Longitudinal profiles of oral microbiome in patients with nasopharyngeal carcinoma and their prognostic implications
Background Oral microbiome has been associated with various cancers, including nasopharyngeal carcinoma (NPC), but its role in cancer treatment and prognosis remains largely unknown. This study aims to address the dynamic changes in oral microbiome following cancer treatment and their prognostic imp...
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Taylor & Francis Group
2025-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2024.2447770 |
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| author | Ting Zhou Yan-Xia Wu Xiao-Hui Zheng Xi-Zhao Li Wen-Qiong Xue Tong-Min Wang Yong-Qiao He Shi-Hao Zhou Yan Du Jin-Ru Xie Yi-Wei Chen Li-Xia Lu Ying Liao Wei-Hua Jia |
| author_facet | Ting Zhou Yan-Xia Wu Xiao-Hui Zheng Xi-Zhao Li Wen-Qiong Xue Tong-Min Wang Yong-Qiao He Shi-Hao Zhou Yan Du Jin-Ru Xie Yi-Wei Chen Li-Xia Lu Ying Liao Wei-Hua Jia |
| author_sort | Ting Zhou |
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| description | Background Oral microbiome has been associated with various cancers, including nasopharyngeal carcinoma (NPC), but its role in cancer treatment and prognosis remains largely unknown. This study aims to address the dynamic changes in oral microbiome following cancer treatment and their prognostic implications in NPC patients.Patients and Methods Unstimulated whole saliva samples were collected from 23 NPC patients before and after treatment, with an average of 2.8 samples per patient, and post-treatment saliva samples were collected from additional 13 NPC patients that enrolled after treatment. Following DNA extraction and purification, the salivary microbiome was assessed by 16S rRNA gene amplicon sequencing targeting the V4 hypervariable region.Results Alpha-diversity of oral microbiome decreased progressively after treatment and during follow-up, and the beta-diversity of post-treatment samples differed significantly from the pre-treatment ones (R2 = 0.032, p < 0.001). Among patients free of disease progression, 31 oral taxa were identified that changed significantly in abundances after treatment, with 8 increasing and 23 decreasing. The declining taxa included two previously reported NPC-enriched bacteria, Lautropia mirabilis and Capnocytophaga sputigena. In contrast, in the only recurrent case, the abundances of the two bacteria did not decrease, but remained at high levels or even increased until recurrence occurred.Conclusion NPC treatment can cause persistent decline in microbial diversity of salivary microbiome and abundances of NPC-associated bacteria, and candidate bacteria could be an explanatory factor for NPC prognosis and deserve intensive research. |
| format | Article |
| id | doaj-art-65cc2b9ebd54493da0d65f0862c3b6c2 |
| institution | Kabale University |
| issn | 2000-2297 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj-art-65cc2b9ebd54493da0d65f0862c3b6c22025-01-03T20:26:07ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972025-12-0117110.1080/20002297.2024.2447770Longitudinal profiles of oral microbiome in patients with nasopharyngeal carcinoma and their prognostic implicationsTing Zhou0Yan-Xia Wu1Xiao-Hui Zheng2Xi-Zhao Li3Wen-Qiong Xue4Tong-Min Wang5Yong-Qiao He6Shi-Hao Zhou7Yan Du8Jin-Ru Xie9Yi-Wei Chen10Li-Xia Lu11Ying Liao12Wei-Hua Jia13State Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, ChinaSchool of Public Health, Sun Yat-sen University, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, ChinaBackground Oral microbiome has been associated with various cancers, including nasopharyngeal carcinoma (NPC), but its role in cancer treatment and prognosis remains largely unknown. This study aims to address the dynamic changes in oral microbiome following cancer treatment and their prognostic implications in NPC patients.Patients and Methods Unstimulated whole saliva samples were collected from 23 NPC patients before and after treatment, with an average of 2.8 samples per patient, and post-treatment saliva samples were collected from additional 13 NPC patients that enrolled after treatment. Following DNA extraction and purification, the salivary microbiome was assessed by 16S rRNA gene amplicon sequencing targeting the V4 hypervariable region.Results Alpha-diversity of oral microbiome decreased progressively after treatment and during follow-up, and the beta-diversity of post-treatment samples differed significantly from the pre-treatment ones (R2 = 0.032, p < 0.001). Among patients free of disease progression, 31 oral taxa were identified that changed significantly in abundances after treatment, with 8 increasing and 23 decreasing. The declining taxa included two previously reported NPC-enriched bacteria, Lautropia mirabilis and Capnocytophaga sputigena. In contrast, in the only recurrent case, the abundances of the two bacteria did not decrease, but remained at high levels or even increased until recurrence occurred.Conclusion NPC treatment can cause persistent decline in microbial diversity of salivary microbiome and abundances of NPC-associated bacteria, and candidate bacteria could be an explanatory factor for NPC prognosis and deserve intensive research.https://www.tandfonline.com/doi/10.1080/20002297.2024.2447770Oral microbiomenasopharyngeal carcinomasaliva16S rRNA sequencingprognosis |
| spellingShingle | Ting Zhou Yan-Xia Wu Xiao-Hui Zheng Xi-Zhao Li Wen-Qiong Xue Tong-Min Wang Yong-Qiao He Shi-Hao Zhou Yan Du Jin-Ru Xie Yi-Wei Chen Li-Xia Lu Ying Liao Wei-Hua Jia Longitudinal profiles of oral microbiome in patients with nasopharyngeal carcinoma and their prognostic implications Journal of Oral Microbiology Oral microbiome nasopharyngeal carcinoma saliva 16S rRNA sequencing prognosis |
| title | Longitudinal profiles of oral microbiome in patients with nasopharyngeal carcinoma and their prognostic implications |
| title_full | Longitudinal profiles of oral microbiome in patients with nasopharyngeal carcinoma and their prognostic implications |
| title_fullStr | Longitudinal profiles of oral microbiome in patients with nasopharyngeal carcinoma and their prognostic implications |
| title_full_unstemmed | Longitudinal profiles of oral microbiome in patients with nasopharyngeal carcinoma and their prognostic implications |
| title_short | Longitudinal profiles of oral microbiome in patients with nasopharyngeal carcinoma and their prognostic implications |
| title_sort | longitudinal profiles of oral microbiome in patients with nasopharyngeal carcinoma and their prognostic implications |
| topic | Oral microbiome nasopharyngeal carcinoma saliva 16S rRNA sequencing prognosis |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2024.2447770 |
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