Bacteriophages RCF and 1-6bf can control the growth of avian pathogenic Escherichia coli
Escherichia coli (E. coli) is a widely distributed pathogenic bacterium that poses a substantial hazard to poultry, leading to the development of a severe systemic disease known as colibacillosis. Colibacillosis is involved in multimillion-dollar losses to the poultry industry each year worldwide. A...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125000276 |
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| author | Farah Khan Hafsa Naseem Muhammad Asif Iqbal Alvi Shafiq ur Rehman Abdul Rehman |
| author_facet | Farah Khan Hafsa Naseem Muhammad Asif Iqbal Alvi Shafiq ur Rehman Abdul Rehman |
| author_sort | Farah Khan |
| collection | DOAJ |
| description | Escherichia coli (E. coli) is a widely distributed pathogenic bacterium that poses a substantial hazard to poultry, leading to the development of a severe systemic disease known as colibacillosis. Colibacillosis is involved in multimillion-dollar losses to the poultry industry each year worldwide. Avian pathogenic E. coli is also involved in causing meningitis and urinary tract infections in humans. This creates a significant risk to public health. The increasing incidence of multidrug-resistant illnesses and the failure of antibiotics in human and veterinary medicine have led to a pressing demand for alternate approaches. This study investigates the possibility of bacteriophages as an acceptable substitute for antibiotics in managing E. coli infections in poultry. In the current study, two novel phages targeting E. coli (EP1) strain were isolated from sewage water and thoroughly characterized in vitro. Transmission electron microscopy reveals that Rcf and 1-6bf belong to the ''Podoviridae'' and ''Caudovirales''. Rcf has an icosahedral capsid of 18 nm with a tail size of 5 nm, while 1-6bf has an elongated head capsid of 93 nm and a short non-contractile tail of 8 nm with tail fibers for attachment. RCF and 1-6bf have genome sizes of 38 kb and 77 kb, with GC content of 50.98 % and 42.1 % respectively. Notably, phage 1-6bf displayed remarkable tolerance to high temperatures, retaining lytic activity at 95°C. Both phages effectively controlled host bacterial growth for up to 12 h post-infection. Rcf and 1-6bf produce clear plaques with a latent period of 10 min and 5 min with a burst size of 85 and 220 PFU/cell respectively. |
| format | Article |
| id | doaj-art-67d16d4928054fe6939d7998e20d1e42 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-67d16d4928054fe6939d7998e20d1e422025-01-14T04:12:00ZengElsevierPoultry Science0032-57912025-02-011042104790Bacteriophages RCF and 1-6bf can control the growth of avian pathogenic Escherichia coliFarah Khan0Hafsa Naseem1Muhammad Asif2Iqbal Alvi3Shafiq ur Rehman4Abdul Rehman5Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, PakistanInstitute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, PakistanInstitute of Industrial Biotechnology, Govt. College University Lahore, PakistanDepartment of Microbiology, Hazara University, Mansehra, PakistanInstitute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, PakistanInstitute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan; Correspondence author at: Institute of Microbiology & Molecular Genetics, University of the Punjab, New Campus, Lahore 54590, Pakistan.Escherichia coli (E. coli) is a widely distributed pathogenic bacterium that poses a substantial hazard to poultry, leading to the development of a severe systemic disease known as colibacillosis. Colibacillosis is involved in multimillion-dollar losses to the poultry industry each year worldwide. Avian pathogenic E. coli is also involved in causing meningitis and urinary tract infections in humans. This creates a significant risk to public health. The increasing incidence of multidrug-resistant illnesses and the failure of antibiotics in human and veterinary medicine have led to a pressing demand for alternate approaches. This study investigates the possibility of bacteriophages as an acceptable substitute for antibiotics in managing E. coli infections in poultry. In the current study, two novel phages targeting E. coli (EP1) strain were isolated from sewage water and thoroughly characterized in vitro. Transmission electron microscopy reveals that Rcf and 1-6bf belong to the ''Podoviridae'' and ''Caudovirales''. Rcf has an icosahedral capsid of 18 nm with a tail size of 5 nm, while 1-6bf has an elongated head capsid of 93 nm and a short non-contractile tail of 8 nm with tail fibers for attachment. RCF and 1-6bf have genome sizes of 38 kb and 77 kb, with GC content of 50.98 % and 42.1 % respectively. Notably, phage 1-6bf displayed remarkable tolerance to high temperatures, retaining lytic activity at 95°C. Both phages effectively controlled host bacterial growth for up to 12 h post-infection. Rcf and 1-6bf produce clear plaques with a latent period of 10 min and 5 min with a burst size of 85 and 220 PFU/cell respectively.http://www.sciencedirect.com/science/article/pii/S0032579125000276CharacterizationBacteriophagein vitroBacterial growth reduction |
| spellingShingle | Farah Khan Hafsa Naseem Muhammad Asif Iqbal Alvi Shafiq ur Rehman Abdul Rehman Bacteriophages RCF and 1-6bf can control the growth of avian pathogenic Escherichia coli Poultry Science Characterization Bacteriophage in vitro Bacterial growth reduction |
| title | Bacteriophages RCF and 1-6bf can control the growth of avian pathogenic Escherichia coli |
| title_full | Bacteriophages RCF and 1-6bf can control the growth of avian pathogenic Escherichia coli |
| title_fullStr | Bacteriophages RCF and 1-6bf can control the growth of avian pathogenic Escherichia coli |
| title_full_unstemmed | Bacteriophages RCF and 1-6bf can control the growth of avian pathogenic Escherichia coli |
| title_short | Bacteriophages RCF and 1-6bf can control the growth of avian pathogenic Escherichia coli |
| title_sort | bacteriophages rcf and 1 6bf can control the growth of avian pathogenic escherichia coli |
| topic | Characterization Bacteriophage in vitro Bacterial growth reduction |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125000276 |
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