Comparative Analysis of Microbiota in Jiani Yaks with Different Rib Structures
The Jiani yak is a nationally renowned species that is known for its meat which is rich in various minerals, amino acids, and proteins. The rumen microbiota plays a critical role in gastrointestinal health and feed degradation, contributing proteins, lipids, and volatile fatty acids (VFAs) essential...
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2024-11-01
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| author | Yangji Cidan Sijia Lu Hongzhuang Wang Jia Wang Munwar Ali Dalia Fouad Farid S. Ataya Yanbin Zhu Wangdui Basang Kun Li |
| author_facet | Yangji Cidan Sijia Lu Hongzhuang Wang Jia Wang Munwar Ali Dalia Fouad Farid S. Ataya Yanbin Zhu Wangdui Basang Kun Li |
| author_sort | Yangji Cidan |
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| description | The Jiani yak is a nationally renowned species that is known for its meat which is rich in various minerals, amino acids, and proteins. The rumen microbiota plays a critical role in gastrointestinal health and feed degradation, contributing proteins, lipids, and volatile fatty acids (VFAs) essential for milk and meat production. However, there is limited knowledge about the microbiota of free-ranging Jiani yaks, especially those with 15 ribs. Rumen fluid samples were collected from yaks with 14 (PL) ribs and 15 (DL) ribs from a slaughterhouse in Jiani County, China. The total DNA of rumen fluid microorganisms was extracted for microbiota sequencing. Our results revealed 643,713 and 656,346 raw sequences in DL and PL animals, respectively, with 611,934 and 622,814 filtered sequences in these two yak groups. We identified 13,498 Amplicon Sequence Variants (ASVs), with 2623 shared between DL and PL animals. The ratio of Bacteroidota to Firmicutes differed between PL (3.04) and DL (2.35) animals. Additionally, 6 phyla and 21 genera showed significant differences between yaks with 14 and 15 ribs, leading to altered microbiota functions, with 51 and 35 notably different MetaCyc and KEGG pathways, respectively. Hence, the microbiota of yaks with 15 ribs differs from those with 14 ribs. Therefore, these microbiota-related comparative investigations will provide insights into yak husbandry practices and genetic selection strategies for their improved productivity in harsh environments. |
| format | Article |
| id | doaj-art-68aba31e4df74f43a399dcb78fca5b09 |
| institution | Kabale University |
| issn | 2075-1729 |
| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-68aba31e4df74f43a399dcb78fca5b092024-11-26T18:10:30ZengMDPI AGLife2075-17292024-11-011411145810.3390/life14111458Comparative Analysis of Microbiota in Jiani Yaks with Different Rib StructuresYangji Cidan0Sijia Lu1Hongzhuang Wang2Jia Wang3Munwar Ali4Dalia Fouad5Farid S. Ataya6Yanbin Zhu7Wangdui Basang8Kun Li9State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Lhasa 850002, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Lhasa 850002, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Zoology, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Biochemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaState Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Lhasa 850002, ChinaState Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Lhasa 850002, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaThe Jiani yak is a nationally renowned species that is known for its meat which is rich in various minerals, amino acids, and proteins. The rumen microbiota plays a critical role in gastrointestinal health and feed degradation, contributing proteins, lipids, and volatile fatty acids (VFAs) essential for milk and meat production. However, there is limited knowledge about the microbiota of free-ranging Jiani yaks, especially those with 15 ribs. Rumen fluid samples were collected from yaks with 14 (PL) ribs and 15 (DL) ribs from a slaughterhouse in Jiani County, China. The total DNA of rumen fluid microorganisms was extracted for microbiota sequencing. Our results revealed 643,713 and 656,346 raw sequences in DL and PL animals, respectively, with 611,934 and 622,814 filtered sequences in these two yak groups. We identified 13,498 Amplicon Sequence Variants (ASVs), with 2623 shared between DL and PL animals. The ratio of Bacteroidota to Firmicutes differed between PL (3.04) and DL (2.35) animals. Additionally, 6 phyla and 21 genera showed significant differences between yaks with 14 and 15 ribs, leading to altered microbiota functions, with 51 and 35 notably different MetaCyc and KEGG pathways, respectively. Hence, the microbiota of yaks with 15 ribs differs from those with 14 ribs. Therefore, these microbiota-related comparative investigations will provide insights into yak husbandry practices and genetic selection strategies for their improved productivity in harsh environments.https://www.mdpi.com/2075-1729/14/11/1458yakJianirib structurerumenmicrobiotasequencing |
| spellingShingle | Yangji Cidan Sijia Lu Hongzhuang Wang Jia Wang Munwar Ali Dalia Fouad Farid S. Ataya Yanbin Zhu Wangdui Basang Kun Li Comparative Analysis of Microbiota in Jiani Yaks with Different Rib Structures Life yak Jiani rib structure rumen microbiota sequencing |
| title | Comparative Analysis of Microbiota in Jiani Yaks with Different Rib Structures |
| title_full | Comparative Analysis of Microbiota in Jiani Yaks with Different Rib Structures |
| title_fullStr | Comparative Analysis of Microbiota in Jiani Yaks with Different Rib Structures |
| title_full_unstemmed | Comparative Analysis of Microbiota in Jiani Yaks with Different Rib Structures |
| title_short | Comparative Analysis of Microbiota in Jiani Yaks with Different Rib Structures |
| title_sort | comparative analysis of microbiota in jiani yaks with different rib structures |
| topic | yak Jiani rib structure rumen microbiota sequencing |
| url | https://www.mdpi.com/2075-1729/14/11/1458 |
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