FCGBP functions as a tumor suppressor gene in head and neck squamous cell carcinoma
Abstract Purpose The pathogenesis of head and neck squamous cell carcinoma (HNSCC) was complex and the overall survival was not satisfying. It was urgent to uncover novel molecules that play vital role in HNSCC for disease monitoring and drug development. Methods Distinguished expression of FCGBP mR...
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| Format: | Article |
| Language: | English |
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Springer
2024-11-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-024-01607-8 |
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| author | Lijuan Zeng Jun Zeng Jianfeng He Yongqi Li Chengwei Li Zhiyan Lin Guangwei Chen Huilin Wu Libin Zhou |
| author_facet | Lijuan Zeng Jun Zeng Jianfeng He Yongqi Li Chengwei Li Zhiyan Lin Guangwei Chen Huilin Wu Libin Zhou |
| author_sort | Lijuan Zeng |
| collection | DOAJ |
| description | Abstract Purpose The pathogenesis of head and neck squamous cell carcinoma (HNSCC) was complex and the overall survival was not satisfying. It was urgent to uncover novel molecules that play vital role in HNSCC for disease monitoring and drug development. Methods Distinguished expression of FCGBP mRNA in HNSCC was analyzed by TCGA-HNSC and three GEO datasets, the relationship between FCGBP and clinical stage and survival was analyzed by GEPIA 2, the immune infiltration pattern analysis was conducted by TIMER 2.0, pathways affected by FCGBP was conducted by GSEA and GO/KEGG. In vitro experiments (including qRT-PCR, siRNA transfection, CCK8, transwell assay and flow cytometry) were conducted to confirm bioinformatic analysis. Results FCGBP was down-regulated in tumor samples compared with normal tissues at both mRNA and protein levels, and positively correlated with survival in HNSCC. Genes co-expressed with FCGBP were mainly enriched in immune-related biological processes and pathways. GSEA indicated that FCGBP was associated with activated immune reaction and inhibiting well-known pro-tumor pathways. GSE41613 validated FCGBP as an independent prognostic marker for HNSCC and FCGBP was down-regulated in HNSCC cell lines by qRT-PCR. Migration and invasion of SCC9 and CAL27 were enhanced by FCGBP-targeting siRNAs, the ratio of cytotoxic T lymphocytes were down-regulated while the ratio of myeloid-derived suppressor cells were increased by FCGBP-targeting siRNAs. Conclusion FCGBP was a tumor suppressor gene and was an independent prognostic marker for better survival. The underlying mechanism may be that FCGBP inhibited tumor migration and invasion and activated immune response against tumor cells. |
| format | Article |
| id | doaj-art-69f10e862fd244c3b2b6ffc0f93e052d |
| institution | Kabale University |
| issn | 2730-6011 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-69f10e862fd244c3b2b6ffc0f93e052d2024-11-24T12:31:42ZengSpringerDiscover Oncology2730-60112024-11-0115111510.1007/s12672-024-01607-8FCGBP functions as a tumor suppressor gene in head and neck squamous cell carcinomaLijuan Zeng0Jun Zeng1Jianfeng He2Yongqi Li3Chengwei Li4Zhiyan Lin5Guangwei Chen6Huilin Wu7Libin Zhou8Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical UniversityGuangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative MedicineDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical UniversityDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical UniversityDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical UniversityDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical UniversityDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical UniversityDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical UniversityDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical UniversityAbstract Purpose The pathogenesis of head and neck squamous cell carcinoma (HNSCC) was complex and the overall survival was not satisfying. It was urgent to uncover novel molecules that play vital role in HNSCC for disease monitoring and drug development. Methods Distinguished expression of FCGBP mRNA in HNSCC was analyzed by TCGA-HNSC and three GEO datasets, the relationship between FCGBP and clinical stage and survival was analyzed by GEPIA 2, the immune infiltration pattern analysis was conducted by TIMER 2.0, pathways affected by FCGBP was conducted by GSEA and GO/KEGG. In vitro experiments (including qRT-PCR, siRNA transfection, CCK8, transwell assay and flow cytometry) were conducted to confirm bioinformatic analysis. Results FCGBP was down-regulated in tumor samples compared with normal tissues at both mRNA and protein levels, and positively correlated with survival in HNSCC. Genes co-expressed with FCGBP were mainly enriched in immune-related biological processes and pathways. GSEA indicated that FCGBP was associated with activated immune reaction and inhibiting well-known pro-tumor pathways. GSE41613 validated FCGBP as an independent prognostic marker for HNSCC and FCGBP was down-regulated in HNSCC cell lines by qRT-PCR. Migration and invasion of SCC9 and CAL27 were enhanced by FCGBP-targeting siRNAs, the ratio of cytotoxic T lymphocytes were down-regulated while the ratio of myeloid-derived suppressor cells were increased by FCGBP-targeting siRNAs. Conclusion FCGBP was a tumor suppressor gene and was an independent prognostic marker for better survival. The underlying mechanism may be that FCGBP inhibited tumor migration and invasion and activated immune response against tumor cells.https://doi.org/10.1007/s12672-024-01607-8HNSCCFCGBPImmune infiltrationPrognostic markerTumor suppressor gene |
| spellingShingle | Lijuan Zeng Jun Zeng Jianfeng He Yongqi Li Chengwei Li Zhiyan Lin Guangwei Chen Huilin Wu Libin Zhou FCGBP functions as a tumor suppressor gene in head and neck squamous cell carcinoma Discover Oncology HNSCC FCGBP Immune infiltration Prognostic marker Tumor suppressor gene |
| title | FCGBP functions as a tumor suppressor gene in head and neck squamous cell carcinoma |
| title_full | FCGBP functions as a tumor suppressor gene in head and neck squamous cell carcinoma |
| title_fullStr | FCGBP functions as a tumor suppressor gene in head and neck squamous cell carcinoma |
| title_full_unstemmed | FCGBP functions as a tumor suppressor gene in head and neck squamous cell carcinoma |
| title_short | FCGBP functions as a tumor suppressor gene in head and neck squamous cell carcinoma |
| title_sort | fcgbp functions as a tumor suppressor gene in head and neck squamous cell carcinoma |
| topic | HNSCC FCGBP Immune infiltration Prognostic marker Tumor suppressor gene |
| url | https://doi.org/10.1007/s12672-024-01607-8 |
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