Fel d 1 specific IgE measurement for dog exclusive owners co-sensitized to dog and cat

Background: The diagnosis of the culprit allergen depends on exposure, symptoms at exposure, and the presence of specific IgE (sIgE). Pet allergens are sticky and can sensitize individuals without adoption history. Exclusive dog owners frequently exhibit both dog (e5) and cat dander sIgE (e1). We as...

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Main Authors: Lin Liang, MD, Ah-Reum Hwang, MS, Yoon Ji Shin, BS, Kyoung Yong Jeong, PhD, Kyung Hee Park, MD, PhD, Jae-Hyun Lee, MD, PhD, Jung-Won Park, MD, PhD
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:World Allergy Organization Journal
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Online Access:http://www.sciencedirect.com/science/article/pii/S193945512400139X
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author Lin Liang, MD
Ah-Reum Hwang, MS
Yoon Ji Shin, BS
Kyoung Yong Jeong, PhD
Kyung Hee Park, MD, PhD
Jae-Hyun Lee, MD, PhD
Jung-Won Park, MD, PhD
author_facet Lin Liang, MD
Ah-Reum Hwang, MS
Yoon Ji Shin, BS
Kyoung Yong Jeong, PhD
Kyung Hee Park, MD, PhD
Jae-Hyun Lee, MD, PhD
Jung-Won Park, MD, PhD
author_sort Lin Liang, MD
collection DOAJ
description Background: The diagnosis of the culprit allergen depends on exposure, symptoms at exposure, and the presence of specific IgE (sIgE). Pet allergens are sticky and can sensitize individuals without adoption history. Exclusive dog owners frequently exhibit both dog (e5) and cat dander sIgE (e1). We assessed whether the measurement of Fel d 1 sIgE (e94) can discriminate true cat sensitization from false positivity by cross-reactivity in the exclusive dog owners. Methods: Thirty-one patients with respiratory allergies who exclusively owned dogs were enrolled for this study. e5, e1, and e94 were measured with ImmunoCAP. ELISA inhibition was performed to assess cross-reactivity. Results: About 81% of patients (25/31) were both e5 and e1 positive, and 8 were also positive for e94. In the e94 positive, cat dander exhibited higher maximum inhibition of cat sIgE (94% vs 88%) and demonstrated lower IC50 (6.5 vs 737.9 BAU/mL) compared to dog dander. Conversely, in the e94 negative, dog dander demonstrated higher maximum inhibition of cat dander sIgE (71.9% vs 56.2%) and lower IC50 (172 vs 1947 BAU/mL) compared to cat dander. In the e94 positive, dog dander exhibited higher maximal inhibition for dog sIgE (91.5 vs 76.1%) and lower IC50 (10.6 vs 1679 BAU/mL) compared to cat dander, whereas in the e94 negative, the IC50 for cat dander could not be determined. Conclusions: Genuine co-sensitization to cats is notable even in individuals who exclusively own dogs. Positive e94 results could discriminate authentic cat sensitization from false positivity by cross-reactivity in these patients, underscoring the importance of comprehensive allergy assessment.
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series World Allergy Organization Journal
spelling doaj-art-6a5aee97603947f6ae532079e4682f432024-12-13T10:57:08ZengElsevierWorld Allergy Organization Journal1939-45512024-12-011712101007Fel d 1 specific IgE measurement for dog exclusive owners co-sensitized to dog and catLin Liang, MD0Ah-Reum Hwang, MS1Yoon Ji Shin, BS2Kyoung Yong Jeong, PhD3Kyung Hee Park, MD, PhD4Jae-Hyun Lee, MD, PhD5Jung-Won Park, MD, PhD6Graduate School of Medicine, Yonsei University College of Medicine, Seoul, South Korea; Institute of Allergy, Yonsei University College of Medicine, Seoul, South KoreaInstitute of Allergy, Yonsei University College of Medicine, Seoul, South KoreaInstitute of Allergy, Yonsei University College of Medicine, Seoul, South KoreaInstitute of Allergy, Yonsei University College of Medicine, Seoul, South KoreaInstitute of Allergy, Yonsei University College of Medicine, Seoul, South Korea; Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South KoreaInstitute of Allergy, Yonsei University College of Medicine, Seoul, South Korea; Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South KoreaInstitute of Allergy, Yonsei University College of Medicine, Seoul, South Korea; Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea; Corresponding author.Background: The diagnosis of the culprit allergen depends on exposure, symptoms at exposure, and the presence of specific IgE (sIgE). Pet allergens are sticky and can sensitize individuals without adoption history. Exclusive dog owners frequently exhibit both dog (e5) and cat dander sIgE (e1). We assessed whether the measurement of Fel d 1 sIgE (e94) can discriminate true cat sensitization from false positivity by cross-reactivity in the exclusive dog owners. Methods: Thirty-one patients with respiratory allergies who exclusively owned dogs were enrolled for this study. e5, e1, and e94 were measured with ImmunoCAP. ELISA inhibition was performed to assess cross-reactivity. Results: About 81% of patients (25/31) were both e5 and e1 positive, and 8 were also positive for e94. In the e94 positive, cat dander exhibited higher maximum inhibition of cat sIgE (94% vs 88%) and demonstrated lower IC50 (6.5 vs 737.9 BAU/mL) compared to dog dander. Conversely, in the e94 negative, dog dander demonstrated higher maximum inhibition of cat dander sIgE (71.9% vs 56.2%) and lower IC50 (172 vs 1947 BAU/mL) compared to cat dander. In the e94 positive, dog dander exhibited higher maximal inhibition for dog sIgE (91.5 vs 76.1%) and lower IC50 (10.6 vs 1679 BAU/mL) compared to cat dander, whereas in the e94 negative, the IC50 for cat dander could not be determined. Conclusions: Genuine co-sensitization to cats is notable even in individuals who exclusively own dogs. Positive e94 results could discriminate authentic cat sensitization from false positivity by cross-reactivity in these patients, underscoring the importance of comprehensive allergy assessment.http://www.sciencedirect.com/science/article/pii/S193945512400139XCatCross-reactivityDogFel d 1
spellingShingle Lin Liang, MD
Ah-Reum Hwang, MS
Yoon Ji Shin, BS
Kyoung Yong Jeong, PhD
Kyung Hee Park, MD, PhD
Jae-Hyun Lee, MD, PhD
Jung-Won Park, MD, PhD
Fel d 1 specific IgE measurement for dog exclusive owners co-sensitized to dog and cat
World Allergy Organization Journal
Cat
Cross-reactivity
Dog
Fel d 1
title Fel d 1 specific IgE measurement for dog exclusive owners co-sensitized to dog and cat
title_full Fel d 1 specific IgE measurement for dog exclusive owners co-sensitized to dog and cat
title_fullStr Fel d 1 specific IgE measurement for dog exclusive owners co-sensitized to dog and cat
title_full_unstemmed Fel d 1 specific IgE measurement for dog exclusive owners co-sensitized to dog and cat
title_short Fel d 1 specific IgE measurement for dog exclusive owners co-sensitized to dog and cat
title_sort fel d 1 specific ige measurement for dog exclusive owners co sensitized to dog and cat
topic Cat
Cross-reactivity
Dog
Fel d 1
url http://www.sciencedirect.com/science/article/pii/S193945512400139X
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