Development a risk prediction nomogram for multidrug−resistant bacterial and fungal infection in gastrointestinal fistula patients during the perioperative period

BackgroundThis study aims to develop a risk prediction model for multidrug-resistant bacterial and fungal infections in patients with gastrointestinal fistulas during the perioperative period.MethodsA retrospective cohort study was conducted at Anhui No. 2 Provincial People’s Hospital from January 2...

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Main Authors: Mingming Yin, Haoyi Zheng, Lifeng Xu, Rong Jin, Xiangyang Wang, Yi Man, Kai Xu, Qiang Ruan, Ting Wang, Kai Guo, Zheng Zhou, Wenyong Wu, Guosheng Gu
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-11-01
Series:Frontiers in Cellular and Infection Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2024.1502529/full
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author Mingming Yin
Haoyi Zheng
Haoyi Zheng
Lifeng Xu
Rong Jin
Xiangyang Wang
Yi Man
Kai Xu
Qiang Ruan
Ting Wang
Kai Guo
Zheng Zhou
Wenyong Wu
Guosheng Gu
Guosheng Gu
author_facet Mingming Yin
Haoyi Zheng
Haoyi Zheng
Lifeng Xu
Rong Jin
Xiangyang Wang
Yi Man
Kai Xu
Qiang Ruan
Ting Wang
Kai Guo
Zheng Zhou
Wenyong Wu
Guosheng Gu
Guosheng Gu
author_sort Mingming Yin
collection DOAJ
description BackgroundThis study aims to develop a risk prediction model for multidrug-resistant bacterial and fungal infections in patients with gastrointestinal fistulas during the perioperative period.MethodsA retrospective cohort study was conducted at Anhui No. 2 Provincial People’s Hospital from January 2022 to July 2024. We analyzed the distribution, resistance patterns, and mechanisms of multidrug resistance. Univariate and multivariate logistic regression analyses were performed to identify independent risk factors. A nomogram was constructed based on these risk factors, and its performance was evaluated using calibration curves, receiver operating characteristic (ROC) curves, and decision curve analysis (DCA).ResultsA total of 266 patients were included, with 157 (59.02%) testing positive for multidrug-resistant infections. We isolated 329 pathogenic strains: 84 Gram-positive (25.53%), 215 Gram-negative (65.35%), and 30 fungal strains (9.11%). The most common isolate was Klebsiella pneumoniae (57 strains, 17.33%). Patients were divided into a training cohort (n = 177) and a validation cohort (n = 89). Multivariate analysis identified six key indicators: secondary surgery, length of hospital stay, preoperative white blood cell (WBC) count, preoperative neutrophil count, postoperative WBC count, and postoperative C-reactive protein (CRP) levels. The nomogram demonstrated excellent predictive ability, with an area under the curve (AUC) of 0.905 in the training cohort and 0.793 in the validation cohort. Calibration curves indicated high consistency between predicted probabilities and observed values. DCA confirmed the clinical utility of the nomogram.ConclusionOur study shows that multidrug-resistant infections in patients with gastrointestinal fistulas are predominantly caused by Gram-negative bacilli, especially carbapenem-resistant Enterobacteriaceae. Key risk factors include secondary surgery and various blood count parameters. The developed nomogram provides robust predictive accuracy, aiding healthcare providers in implementing targeted infection prevention strategies.
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spelling doaj-art-68e9de5c208041d88003b208c8e3f0b42024-11-28T06:33:15ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882024-11-011410.3389/fcimb.2024.15025291502529Development a risk prediction nomogram for multidrug−resistant bacterial and fungal infection in gastrointestinal fistula patients during the perioperative periodMingming Yin0Haoyi Zheng1Haoyi Zheng2Lifeng Xu3Rong Jin4Xiangyang Wang5Yi Man6Kai Xu7Qiang Ruan8Ting Wang9Kai Guo10Zheng Zhou11Wenyong Wu12Guosheng Gu13Guosheng Gu14Department of General Surgery, Anhui No.2 Provincial People’s Hospital, Hefei, ChinaDepartment of General Surgery, Anhui No.2 Provincial People’s Hospital, Hefei, ChinaDepartment of General Surgery, The Graduate School of Bengbu Medical University, Bengbu, ChinaDepartment of General Surgery, Anhui No.2 Provincial People’s Hospital, Hefei, ChinaDepartment of General Surgery, Anhui No.2 Provincial People’s Hospital, Hefei, ChinaDepartment of General Surgery, Anhui No.2 Provincial People’s Hospital, Hefei, ChinaDepartment of General Surgery, Anhui No.2 Provincial People’s Hospital, Hefei, ChinaDepartment of General Surgery, Anhui No.2 Provincial People’s Hospital, Hefei, ChinaDepartment of General Surgery, Anhui No.2 Provincial People’s Hospital, Hefei, ChinaDepartment of General Surgery, Anhui No.2 Provincial People’s Hospital, Hefei, ChinaDepartment of General Surgery, Anhui No.2 Provincial People’s Hospital, Hefei, ChinaDepartment of General Surgery, Anhui No.2 Provincial People’s Hospital, Hefei, ChinaDepartment of General Surgery, Anhui No.2 Provincial People’s Hospital, Hefei, ChinaDepartment of General Surgery, Anhui No.2 Provincial People’s Hospital, Hefei, ChinaDepartment of General Surgery, The Graduate School of Bengbu Medical University, Bengbu, ChinaBackgroundThis study aims to develop a risk prediction model for multidrug-resistant bacterial and fungal infections in patients with gastrointestinal fistulas during the perioperative period.MethodsA retrospective cohort study was conducted at Anhui No. 2 Provincial People’s Hospital from January 2022 to July 2024. We analyzed the distribution, resistance patterns, and mechanisms of multidrug resistance. Univariate and multivariate logistic regression analyses were performed to identify independent risk factors. A nomogram was constructed based on these risk factors, and its performance was evaluated using calibration curves, receiver operating characteristic (ROC) curves, and decision curve analysis (DCA).ResultsA total of 266 patients were included, with 157 (59.02%) testing positive for multidrug-resistant infections. We isolated 329 pathogenic strains: 84 Gram-positive (25.53%), 215 Gram-negative (65.35%), and 30 fungal strains (9.11%). The most common isolate was Klebsiella pneumoniae (57 strains, 17.33%). Patients were divided into a training cohort (n = 177) and a validation cohort (n = 89). Multivariate analysis identified six key indicators: secondary surgery, length of hospital stay, preoperative white blood cell (WBC) count, preoperative neutrophil count, postoperative WBC count, and postoperative C-reactive protein (CRP) levels. The nomogram demonstrated excellent predictive ability, with an area under the curve (AUC) of 0.905 in the training cohort and 0.793 in the validation cohort. Calibration curves indicated high consistency between predicted probabilities and observed values. DCA confirmed the clinical utility of the nomogram.ConclusionOur study shows that multidrug-resistant infections in patients with gastrointestinal fistulas are predominantly caused by Gram-negative bacilli, especially carbapenem-resistant Enterobacteriaceae. Key risk factors include secondary surgery and various blood count parameters. The developed nomogram provides robust predictive accuracy, aiding healthcare providers in implementing targeted infection prevention strategies.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1502529/fullmultidrug-resistant infectionsgastrointestinal fistulasgram-negative bacillinomogramperioperative period
spellingShingle Mingming Yin
Haoyi Zheng
Haoyi Zheng
Lifeng Xu
Rong Jin
Xiangyang Wang
Yi Man
Kai Xu
Qiang Ruan
Ting Wang
Kai Guo
Zheng Zhou
Wenyong Wu
Guosheng Gu
Guosheng Gu
Development a risk prediction nomogram for multidrug−resistant bacterial and fungal infection in gastrointestinal fistula patients during the perioperative period
Frontiers in Cellular and Infection Microbiology
multidrug-resistant infections
gastrointestinal fistulas
gram-negative bacilli
nomogram
perioperative period
title Development a risk prediction nomogram for multidrug−resistant bacterial and fungal infection in gastrointestinal fistula patients during the perioperative period
title_full Development a risk prediction nomogram for multidrug−resistant bacterial and fungal infection in gastrointestinal fistula patients during the perioperative period
title_fullStr Development a risk prediction nomogram for multidrug−resistant bacterial and fungal infection in gastrointestinal fistula patients during the perioperative period
title_full_unstemmed Development a risk prediction nomogram for multidrug−resistant bacterial and fungal infection in gastrointestinal fistula patients during the perioperative period
title_short Development a risk prediction nomogram for multidrug−resistant bacterial and fungal infection in gastrointestinal fistula patients during the perioperative period
title_sort development a risk prediction nomogram for multidrug resistant bacterial and fungal infection in gastrointestinal fistula patients during the perioperative period
topic multidrug-resistant infections
gastrointestinal fistulas
gram-negative bacilli
nomogram
perioperative period
url https://www.frontiersin.org/articles/10.3389/fcimb.2024.1502529/full
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