Proteomic study of the inhibitory effects of tannic acid on MRSA biofilm
IntroductionThe mechanism of tannic acid (TA) intervention on methicillin-resistant Staphylococcus aureus (MRSA, USA 300) biofilm formation was explored using proteomics.MethodsThe minimum inhibitory concentration (MIC) of TA against the MRSA standard strain USA 300 was determined by two-fold serial...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1413669/full |
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| author | Yang Miao Wang Shuang Qu Qianwei Liu Xin Peng Wei Yang Hai Zhou Yonghui Yu Xinbo |
| author_facet | Yang Miao Wang Shuang Qu Qianwei Liu Xin Peng Wei Yang Hai Zhou Yonghui Yu Xinbo |
| author_sort | Yang Miao |
| collection | DOAJ |
| description | IntroductionThe mechanism of tannic acid (TA) intervention on methicillin-resistant Staphylococcus aureus (MRSA, USA 300) biofilm formation was explored using proteomics.MethodsThe minimum inhibitory concentration (MIC) of TA against the MRSA standard strain USA 300 was determined by two-fold serial dilution of the microbroth. The effects of TA were studied using crystal violet staining. The morphology of TA-treated USA 300 cells was observed by scanning electron microscopy and confocal laser scanning microscopy. Differentially expressed proteins (DEPs) were screened using proteomic and biological information analyses, and their transcriptional levels were verified using real-time quantitative polymerase chain reaction.ResultsThe MIC of TA was 0.625 mg/mL, whereas 1/2 MIC (0.3125 mg/mL) of TA significantly inhibited biofilm formation without affecting the bacterial growth (p < 0.01) and prevented the formation of a complete three-dimensional biofilm structure. Using 1/2 MIC of TA, 208 DEPs were identified, of which 127 were upregulated and 81 were downregulated. The transcriptional levels of the genes corresponding to five randomly selected DEPs (glnA, ribD, clpB, gap, and lukE) were consistent with the proteomics data (p < 0.05). Bioinformatic analysis showed that the changes in the MRSA strains after TA intervention primarily involved pyrimidine and purine metabolisms, arginine biosynthesis, and the citric acid cycle.ConclusionTA exerts an antibacterial effect on MRSA and can be used as a potential candidate for the development of anti-biofilm drugs, thereby laying a foundation for the treatment of MRSA biofilm-induced infections. |
| format | Article |
| id | doaj-art-68aaed87a54a45e88bb2cb22f4c64e09 |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj-art-68aaed87a54a45e88bb2cb22f4c64e092024-12-18T04:25:12ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122024-12-011510.3389/fphar.2024.14136691413669Proteomic study of the inhibitory effects of tannic acid on MRSA biofilmYang Miao0Wang Shuang1Qu Qianwei2Liu Xin3Peng Wei4Yang Hai5Zhou Yonghui6Yu Xinbo7School of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, ChinaSchool of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, ChinaSchool of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, ChinaSchool of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, ChinaDepartment of Pathology, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, ChinaSchool of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, ChinaSchool of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, ChinaIntroductionThe mechanism of tannic acid (TA) intervention on methicillin-resistant Staphylococcus aureus (MRSA, USA 300) biofilm formation was explored using proteomics.MethodsThe minimum inhibitory concentration (MIC) of TA against the MRSA standard strain USA 300 was determined by two-fold serial dilution of the microbroth. The effects of TA were studied using crystal violet staining. The morphology of TA-treated USA 300 cells was observed by scanning electron microscopy and confocal laser scanning microscopy. Differentially expressed proteins (DEPs) were screened using proteomic and biological information analyses, and their transcriptional levels were verified using real-time quantitative polymerase chain reaction.ResultsThe MIC of TA was 0.625 mg/mL, whereas 1/2 MIC (0.3125 mg/mL) of TA significantly inhibited biofilm formation without affecting the bacterial growth (p < 0.01) and prevented the formation of a complete three-dimensional biofilm structure. Using 1/2 MIC of TA, 208 DEPs were identified, of which 127 were upregulated and 81 were downregulated. The transcriptional levels of the genes corresponding to five randomly selected DEPs (glnA, ribD, clpB, gap, and lukE) were consistent with the proteomics data (p < 0.05). Bioinformatic analysis showed that the changes in the MRSA strains after TA intervention primarily involved pyrimidine and purine metabolisms, arginine biosynthesis, and the citric acid cycle.ConclusionTA exerts an antibacterial effect on MRSA and can be used as a potential candidate for the development of anti-biofilm drugs, thereby laying a foundation for the treatment of MRSA biofilm-induced infections.https://www.frontiersin.org/articles/10.3389/fphar.2024.1413669/fulltannic acidmethicillin-resistant Staphylococcus aureusbiofilmproteomicsinhibition |
| spellingShingle | Yang Miao Wang Shuang Qu Qianwei Liu Xin Peng Wei Yang Hai Zhou Yonghui Yu Xinbo Proteomic study of the inhibitory effects of tannic acid on MRSA biofilm Frontiers in Pharmacology tannic acid methicillin-resistant Staphylococcus aureus biofilm proteomics inhibition |
| title | Proteomic study of the inhibitory effects of tannic acid on MRSA biofilm |
| title_full | Proteomic study of the inhibitory effects of tannic acid on MRSA biofilm |
| title_fullStr | Proteomic study of the inhibitory effects of tannic acid on MRSA biofilm |
| title_full_unstemmed | Proteomic study of the inhibitory effects of tannic acid on MRSA biofilm |
| title_short | Proteomic study of the inhibitory effects of tannic acid on MRSA biofilm |
| title_sort | proteomic study of the inhibitory effects of tannic acid on mrsa biofilm |
| topic | tannic acid methicillin-resistant Staphylococcus aureus biofilm proteomics inhibition |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1413669/full |
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