Ngo Thi Ha Ta Bich Thuan |
| author_sort | Vo Thi Thuong Lan |
| collection | DOAJ |
| description | DNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure to prepare a DNA ladder that consists of 10 fragments from 100 to 1000 bp. This protocol is a combination of routinely employed methods: cloning, PCR, and partial digestion with restriction enzymes. DNA fragments of 100 bp with unique restriction site at both ends were self-ligated to create a tandem repeat. Once being cloned, the tandem repeat was rapidly amplified by PCR and partially digested by restriction enzymes to produce a ladder containing multimers of the repeated DNA fragments. Our procedure for production of DNA ladder could be simple, time saving, and inexpensive in comparison with current ones widely used in most laboratories. |
| format | Article |
| id | doaj-art-6b23e3d55dd74f2e9bf3d10a62de866b |
| institution | Kabale University |
| issn | 2090-0201 2090-021X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-6b23e3d55dd74f2e9bf3d10a62de866b2025-02-03T05:47:43ZengWileyJournal of Nucleic Acids2090-02012090-021X2012-01-01201210.1155/2012/254630254630Straightforward Procedure for Laboratory Production of DNA LadderVo Thi Thuong Lan0Pham Thi Thanh Loan1Pham Anh Thuy Duong2Le Thi Thanh3Ngo Thi Ha4Ta Bich Thuan5Faculty of Biology, Hanoi University of Science, Vietnam National University, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, VietnamFaculty of Biology, Hanoi University of Science, Vietnam National University, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, VietnamFaculty of Biology, Hanoi University of Science, Vietnam National University, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, VietnamFaculty of Biology, Hanoi University of Science, Vietnam National University, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, VietnamFaculty of Biology, Hanoi University of Science, Vietnam National University, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, VietnamFaculty of Biology, Hanoi University of Science, Vietnam National University, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, VietnamDNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure to prepare a DNA ladder that consists of 10 fragments from 100 to 1000 bp. This protocol is a combination of routinely employed methods: cloning, PCR, and partial digestion with restriction enzymes. DNA fragments of 100 bp with unique restriction site at both ends were self-ligated to create a tandem repeat. Once being cloned, the tandem repeat was rapidly amplified by PCR and partially digested by restriction enzymes to produce a ladder containing multimers of the repeated DNA fragments. Our procedure for production of DNA ladder could be simple, time saving, and inexpensive in comparison with current ones widely used in most laboratories.http://dx.doi.org/10.1155/2012/254630 |
| spellingShingle | Vo Thi Thuong Lan Pham Thi Thanh Loan Pham Anh Thuy Duong Le Thi Thanh Ngo Thi Ha Ta Bich Thuan Straightforward Procedure for Laboratory Production of DNA Ladder Journal of Nucleic Acids |
| title | Straightforward Procedure for Laboratory Production of DNA Ladder |
| title_full | Straightforward Procedure for Laboratory Production of DNA Ladder |
| title_fullStr | Straightforward Procedure for Laboratory Production of DNA Ladder |
| title_full_unstemmed | Straightforward Procedure for Laboratory Production of DNA Ladder |
| title_short | Straightforward Procedure for Laboratory Production of DNA Ladder |
| title_sort | straightforward procedure for laboratory production of dna ladder |
| url | http://dx.doi.org/10.1155/2012/254630 |
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