Frequency and antibiotic resistance pattern of Salmonella spp. isolated from traditional dairies and raw milks collected in Yazd province, Iran
Background and Objectives: Salmonellosis is among the most common food-born infections, caused by Salmonella spp. bacteria. Present study has investigated the frequency and antibiotic resistance pattern of Salmonella spp. isolated from traditional dairy products and raw milk supplied in Yazd, Iran....
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Tehran University of Medical Sciences
2024-12-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4679 |
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| author | Raziyeh Barzegar-Bafrouei Bahador Hajimohammadi Hengameh Zandi Gilda Eslami Hossein Fallahzadeh |
| author_facet | Raziyeh Barzegar-Bafrouei Bahador Hajimohammadi Hengameh Zandi Gilda Eslami Hossein Fallahzadeh |
| author_sort | Raziyeh Barzegar-Bafrouei |
| collection | DOAJ |
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Background and Objectives: Salmonellosis is among the most common food-born infections, caused by Salmonella spp. bacteria. Present study has investigated the frequency and antibiotic resistance pattern of Salmonella spp. isolated from traditional dairy products and raw milk supplied in Yazd, Iran.
Materials and Methods: In a cross-sectional study, 350 samples of raw milk and traditional dairy products were randomly collected from July to September 2018. Following culturing the samples, isolates went through biochemical tests for phenotypic identification. Results were confirmed through PCR technique by targeting invA gene. Antimicrobial susceptibility test was conducted by means of disk diffusion method.
Results: The rate of contamination with Salmonella bacteria was 6.57% in all samples. The PCR assay of all isolates showed that 23 isolates (100%) carried the invA gene. No significant association between the frequency of salmonella spp. and types of dairy and their origin was reported (P>0.05). The highest antibiotic resistance rate among the isolates belonged to tetracycline (34.8%) and the highest sensitivity was seen to imipenem, cefepime, and cefotaxime (each 91.3%).
Conclusion: According to our results there has been a rise in multiple drug resistance and contamination rate in traditional dairy products in Yazd province.
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| format | Article |
| id | doaj-art-65ff57e0626041b4862ca808bc17597f |
| institution | Kabale University |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-65ff57e0626041b4862ca808bc17597f2025-01-06T08:38:47ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472024-12-0116610.18502/ijm.v16i6.17248Frequency and antibiotic resistance pattern of Salmonella spp. isolated from traditional dairies and raw milks collected in Yazd province, IranRaziyeh Barzegar-Bafrouei0Bahador Hajimohammadi1Hengameh Zandi2Gilda Eslami3Hossein Fallahzadeh4Department of Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Nutrition, Faculty of Health, Golestan University of Medical Sciences, Gorgan, IranDepartment of Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Medical Parasitology and Mycology, Isfahan Medical School, Isfahan University of Medical Sciences, Isfahan, IranDepartments of Biostatistics and Epidemiology, Research Center for Healthcare Data Modeling, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Background and Objectives: Salmonellosis is among the most common food-born infections, caused by Salmonella spp. bacteria. Present study has investigated the frequency and antibiotic resistance pattern of Salmonella spp. isolated from traditional dairy products and raw milk supplied in Yazd, Iran. Materials and Methods: In a cross-sectional study, 350 samples of raw milk and traditional dairy products were randomly collected from July to September 2018. Following culturing the samples, isolates went through biochemical tests for phenotypic identification. Results were confirmed through PCR technique by targeting invA gene. Antimicrobial susceptibility test was conducted by means of disk diffusion method. Results: The rate of contamination with Salmonella bacteria was 6.57% in all samples. The PCR assay of all isolates showed that 23 isolates (100%) carried the invA gene. No significant association between the frequency of salmonella spp. and types of dairy and their origin was reported (P>0.05). The highest antibiotic resistance rate among the isolates belonged to tetracycline (34.8%) and the highest sensitivity was seen to imipenem, cefepime, and cefotaxime (each 91.3%). Conclusion: According to our results there has been a rise in multiple drug resistance and contamination rate in traditional dairy products in Yazd province. https://ijm.tums.ac.ir/index.php/ijm/article/view/4679Dairy products;invA;Cow’s milk;Salmonella;Antimicrobial resistance |
| spellingShingle | Raziyeh Barzegar-Bafrouei Bahador Hajimohammadi Hengameh Zandi Gilda Eslami Hossein Fallahzadeh Frequency and antibiotic resistance pattern of Salmonella spp. isolated from traditional dairies and raw milks collected in Yazd province, Iran Iranian Journal of Microbiology Dairy products; invA; Cow’s milk; Salmonella; Antimicrobial resistance |
| title | Frequency and antibiotic resistance pattern of Salmonella spp. isolated from traditional dairies and raw milks collected in Yazd province, Iran |
| title_full | Frequency and antibiotic resistance pattern of Salmonella spp. isolated from traditional dairies and raw milks collected in Yazd province, Iran |
| title_fullStr | Frequency and antibiotic resistance pattern of Salmonella spp. isolated from traditional dairies and raw milks collected in Yazd province, Iran |
| title_full_unstemmed | Frequency and antibiotic resistance pattern of Salmonella spp. isolated from traditional dairies and raw milks collected in Yazd province, Iran |
| title_short | Frequency and antibiotic resistance pattern of Salmonella spp. isolated from traditional dairies and raw milks collected in Yazd province, Iran |
| title_sort | frequency and antibiotic resistance pattern of salmonella spp isolated from traditional dairies and raw milks collected in yazd province iran |
| topic | Dairy products; invA; Cow’s milk; Salmonella; Antimicrobial resistance |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4679 |
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