Environmental sources of Cryptococcus neoformans species complex in Kampala, Uganda: A preliminary study.

<h4>Objectives</h4>We aimed to determine the environmental sources of C. neoformans and C. gattii species complexes in Uganda.<h4>Methods</h4>One hundred fifty (n = 150) environmental specimens of chicken droppings, marabou stork bird droppings and eucalyptus tree barks in Ug...

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Main Authors: Beatrice Achan, Abel Wembabazi, Tonny Luggya, Innocent Robert Ebwongu, Benson Musinguzi, Herbert Itabangi, Henry Kajumbula, David Meya
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0329947
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author Beatrice Achan
Abel Wembabazi
Tonny Luggya
Innocent Robert Ebwongu
Benson Musinguzi
Herbert Itabangi
Henry Kajumbula
David Meya
author_facet Beatrice Achan
Abel Wembabazi
Tonny Luggya
Innocent Robert Ebwongu
Benson Musinguzi
Herbert Itabangi
Henry Kajumbula
David Meya
author_sort Beatrice Achan
collection DOAJ
description <h4>Objectives</h4>We aimed to determine the environmental sources of C. neoformans and C. gattii species complexes in Uganda.<h4>Methods</h4>One hundred fifty (n = 150) environmental specimens of chicken droppings, marabou stork bird droppings and eucalyptus tree barks in Uganda were examined phenotypically. The specimens were grown on Sabouraud dextrose agar and the colonies examined by India ink microscopy, urea hydrolysis and formation of blue colonies on chromogenic L-Canavanine glycine blue bromothymol blue agar of C. gattii species complex.<h4>Results</h4>The prevalence of C. neoformans species complex was 4% (6/150). Of the positive environmental samples from which C. neoformans species complex were isolated; the predominant source was marabou stork droppings; 8% (2/25) followed by eucalyptus tree barks; 3.96% (4/101). However, there was no Cryptococcus gattii species complex; 0% (0/150).<h4>Conclusion</h4>In Uganda, the environmental sources of C. neoformans species complex are marabou stork droppings and eucalyptus tree barks, however, there seems to be no Cryptococcus gattii species complex.
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spelling doaj-art-68d7e54bdc2f43a081e05c2d4ebf02782025-08-23T05:32:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01208e032994710.1371/journal.pone.0329947Environmental sources of Cryptococcus neoformans species complex in Kampala, Uganda: A preliminary study.Beatrice AchanAbel WembabaziTonny LuggyaInnocent Robert EbwonguBenson MusinguziHerbert ItabangiHenry KajumbulaDavid Meya<h4>Objectives</h4>We aimed to determine the environmental sources of C. neoformans and C. gattii species complexes in Uganda.<h4>Methods</h4>One hundred fifty (n = 150) environmental specimens of chicken droppings, marabou stork bird droppings and eucalyptus tree barks in Uganda were examined phenotypically. The specimens were grown on Sabouraud dextrose agar and the colonies examined by India ink microscopy, urea hydrolysis and formation of blue colonies on chromogenic L-Canavanine glycine blue bromothymol blue agar of C. gattii species complex.<h4>Results</h4>The prevalence of C. neoformans species complex was 4% (6/150). Of the positive environmental samples from which C. neoformans species complex were isolated; the predominant source was marabou stork droppings; 8% (2/25) followed by eucalyptus tree barks; 3.96% (4/101). However, there was no Cryptococcus gattii species complex; 0% (0/150).<h4>Conclusion</h4>In Uganda, the environmental sources of C. neoformans species complex are marabou stork droppings and eucalyptus tree barks, however, there seems to be no Cryptococcus gattii species complex.https://doi.org/10.1371/journal.pone.0329947
spellingShingle Beatrice Achan
Abel Wembabazi
Tonny Luggya
Innocent Robert Ebwongu
Benson Musinguzi
Herbert Itabangi
Henry Kajumbula
David Meya
Environmental sources of Cryptococcus neoformans species complex in Kampala, Uganda: A preliminary study.
PLoS ONE
title Environmental sources of Cryptococcus neoformans species complex in Kampala, Uganda: A preliminary study.
title_full Environmental sources of Cryptococcus neoformans species complex in Kampala, Uganda: A preliminary study.
title_fullStr Environmental sources of Cryptococcus neoformans species complex in Kampala, Uganda: A preliminary study.
title_full_unstemmed Environmental sources of Cryptococcus neoformans species complex in Kampala, Uganda: A preliminary study.
title_short Environmental sources of Cryptococcus neoformans species complex in Kampala, Uganda: A preliminary study.
title_sort environmental sources of cryptococcus neoformans species complex in kampala uganda a preliminary study
url https://doi.org/10.1371/journal.pone.0329947
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