Lacticaseibacillus paracasei K56 inhibits lipid accumulation in adipocytes by promoting lipolysis
Probiotics crosstalk immunity to improve host glycolipid metabolism, which is a new strategy for obesity. This study aimed to explore the functions of Lacticaseibacillus paracasei K56 (K56), in regulating the lipid metabolism of adipocytes and its immune regulation mechanisms. We co-cultured RAW264....
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Tsinghua University Press
2024-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2023.9250034 |
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| author | Silu Wang Jinxing Li Wei-Hsien Liu Niya Li Huijing Liang Weilian Hung Qiuyue Jiang Ruyue Cheng Xi Shen Fang He |
| author_facet | Silu Wang Jinxing Li Wei-Hsien Liu Niya Li Huijing Liang Weilian Hung Qiuyue Jiang Ruyue Cheng Xi Shen Fang He |
| author_sort | Silu Wang |
| collection | DOAJ |
| description | Probiotics crosstalk immunity to improve host glycolipid metabolism, which is a new strategy for obesity. This study aimed to explore the functions of Lacticaseibacillus paracasei K56 (K56), in regulating the lipid metabolism of adipocytes and its immune regulation mechanisms. We co-cultured RAW264.7 macrophages with K56, and the K56-stimulated RAW264.7-conditioned medium (K56-CM) was collected and treated with 3T3-L1 pre-adipocytes. The expression of lipid metabolism-related markers of adipocytes, and the content of cytokines in CMs were detected. The results demonstrated that K56-CM promoted the expression of peroxisome proliferator-activated receptor-γ (PPAR-γ), CCAAT/enhancer binding protein α (C/EBPα), and other adipogenesis-related markers, which resulted in the upregulation of lipolysis markers, such as hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL). The activation of lipolysis enhanced the expression of fatty acid β-oxidation-related and mitochondrial biogenesis-related markers and reduced lipid accumulation in adipocytes. The glycolipid metabolism pattern of K56 may be due to its immunomodulatory characteristics, which stimulated macrophages to secrete fewer TNF-α, thereby promoting the expression of lipolysis-related markers, and TNF-α synergized with lipase to promote lipolysis. It has not been reported that L. paracasei modulated lipid metabolism via the lipolysis pathway, which suggested that K56 may regulate glycolipid metabolism of the host by maintaining immune homeostasis. |
| format | Article |
| id | doaj-art-6857cf1abe8949e685a471701fb0200d |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-6857cf1abe8949e685a471701fb0200d2025-01-10T06:57:02ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-11-011363511352110.26599/FSHW.2023.9250034Lacticaseibacillus paracasei K56 inhibits lipid accumulation in adipocytes by promoting lipolysisSilu Wang0Jinxing Li1Wei-Hsien Liu2Niya Li3Huijing Liang4Weilian Hung5Qiuyue Jiang6Ruyue Cheng7Xi Shen8Fang He9Department of Nutrition and Food Hygiene, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, ChinaDepartment of Nutrition and Food Hygiene, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, ChinaInner Mongolia Yili Industrial Group Co., Ltd., Hohhot 010110, ChinaDepartment of Nutrition and Food Hygiene, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, ChinaDepartment of Nutrition and Food Hygiene, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, ChinaInner Mongolia Yili Industrial Group Co., Ltd., Hohhot 010110, ChinaInner Mongolia Yili Industrial Group Co., Ltd., Hohhot 010110, ChinaDepartment of Nutrition and Food Hygiene, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, ChinaDepartment of Nutrition and Food Hygiene, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, ChinaDepartment of Nutrition and Food Hygiene, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, ChinaProbiotics crosstalk immunity to improve host glycolipid metabolism, which is a new strategy for obesity. This study aimed to explore the functions of Lacticaseibacillus paracasei K56 (K56), in regulating the lipid metabolism of adipocytes and its immune regulation mechanisms. We co-cultured RAW264.7 macrophages with K56, and the K56-stimulated RAW264.7-conditioned medium (K56-CM) was collected and treated with 3T3-L1 pre-adipocytes. The expression of lipid metabolism-related markers of adipocytes, and the content of cytokines in CMs were detected. The results demonstrated that K56-CM promoted the expression of peroxisome proliferator-activated receptor-γ (PPAR-γ), CCAAT/enhancer binding protein α (C/EBPα), and other adipogenesis-related markers, which resulted in the upregulation of lipolysis markers, such as hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL). The activation of lipolysis enhanced the expression of fatty acid β-oxidation-related and mitochondrial biogenesis-related markers and reduced lipid accumulation in adipocytes. The glycolipid metabolism pattern of K56 may be due to its immunomodulatory characteristics, which stimulated macrophages to secrete fewer TNF-α, thereby promoting the expression of lipolysis-related markers, and TNF-α synergized with lipase to promote lipolysis. It has not been reported that L. paracasei modulated lipid metabolism via the lipolysis pathway, which suggested that K56 may regulate glycolipid metabolism of the host by maintaining immune homeostasis.https://www.sciopen.com/article/10.26599/FSHW.2023.9250034obesitylacticaseibacillusmacrophageadipocytelipolysis |
| spellingShingle | Silu Wang Jinxing Li Wei-Hsien Liu Niya Li Huijing Liang Weilian Hung Qiuyue Jiang Ruyue Cheng Xi Shen Fang He Lacticaseibacillus paracasei K56 inhibits lipid accumulation in adipocytes by promoting lipolysis Food Science and Human Wellness obesity lacticaseibacillus macrophage adipocyte lipolysis |
| title | Lacticaseibacillus paracasei K56 inhibits lipid accumulation in adipocytes by promoting lipolysis |
| title_full | Lacticaseibacillus paracasei K56 inhibits lipid accumulation in adipocytes by promoting lipolysis |
| title_fullStr | Lacticaseibacillus paracasei K56 inhibits lipid accumulation in adipocytes by promoting lipolysis |
| title_full_unstemmed | Lacticaseibacillus paracasei K56 inhibits lipid accumulation in adipocytes by promoting lipolysis |
| title_short | Lacticaseibacillus paracasei K56 inhibits lipid accumulation in adipocytes by promoting lipolysis |
| title_sort | lacticaseibacillus paracasei k56 inhibits lipid accumulation in adipocytes by promoting lipolysis |
| topic | obesity lacticaseibacillus macrophage adipocyte lipolysis |
| url | https://www.sciopen.com/article/10.26599/FSHW.2023.9250034 |
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