Genetic Diversity Analysis and Polyploid Induction Identification of <i>Idesia polycarpa</i>
<i>Idesia polycarpa</i> from Sichuan is a valuable germplasm with high economic potential, but it faces variety scarcity. To address this, this study collected 16 varieties (lines), identifying IpHT1 as a promising parent due to its high oil content (38.5%) and red fruits. Polyploid indu...
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2024-12-01
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| author | Xiaomei Luo Yunke Liu Yuting Lei Zhoujian He Xiao Gong Meng Ye Qiangang Xiao |
| author_facet | Xiaomei Luo Yunke Liu Yuting Lei Zhoujian He Xiao Gong Meng Ye Qiangang Xiao |
| author_sort | Xiaomei Luo |
| collection | DOAJ |
| description | <i>Idesia polycarpa</i> from Sichuan is a valuable germplasm with high economic potential, but it faces variety scarcity. To address this, this study collected 16 varieties (lines), identifying IpHT1 as a promising parent due to its high oil content (38.5%) and red fruits. Polyploid induction via adding 0.50% colchicine to Murashige and Skoog (MS) medium yielded 520 IpHT1 mutagenized seedlings. Subsequently, flow cytometry (FCM) was performed on 401 morphologically variant seedlings which had been initially screened, resulting in the identification of 15 suspected triploids, 35 suspected tetraploids, and 3 chimeras. Furthermore, fluorescence in situ hybridization (FISH) analysis found that the probe (AG<sub>3</sub>T<sub>3</sub>)<sub>3</sub> had terminal signals at both ends of each chromosome, allowing for the counting of 42 chromosomes in diploids and 84 in tetraploids. The probe 5S rDNA showed 2, 3, and 4 hybridization signals in the interphase nuclei of diploid, triploid, and tetraploid cells, respectively, but the probe (GAA)<sub>6</sub> failed to produce any signal on <i>I. polycarpa</i> chromosomes. Ultimately, 18 polyploids were selected, including 7 triploids and 11 tetraploids. Triploids and tetraploids showed significant leaf morphological and physiological differences from diploids. Consequently, this study successfully established a polyploid breeding system for <i>I. polycarpa</i>, thereby enhancing its genetic diversity and breeding potential. |
| format | Article |
| id | doaj-art-65d0ef2bd45b425eb91d74097ecbc4e2 |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-65d0ef2bd45b425eb91d74097ecbc4e22024-12-13T16:30:15ZengMDPI AGPlants2223-77472024-12-011323339410.3390/plants13233394Genetic Diversity Analysis and Polyploid Induction Identification of <i>Idesia polycarpa</i>Xiaomei Luo0Yunke Liu1Yuting Lei2Zhoujian He3Xiao Gong4Meng Ye5Qiangang Xiao6National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River, College of Forestry, Sichuan Agricultural University, Chengdu 611130, ChinaChengdu Academy of Agriculture and Forestry Sciences, Nongke Road 200, Wenjiang District, Chengdu 611130, ChinaNational Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River, College of Forestry, Sichuan Agricultural University, Chengdu 611130, ChinaNational Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River, College of Forestry, Sichuan Agricultural University, Chengdu 611130, ChinaNational Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River, College of Forestry, Sichuan Agricultural University, Chengdu 611130, ChinaNational Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River, College of Forestry, Sichuan Agricultural University, Chengdu 611130, ChinaChengdu Academy of Agriculture and Forestry Sciences, Nongke Road 200, Wenjiang District, Chengdu 611130, China<i>Idesia polycarpa</i> from Sichuan is a valuable germplasm with high economic potential, but it faces variety scarcity. To address this, this study collected 16 varieties (lines), identifying IpHT1 as a promising parent due to its high oil content (38.5%) and red fruits. Polyploid induction via adding 0.50% colchicine to Murashige and Skoog (MS) medium yielded 520 IpHT1 mutagenized seedlings. Subsequently, flow cytometry (FCM) was performed on 401 morphologically variant seedlings which had been initially screened, resulting in the identification of 15 suspected triploids, 35 suspected tetraploids, and 3 chimeras. Furthermore, fluorescence in situ hybridization (FISH) analysis found that the probe (AG<sub>3</sub>T<sub>3</sub>)<sub>3</sub> had terminal signals at both ends of each chromosome, allowing for the counting of 42 chromosomes in diploids and 84 in tetraploids. The probe 5S rDNA showed 2, 3, and 4 hybridization signals in the interphase nuclei of diploid, triploid, and tetraploid cells, respectively, but the probe (GAA)<sub>6</sub> failed to produce any signal on <i>I. polycarpa</i> chromosomes. Ultimately, 18 polyploids were selected, including 7 triploids and 11 tetraploids. Triploids and tetraploids showed significant leaf morphological and physiological differences from diploids. Consequently, this study successfully established a polyploid breeding system for <i>I. polycarpa</i>, thereby enhancing its genetic diversity and breeding potential.https://www.mdpi.com/2223-7747/13/23/3394genetic diversitybreeding parentpolyploid inductionploidy identification |
| spellingShingle | Xiaomei Luo Yunke Liu Yuting Lei Zhoujian He Xiao Gong Meng Ye Qiangang Xiao Genetic Diversity Analysis and Polyploid Induction Identification of <i>Idesia polycarpa</i> Plants genetic diversity breeding parent polyploid induction ploidy identification |
| title | Genetic Diversity Analysis and Polyploid Induction Identification of <i>Idesia polycarpa</i> |
| title_full | Genetic Diversity Analysis and Polyploid Induction Identification of <i>Idesia polycarpa</i> |
| title_fullStr | Genetic Diversity Analysis and Polyploid Induction Identification of <i>Idesia polycarpa</i> |
| title_full_unstemmed | Genetic Diversity Analysis and Polyploid Induction Identification of <i>Idesia polycarpa</i> |
| title_short | Genetic Diversity Analysis and Polyploid Induction Identification of <i>Idesia polycarpa</i> |
| title_sort | genetic diversity analysis and polyploid induction identification of i idesia polycarpa i |
| topic | genetic diversity breeding parent polyploid induction ploidy identification |
| url | https://www.mdpi.com/2223-7747/13/23/3394 |
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