Transcriptional changes across tissue and time provide molecular insights into a therapeutic window of opportunity following traumatic stress exposure
Abstract Unfortunately, survivors of traumatic stress exposure (TSE) frequently develop adverse posttraumatic neuropsychiatric sequelae (APNS) such as chronic pain and stress/depressive symptoms. Increasing evidence indicates that there is a ‘window of opportunity’ following TSE in which therapeutic...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2025-07-01
|
| Series: | Translational Psychiatry |
| Online Access: | https://doi.org/10.1038/s41398-025-03451-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Abstract Unfortunately, survivors of traumatic stress exposure (TSE) frequently develop adverse posttraumatic neuropsychiatric sequelae (APNS) such as chronic pain and stress/depressive symptoms. Increasing evidence indicates that there is a ‘window of opportunity’ following TSE in which therapeutic interventions are most effective against APNS, yet mechanisms accounting for this observation are poorly understood. Here, we aimed to better understand such mechanisms by generating snapshots of the transcriptional landscape in the early aftermath of TSE across tissues and time. Adult rats were exposed to a TSE model, single prolonged stress (SPS). Then, eight tissues (hypothalamus, left and right hippocampus, amygdala, dorsal root ganglia, spinal cord, heart, and muscle) were isolated from these animals at 2, 24, and 72 h after SPS and in unexposed controls (n = 6 per group). mRNA expression from deep sequencing was used to identify differentially expressed genes (DEGs), biological pathways enriched over time, and predicted upstream regulators. In all tissues except the amygdala, the highest number of DEGs was observed 2-h post-SPS, but DEGs were detected at all timepoints and in all tissues. Some transcripts were differentially expressed in a consistent manner across multiple tissues at a time point (e.g. Fkbp5, 2 h post-SPS), while others had tissue- or region-specific expression patterns. Stress system pathways were most represented at 2 h post-SPS, then stress/circadian/inflammatory pathways at 24 h, and inflammatory pathways at 72 h. Together these findings provide insights into post-TSE transcriptional landscape dynamics and suggest specific intervention windows of opportunity. Future validation is needed across sex, age, stressor, and cell type. |
|---|---|
| ISSN: | 2158-3188 |