Exploring salinity induced adaptations in marine diatoms using advanced photonic techniques
Abstract Photonic-based methods are crucial in biology and medicine due to their non-invasive nature, allowing remote measurements without affecting biological specimens. The study of diatoms using advanced photonic methods remains a relatively underexplored area, presenting significant opportunitie...
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Nature Portfolio
2024-12-01
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| Online Access: | https://doi.org/10.1038/s41598-024-83640-9 |
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| author | Julijana Cvjetinovic Yekaterina D. Bedoshvili Nickolai A. Davidovich Eugene G. Maksimov Ekaterina S. Prikhozhdenko Daria A. Todorenko Daria V. Bodunova Olga I. Davidovich Igor S. Sergeev Dmitry A. Gorin |
| author_facet | Julijana Cvjetinovic Yekaterina D. Bedoshvili Nickolai A. Davidovich Eugene G. Maksimov Ekaterina S. Prikhozhdenko Daria A. Todorenko Daria V. Bodunova Olga I. Davidovich Igor S. Sergeev Dmitry A. Gorin |
| author_sort | Julijana Cvjetinovic |
| collection | DOAJ |
| description | Abstract Photonic-based methods are crucial in biology and medicine due to their non-invasive nature, allowing remote measurements without affecting biological specimens. The study of diatoms using advanced photonic methods remains a relatively underexplored area, presenting significant opportunities for pioneering discoveries. This research provides a comprehensive analysis of marine diatoms, specifically Nitzschia sp., across varying salinity levels, integrating fluorescence lifetime imaging microscopy (FLIM), combined photoacoustic and fluorescence tomographies (PAFT), and ultrastructural examinations using transmission electron microscopy. Key findings include a systematic shift in the mean fluorescence lifetime from 570 ps at 20‰ to 940 ps at 80‰, indicating functional adaptations in chlorophyll molecules within light-harvesting complexes. At 60‰ salinity, anomalies are observed in the development of silica valves and polysaccharide layers, suggesting abnormalities in valve morphogenesis. Lipid droplets within the cells display a minimum diameter at 40‰, indicating metabolic adjustments to osmotic stress. The intensity of both fluorescence and photoacoustic signals increases with increasing salinity levels. These insights enhance understanding of the ecological implications of salinity stress on diatom communities and pave the way for future research on leveraging the unique adaptive mechanisms of microalgae for environmental monitoring and sustainable biotechnological applications. |
| format | Article |
| id | doaj-art-685dd24d259b4fb18611190e50f2fb81 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-685dd24d259b4fb18611190e50f2fb812025-01-05T12:28:17ZengNature PortfolioScientific Reports2045-23222024-12-0114111710.1038/s41598-024-83640-9Exploring salinity induced adaptations in marine diatoms using advanced photonic techniquesJulijana Cvjetinovic0Yekaterina D. Bedoshvili1Nickolai A. Davidovich2Eugene G. Maksimov3Ekaterina S. Prikhozhdenko4Daria A. Todorenko5Daria V. Bodunova6Olga I. Davidovich7Igor S. Sergeev8Dmitry A. Gorin9Center for Photonic Science and Engineering, Skolkovo Institute of Science and TechnologyCenter for Photonic Science and Engineering, Skolkovo Institute of Science and TechnologyCenter for Photonic Science and Engineering, Skolkovo Institute of Science and TechnologyDepartment of Biology, M.V. Lomonosov Moscow State UniversitySaratov State UniversityDepartment of Biology, M.V. Lomonosov Moscow State UniversityDepartment of Biology, M.V. Lomonosov Moscow State UniversityT. I. Vyazemsky Karadag Scientific Station, Natural Reserve of the Russian Academy of SciencesCenter for Photonic Science and Engineering, Skolkovo Institute of Science and TechnologyCenter for Photonic Science and Engineering, Skolkovo Institute of Science and TechnologyAbstract Photonic-based methods are crucial in biology and medicine due to their non-invasive nature, allowing remote measurements without affecting biological specimens. The study of diatoms using advanced photonic methods remains a relatively underexplored area, presenting significant opportunities for pioneering discoveries. This research provides a comprehensive analysis of marine diatoms, specifically Nitzschia sp., across varying salinity levels, integrating fluorescence lifetime imaging microscopy (FLIM), combined photoacoustic and fluorescence tomographies (PAFT), and ultrastructural examinations using transmission electron microscopy. Key findings include a systematic shift in the mean fluorescence lifetime from 570 ps at 20‰ to 940 ps at 80‰, indicating functional adaptations in chlorophyll molecules within light-harvesting complexes. At 60‰ salinity, anomalies are observed in the development of silica valves and polysaccharide layers, suggesting abnormalities in valve morphogenesis. Lipid droplets within the cells display a minimum diameter at 40‰, indicating metabolic adjustments to osmotic stress. The intensity of both fluorescence and photoacoustic signals increases with increasing salinity levels. These insights enhance understanding of the ecological implications of salinity stress on diatom communities and pave the way for future research on leveraging the unique adaptive mechanisms of microalgae for environmental monitoring and sustainable biotechnological applications.https://doi.org/10.1038/s41598-024-83640-9Fluorescence lifetime imagingPhotoacoustic imagingUltrastructureSalinityDiatoms |
| spellingShingle | Julijana Cvjetinovic Yekaterina D. Bedoshvili Nickolai A. Davidovich Eugene G. Maksimov Ekaterina S. Prikhozhdenko Daria A. Todorenko Daria V. Bodunova Olga I. Davidovich Igor S. Sergeev Dmitry A. Gorin Exploring salinity induced adaptations in marine diatoms using advanced photonic techniques Scientific Reports Fluorescence lifetime imaging Photoacoustic imaging Ultrastructure Salinity Diatoms |
| title | Exploring salinity induced adaptations in marine diatoms using advanced photonic techniques |
| title_full | Exploring salinity induced adaptations in marine diatoms using advanced photonic techniques |
| title_fullStr | Exploring salinity induced adaptations in marine diatoms using advanced photonic techniques |
| title_full_unstemmed | Exploring salinity induced adaptations in marine diatoms using advanced photonic techniques |
| title_short | Exploring salinity induced adaptations in marine diatoms using advanced photonic techniques |
| title_sort | exploring salinity induced adaptations in marine diatoms using advanced photonic techniques |
| topic | Fluorescence lifetime imaging Photoacoustic imaging Ultrastructure Salinity Diatoms |
| url | https://doi.org/10.1038/s41598-024-83640-9 |
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