Impact of consumption of the human milk oligosaccharides 2′-FL and LNnT on reduction of risk of allergic sensitisation

Human Milk Oligosaccharides (HMOs) have been proposed to be instrumental in building immune competence. To explore the role of HMOs in allergy prevention, twenty-one HMOs were quantified in breast milk samples and associated with sensitisation in infants. 2′-fucosyllactose (2′-FL) levels were positi...

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Main Authors: Sébastien Holvoet, Francis Foata, Sophie Nutten, Ellen Ní Cléirigh, Maya Shevlyakova, Cheong Kwong Chung, Gregory Lefevre, Norbert Sprenger, Tristan Bourdeau, Dominique Donnicola, Mario Noti, Severine Combremont, Aristea Binia, Chiara Nembrini, Mandy Vogel, Wieland Kiess, Carine Blanchard
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Food and Agricultural Immunology
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Online Access:https://www.tandfonline.com/doi/10.1080/09540105.2023.2301703
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author Sébastien Holvoet
Francis Foata
Sophie Nutten
Ellen Ní Cléirigh
Maya Shevlyakova
Cheong Kwong Chung
Gregory Lefevre
Norbert Sprenger
Tristan Bourdeau
Dominique Donnicola
Mario Noti
Severine Combremont
Aristea Binia
Chiara Nembrini
Mandy Vogel
Wieland Kiess
Carine Blanchard
author_facet Sébastien Holvoet
Francis Foata
Sophie Nutten
Ellen Ní Cléirigh
Maya Shevlyakova
Cheong Kwong Chung
Gregory Lefevre
Norbert Sprenger
Tristan Bourdeau
Dominique Donnicola
Mario Noti
Severine Combremont
Aristea Binia
Chiara Nembrini
Mandy Vogel
Wieland Kiess
Carine Blanchard
author_sort Sébastien Holvoet
collection DOAJ
description Human Milk Oligosaccharides (HMOs) have been proposed to be instrumental in building immune competence. To explore the role of HMOs in allergy prevention, twenty-one HMOs were quantified in breast milk samples and associated with sensitisation in infants. 2′-fucosyllactose (2′-FL) levels were positively associated with an increased risk of sensitisation, atopic dermatitis and recurrent skin rash. Interestingly, 2′-FL levels, ranging from 1.35 to 1.95 g/L, were associated with a higher prevalence of non-allergic and non-sensitised infants. The role of 2′-FL and lacto-N-neotetraose (LNnT) was further investigated in allergic sensitisation models in vivo. Oral administration of HMOs decreased allergic sensitisation. This was associated with gut microbiota and short-chain fatty acid (SCFA) production changes. Aligned with the clinical associations, the decreased sensitisation was not observed with lower and higher tested doses of the HMOs supporting a U-shape association between 2′-FL and LNnT levels and allergic sensitisation risk reduction in humans and mice.Trial registration: ClinicalTrials.gov identifier: NCT02550236.
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spelling doaj-art-676d9ae488df4a1096975c4c838c91f42024-12-20T03:35:56ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432024-12-0135110.1080/09540105.2023.2301703Impact of consumption of the human milk oligosaccharides 2′-FL and LNnT on reduction of risk of allergic sensitisationSébastien Holvoet0Francis Foata1Sophie Nutten2Ellen Ní Cléirigh3Maya Shevlyakova4Cheong Kwong Chung5Gregory Lefevre6Norbert Sprenger7Tristan Bourdeau8Dominique Donnicola9Mario Noti10Severine Combremont11Aristea Binia12Chiara Nembrini13Mandy Vogel14Wieland Kiess15Carine Blanchard16Nestlé Institute of Health Sciences, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, SwitzerlandNestlé Institute of Health Sciences, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, SwitzerlandNestlé Health Science, Lausanne, SwitzerlandNestlé Development Centre Nutrition, Askeaton, IrelandNestlé Institute of Health Sciences, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, SwitzerlandNestlé Institute of Health Sciences, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, SwitzerlandNestlé Institute of Health Sciences, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, SwitzerlandNestlé Institute of Health Sciences, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, SwitzerlandNestlé Institute of Health Sciences, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, SwitzerlandNestlé Institute of Health Sciences, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, SwitzerlandNestlé Institute of Health Sciences, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, SwitzerlandNestlé Institute of Health Sciences, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, SwitzerlandNestlé Institute of Health Sciences, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, SwitzerlandNestlé Institute of Health Sciences, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, SwitzerlandFaculty of Medicine, LIFE Leipzig Research Center for Civilization Diseases, University of Leipzig, Leipzig, GermanyFaculty of Medicine, LIFE Leipzig Research Center for Civilization Diseases, University of Leipzig, Leipzig, GermanyNestlé Institute of Health Sciences, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, SwitzerlandHuman Milk Oligosaccharides (HMOs) have been proposed to be instrumental in building immune competence. To explore the role of HMOs in allergy prevention, twenty-one HMOs were quantified in breast milk samples and associated with sensitisation in infants. 2′-fucosyllactose (2′-FL) levels were positively associated with an increased risk of sensitisation, atopic dermatitis and recurrent skin rash. Interestingly, 2′-FL levels, ranging from 1.35 to 1.95 g/L, were associated with a higher prevalence of non-allergic and non-sensitised infants. The role of 2′-FL and lacto-N-neotetraose (LNnT) was further investigated in allergic sensitisation models in vivo. Oral administration of HMOs decreased allergic sensitisation. This was associated with gut microbiota and short-chain fatty acid (SCFA) production changes. Aligned with the clinical associations, the decreased sensitisation was not observed with lower and higher tested doses of the HMOs supporting a U-shape association between 2′-FL and LNnT levels and allergic sensitisation risk reduction in humans and mice.Trial registration: ClinicalTrials.gov identifier: NCT02550236.https://www.tandfonline.com/doi/10.1080/09540105.2023.2301703breast milkhuman milk oligosaccharidessensitisationatopic dermatitisfood allergyinfant nutrition
spellingShingle Sébastien Holvoet
Francis Foata
Sophie Nutten
Ellen Ní Cléirigh
Maya Shevlyakova
Cheong Kwong Chung
Gregory Lefevre
Norbert Sprenger
Tristan Bourdeau
Dominique Donnicola
Mario Noti
Severine Combremont
Aristea Binia
Chiara Nembrini
Mandy Vogel
Wieland Kiess
Carine Blanchard
Impact of consumption of the human milk oligosaccharides 2′-FL and LNnT on reduction of risk of allergic sensitisation
Food and Agricultural Immunology
breast milk
human milk oligosaccharides
sensitisation
atopic dermatitis
food allergy
infant nutrition
title Impact of consumption of the human milk oligosaccharides 2′-FL and LNnT on reduction of risk of allergic sensitisation
title_full Impact of consumption of the human milk oligosaccharides 2′-FL and LNnT on reduction of risk of allergic sensitisation
title_fullStr Impact of consumption of the human milk oligosaccharides 2′-FL and LNnT on reduction of risk of allergic sensitisation
title_full_unstemmed Impact of consumption of the human milk oligosaccharides 2′-FL and LNnT on reduction of risk of allergic sensitisation
title_short Impact of consumption of the human milk oligosaccharides 2′-FL and LNnT on reduction of risk of allergic sensitisation
title_sort impact of consumption of the human milk oligosaccharides 2 fl and lnnt on reduction of risk of allergic sensitisation
topic breast milk
human milk oligosaccharides
sensitisation
atopic dermatitis
food allergy
infant nutrition
url https://www.tandfonline.com/doi/10.1080/09540105.2023.2301703
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