Production of Alpha Amylase from Banana Peel Using Aspergillus niger
Background: Various chemicals and dyes used in paper production are xenobiotic in nature and pose significant environmental concerns. Objective: In this study, a fungal strain was isolated from contaminated paper mill effluent and identified as Aspergillus niger through standard microbiological...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Jinnah University for Women
2025-08-01
|
| Series: | RADS Journal of Biological Research & Applied Science |
| Subjects: | |
| Online Access: | https://jbas.juw.edu.pk/index.php/JBAS/article/view/739 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849239822239006720 |
|---|---|
| author | Ibrahim Khan Mir Sadiq Shah |
| author_facet | Ibrahim Khan Mir Sadiq Shah |
| author_sort | Ibrahim Khan |
| collection | DOAJ |
| description |
Background: Various chemicals and dyes used in paper production are xenobiotic in nature and pose significant environmental concerns.
Objective: In this study, a fungal strain was isolated from contaminated paper mill effluent and identified as Aspergillus niger through standard microbiological and morphological analyses.
Methodology: The strain was screened for ?-amylase production using banana peel—a low-cost agro-residue—as the sole carbon source. Optimization of physicochemical parameters, including incubation temperature (30 °C), initial pH (6.0), and incubation time (72 h), yielded maximal enzyme activity. Crude ?-amylase activity was quantified spectrophotometrically by measuring reducing sugars released from soluble starch. Partial purification was achieved via ammonium sulfate precipitation, with 50% saturation providing the highest specific activity and yield.
Application: The applicability of the partially purified ?-amylase was demonstrated by de-inking waste office paper; treatment with the enzyme significantly improved paper brightness and reduced ink load, as confirmed by standard gravimetric and spectrophotometric assays.
Conclusion: These findings indicate that banana peel is a feasible and cost-efficient substrate for ?-amylase production by A. niger. The ?-amylase has promising potential for sustainable paper waste management and bioremediation in the pulp and paper industry.
|
| format | Article |
| id | doaj-art-682c49a37b7f4fcc8e5ff6e961b945cf |
| institution | Kabale University |
| issn | 2305-8722 2521-8573 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Jinnah University for Women |
| record_format | Article |
| series | RADS Journal of Biological Research & Applied Science |
| spelling | doaj-art-682c49a37b7f4fcc8e5ff6e961b945cf2025-08-20T04:00:50ZengJinnah University for WomenRADS Journal of Biological Research & Applied Science2305-87222521-85732025-08-01161Production of Alpha Amylase from Banana Peel Using Aspergillus nigerIbrahim Khan0Mir Sadiq Shah1Department of Biotechnology, Bannu University of Science and Technology, Bannu, Pakistan.Department of Zoology, Bannu University of Science and Technology, Bannu, Pakistan. Background: Various chemicals and dyes used in paper production are xenobiotic in nature and pose significant environmental concerns. Objective: In this study, a fungal strain was isolated from contaminated paper mill effluent and identified as Aspergillus niger through standard microbiological and morphological analyses. Methodology: The strain was screened for ?-amylase production using banana peel—a low-cost agro-residue—as the sole carbon source. Optimization of physicochemical parameters, including incubation temperature (30 °C), initial pH (6.0), and incubation time (72 h), yielded maximal enzyme activity. Crude ?-amylase activity was quantified spectrophotometrically by measuring reducing sugars released from soluble starch. Partial purification was achieved via ammonium sulfate precipitation, with 50% saturation providing the highest specific activity and yield. Application: The applicability of the partially purified ?-amylase was demonstrated by de-inking waste office paper; treatment with the enzyme significantly improved paper brightness and reduced ink load, as confirmed by standard gravimetric and spectrophotometric assays. Conclusion: These findings indicate that banana peel is a feasible and cost-efficient substrate for ?-amylase production by A. niger. The ?-amylase has promising potential for sustainable paper waste management and bioremediation in the pulp and paper industry. https://jbas.juw.edu.pk/index.php/JBAS/article/view/739Alpha AmylaseAspergillus nigerPurification |
| spellingShingle | Ibrahim Khan Mir Sadiq Shah Production of Alpha Amylase from Banana Peel Using Aspergillus niger RADS Journal of Biological Research & Applied Science Alpha Amylase Aspergillus niger Purification |
| title | Production of Alpha Amylase from Banana Peel Using Aspergillus niger |
| title_full | Production of Alpha Amylase from Banana Peel Using Aspergillus niger |
| title_fullStr | Production of Alpha Amylase from Banana Peel Using Aspergillus niger |
| title_full_unstemmed | Production of Alpha Amylase from Banana Peel Using Aspergillus niger |
| title_short | Production of Alpha Amylase from Banana Peel Using Aspergillus niger |
| title_sort | production of alpha amylase from banana peel using aspergillus niger |
| topic | Alpha Amylase Aspergillus niger Purification |
| url | https://jbas.juw.edu.pk/index.php/JBAS/article/view/739 |
| work_keys_str_mv | AT ibrahimkhan productionofalphaamylasefrombananapeelusingaspergillusniger AT mirsadiqshah productionofalphaamylasefrombananapeelusingaspergillusniger |