Using network pharmacology and molecular docking technology, proteomics and experiments were used to verify the effect of Yigu decoction (YGD) on the expression of key genes in osteoporotic mice
Background Yigu decoction (YGD) is a traditional Chinese medicine prescription for the treatment of osteoporosis, although many clinical studies have confirmed its anti-OP effect, but the specific mechanism is still not completely clear.Methods In this study, through the methods of network pharmacol...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Annals of Medicine |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/07853890.2024.2449225 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841560895263604736 |
|---|---|
| author | Kun Yan Rui-Kun Zhang Jia-Xin Wang Hai-Feng Chen Yang Zhang Feng Cheng Yi Jiang Min Wang Ziqi Wu Xiao-Gang Chen Zhi-Neng Chen Gui-Jin Li Xin-Miao Yao |
| author_facet | Kun Yan Rui-Kun Zhang Jia-Xin Wang Hai-Feng Chen Yang Zhang Feng Cheng Yi Jiang Min Wang Ziqi Wu Xiao-Gang Chen Zhi-Neng Chen Gui-Jin Li Xin-Miao Yao |
| author_sort | Kun Yan |
| collection | DOAJ |
| description | Background Yigu decoction (YGD) is a traditional Chinese medicine prescription for the treatment of osteoporosis, although many clinical studies have confirmed its anti-OP effect, but the specific mechanism is still not completely clear.Methods In this study, through the methods of network pharmacology and molecular docking, the material basis and action target of YGD in preventing and treating OP were analyzed, and the potential target and mechanism of YGD in preventing and treating OP were clarified by TMT quantitative protein and experiment.Results Network pharmacology and molecular docking revealed that the active components of YGD were mainly stigmasterol and flavonoids. Molecular docking mainly studied the strong binding ability of stigmasterol to the target. Animal proteomics verified the related mechanism of YGD in preventing and treating OP. Based on the KEGG enrichment of network pharmacology and histology, our animal experiments in vivo verified that YGD may play a role in the treatment of OP by mediating hif1- α/vegf/glut1 signal pathway.Conclusions YGD prevention and treatment of OP may be achieved by interfering with multiple targets. This study confirmed that it may promote osteoblast proliferation and protect osteoblast function by up-regulating the expression of proteins related to HIF signal pathway. |
| format | Article |
| id | doaj-art-662d61fc0780404880ef24bf4ac7b17c |
| institution | Kabale University |
| issn | 0785-3890 1365-2060 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Annals of Medicine |
| spelling | doaj-art-662d61fc0780404880ef24bf4ac7b17c2025-01-03T10:44:24ZengTaylor & Francis GroupAnnals of Medicine0785-38901365-20602025-12-0157110.1080/07853890.2024.2449225Using network pharmacology and molecular docking technology, proteomics and experiments were used to verify the effect of Yigu decoction (YGD) on the expression of key genes in osteoporotic miceKun Yan0Rui-Kun Zhang1Jia-Xin Wang2Hai-Feng Chen3Yang Zhang4Feng Cheng5Yi Jiang6Min Wang7Ziqi Wu8Xiao-Gang Chen9Zhi-Neng Chen10Gui-Jin Li11Xin-Miao Yao12The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaBackground Yigu decoction (YGD) is a traditional Chinese medicine prescription for the treatment of osteoporosis, although many clinical studies have confirmed its anti-OP effect, but the specific mechanism is still not completely clear.Methods In this study, through the methods of network pharmacology and molecular docking, the material basis and action target of YGD in preventing and treating OP were analyzed, and the potential target and mechanism of YGD in preventing and treating OP were clarified by TMT quantitative protein and experiment.Results Network pharmacology and molecular docking revealed that the active components of YGD were mainly stigmasterol and flavonoids. Molecular docking mainly studied the strong binding ability of stigmasterol to the target. Animal proteomics verified the related mechanism of YGD in preventing and treating OP. Based on the KEGG enrichment of network pharmacology and histology, our animal experiments in vivo verified that YGD may play a role in the treatment of OP by mediating hif1- α/vegf/glut1 signal pathway.Conclusions YGD prevention and treatment of OP may be achieved by interfering with multiple targets. This study confirmed that it may promote osteoblast proliferation and protect osteoblast function by up-regulating the expression of proteins related to HIF signal pathway.https://www.tandfonline.com/doi/10.1080/07853890.2024.2449225OsteoporosisYigu decoctionnetwork pharmacologymolecular dockingbioinformaticsmolecular mechanism |
| spellingShingle | Kun Yan Rui-Kun Zhang Jia-Xin Wang Hai-Feng Chen Yang Zhang Feng Cheng Yi Jiang Min Wang Ziqi Wu Xiao-Gang Chen Zhi-Neng Chen Gui-Jin Li Xin-Miao Yao Using network pharmacology and molecular docking technology, proteomics and experiments were used to verify the effect of Yigu decoction (YGD) on the expression of key genes in osteoporotic mice Annals of Medicine Osteoporosis Yigu decoction network pharmacology molecular docking bioinformatics molecular mechanism |
| title | Using network pharmacology and molecular docking technology, proteomics and experiments were used to verify the effect of Yigu decoction (YGD) on the expression of key genes in osteoporotic mice |
| title_full | Using network pharmacology and molecular docking technology, proteomics and experiments were used to verify the effect of Yigu decoction (YGD) on the expression of key genes in osteoporotic mice |
| title_fullStr | Using network pharmacology and molecular docking technology, proteomics and experiments were used to verify the effect of Yigu decoction (YGD) on the expression of key genes in osteoporotic mice |
| title_full_unstemmed | Using network pharmacology and molecular docking technology, proteomics and experiments were used to verify the effect of Yigu decoction (YGD) on the expression of key genes in osteoporotic mice |
| title_short | Using network pharmacology and molecular docking technology, proteomics and experiments were used to verify the effect of Yigu decoction (YGD) on the expression of key genes in osteoporotic mice |
| title_sort | using network pharmacology and molecular docking technology proteomics and experiments were used to verify the effect of yigu decoction ygd on the expression of key genes in osteoporotic mice |
| topic | Osteoporosis Yigu decoction network pharmacology molecular docking bioinformatics molecular mechanism |
| url | https://www.tandfonline.com/doi/10.1080/07853890.2024.2449225 |
| work_keys_str_mv | AT kunyan usingnetworkpharmacologyandmoleculardockingtechnologyproteomicsandexperimentswereusedtoverifytheeffectofyigudecoctionygdontheexpressionofkeygenesinosteoporoticmice AT ruikunzhang usingnetworkpharmacologyandmoleculardockingtechnologyproteomicsandexperimentswereusedtoverifytheeffectofyigudecoctionygdontheexpressionofkeygenesinosteoporoticmice AT jiaxinwang usingnetworkpharmacologyandmoleculardockingtechnologyproteomicsandexperimentswereusedtoverifytheeffectofyigudecoctionygdontheexpressionofkeygenesinosteoporoticmice AT haifengchen usingnetworkpharmacologyandmoleculardockingtechnologyproteomicsandexperimentswereusedtoverifytheeffectofyigudecoctionygdontheexpressionofkeygenesinosteoporoticmice AT yangzhang usingnetworkpharmacologyandmoleculardockingtechnologyproteomicsandexperimentswereusedtoverifytheeffectofyigudecoctionygdontheexpressionofkeygenesinosteoporoticmice AT fengcheng usingnetworkpharmacologyandmoleculardockingtechnologyproteomicsandexperimentswereusedtoverifytheeffectofyigudecoctionygdontheexpressionofkeygenesinosteoporoticmice AT yijiang usingnetworkpharmacologyandmoleculardockingtechnologyproteomicsandexperimentswereusedtoverifytheeffectofyigudecoctionygdontheexpressionofkeygenesinosteoporoticmice AT minwang usingnetworkpharmacologyandmoleculardockingtechnologyproteomicsandexperimentswereusedtoverifytheeffectofyigudecoctionygdontheexpressionofkeygenesinosteoporoticmice AT ziqiwu usingnetworkpharmacologyandmoleculardockingtechnologyproteomicsandexperimentswereusedtoverifytheeffectofyigudecoctionygdontheexpressionofkeygenesinosteoporoticmice AT xiaogangchen usingnetworkpharmacologyandmoleculardockingtechnologyproteomicsandexperimentswereusedtoverifytheeffectofyigudecoctionygdontheexpressionofkeygenesinosteoporoticmice AT zhinengchen usingnetworkpharmacologyandmoleculardockingtechnologyproteomicsandexperimentswereusedtoverifytheeffectofyigudecoctionygdontheexpressionofkeygenesinosteoporoticmice AT guijinli usingnetworkpharmacologyandmoleculardockingtechnologyproteomicsandexperimentswereusedtoverifytheeffectofyigudecoctionygdontheexpressionofkeygenesinosteoporoticmice AT xinmiaoyao usingnetworkpharmacologyandmoleculardockingtechnologyproteomicsandexperimentswereusedtoverifytheeffectofyigudecoctionygdontheexpressionofkeygenesinosteoporoticmice |