Differential regulation of apoptosis-related genes during long-term culture and differentiation of canine adipose-derived stem cells - a functional bioinformatical analysis
IntroductionStem cells derived from adipose tissue are gaining popularity in the field of regenerative medicine due to their adaptability and clinical potential. Their rapid growth, ability to differentiate, and easy extraction with minimal complications make adipose-derived stem cells (ADSCs) a pro...
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| Format: | Article |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2024.1515778/full |
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| author | Maurycy Jankowski Maurycy Jankowski Maurycy Jankowski Katarzyna Stefańska Michał Suchodolski Claudia Dompe Claudia Dompe Grzegorz Wąsiatycz Bartosz Kempisty Bartosz Kempisty Bartosz Kempisty Bartosz Kempisty Michał Nowicki Magdalena Roszak |
| author_facet | Maurycy Jankowski Maurycy Jankowski Maurycy Jankowski Katarzyna Stefańska Michał Suchodolski Claudia Dompe Claudia Dompe Grzegorz Wąsiatycz Bartosz Kempisty Bartosz Kempisty Bartosz Kempisty Bartosz Kempisty Michał Nowicki Magdalena Roszak |
| author_sort | Maurycy Jankowski |
| collection | DOAJ |
| description | IntroductionStem cells derived from adipose tissue are gaining popularity in the field of regenerative medicine due to their adaptability and clinical potential. Their rapid growth, ability to differentiate, and easy extraction with minimal complications make adipose-derived stem cells (ADSCs) a promising option for many treatments, particularly those targeting bone-related diseases. This study analyzed gene expression in canine ADSCs subjected to long-term culture and osteogenic differentiation.MethodsADSCs were isolated from discarded surgical waste and cultured for 14 days with and without differentiation media to assess osteogenic changes. RNA sequencing (RNA-seq) and bioinformatical analysis were performed to obtain comprehensive transcriptomic data. A total of 17793 genes were detected and GO enrichment analysis was performed on the differentially expressed genes to identify significantly up- and downregulated Biological Process (BP) GO terms across each comparison.ResultsThe upregulation of apoptosis-regulating genes and genes related to circulatory system development suggest an induction of these processes, while the downregulation of neurogenesis and gliogenesis genes points to reciprocal regulation during osteogenic differentiation of canine ADSCs.DiscussionThese findings underscore the potential of ADSCs in bone regeneration and offer valuable insights for advancing tissue engineering, however further studies, including proteomic analyses, are needed to confirm these patterns and their biological significance. |
| format | Article |
| id | doaj-art-6590dc3674d74904be5acb8ab4a9af25 |
| institution | Kabale University |
| issn | 1664-8021 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj-art-6590dc3674d74904be5acb8ab4a9af252025-01-06T06:59:40ZengFrontiers Media S.A.Frontiers in Genetics1664-80212025-01-011510.3389/fgene.2024.15157781515778Differential regulation of apoptosis-related genes during long-term culture and differentiation of canine adipose-derived stem cells - a functional bioinformatical analysisMaurycy Jankowski0Maurycy Jankowski1Maurycy Jankowski2Katarzyna Stefańska3Michał Suchodolski4Claudia Dompe5Claudia Dompe6Grzegorz Wąsiatycz7Bartosz Kempisty8Bartosz Kempisty9Bartosz Kempisty10Bartosz Kempisty11Michał Nowicki12Magdalena Roszak13Department of Computer Science and Statistics, Poznan University of Medical Sciences, Poznan, PolandDeparment of Histology and Embryology, Poznan University of Medical Sciences, Poznan, PolandGreater Poland Center of Digital Medicine, Poznan University of Medical Sciences, Poznan, PolandDepartment of Computer Science and Statistics, Poznan University of Medical Sciences, Poznan, PolandGreater Poland Center of Digital Medicine, Poznan University of Medical Sciences, Poznan, PolandDepartment of Immunology, Poznan University of Medical Sciences, Poznan, PolandDoctoral School, Poznan University of Medical Sciences, Poznan, PolandDepartment of Human Morphology and Embryology, Division of Anatomy, Faculty of Medicine, Wrocław Medical University, Wrocław, PolandDepartment of Human Morphology and Embryology, Division of Anatomy, Faculty of Medicine, Wrocław Medical University, Wrocław, PolandInstitute of Veterinary Medicine, Nicolaus Copernicus University, Torun, PolandPhysiology Graduate Faculty, North Carolina State University, Raleigh, NC, United StatesCenter of Assisted Reproduction, Department of Obstetrics and Gynecology, University Hospital and Masaryk University, Brno, CzechiaDeparment of Histology and Embryology, Poznan University of Medical Sciences, Poznan, PolandDepartment of Computer Science and Statistics, Poznan University of Medical Sciences, Poznan, PolandIntroductionStem cells derived from adipose tissue are gaining popularity in the field of regenerative medicine due to their adaptability and clinical potential. Their rapid growth, ability to differentiate, and easy extraction with minimal complications make adipose-derived stem cells (ADSCs) a promising option for many treatments, particularly those targeting bone-related diseases. This study analyzed gene expression in canine ADSCs subjected to long-term culture and osteogenic differentiation.MethodsADSCs were isolated from discarded surgical waste and cultured for 14 days with and without differentiation media to assess osteogenic changes. RNA sequencing (RNA-seq) and bioinformatical analysis were performed to obtain comprehensive transcriptomic data. A total of 17793 genes were detected and GO enrichment analysis was performed on the differentially expressed genes to identify significantly up- and downregulated Biological Process (BP) GO terms across each comparison.ResultsThe upregulation of apoptosis-regulating genes and genes related to circulatory system development suggest an induction of these processes, while the downregulation of neurogenesis and gliogenesis genes points to reciprocal regulation during osteogenic differentiation of canine ADSCs.DiscussionThese findings underscore the potential of ADSCs in bone regeneration and offer valuable insights for advancing tissue engineering, however further studies, including proteomic analyses, are needed to confirm these patterns and their biological significance.https://www.frontiersin.org/articles/10.3389/fgene.2024.1515778/fulladipose tissuestem cellstranscriptomicsgene expressiongene ontologyapoptosis |
| spellingShingle | Maurycy Jankowski Maurycy Jankowski Maurycy Jankowski Katarzyna Stefańska Michał Suchodolski Claudia Dompe Claudia Dompe Grzegorz Wąsiatycz Bartosz Kempisty Bartosz Kempisty Bartosz Kempisty Bartosz Kempisty Michał Nowicki Magdalena Roszak Differential regulation of apoptosis-related genes during long-term culture and differentiation of canine adipose-derived stem cells - a functional bioinformatical analysis Frontiers in Genetics adipose tissue stem cells transcriptomics gene expression gene ontology apoptosis |
| title | Differential regulation of apoptosis-related genes during long-term culture and differentiation of canine adipose-derived stem cells - a functional bioinformatical analysis |
| title_full | Differential regulation of apoptosis-related genes during long-term culture and differentiation of canine adipose-derived stem cells - a functional bioinformatical analysis |
| title_fullStr | Differential regulation of apoptosis-related genes during long-term culture and differentiation of canine adipose-derived stem cells - a functional bioinformatical analysis |
| title_full_unstemmed | Differential regulation of apoptosis-related genes during long-term culture and differentiation of canine adipose-derived stem cells - a functional bioinformatical analysis |
| title_short | Differential regulation of apoptosis-related genes during long-term culture and differentiation of canine adipose-derived stem cells - a functional bioinformatical analysis |
| title_sort | differential regulation of apoptosis related genes during long term culture and differentiation of canine adipose derived stem cells a functional bioinformatical analysis |
| topic | adipose tissue stem cells transcriptomics gene expression gene ontology apoptosis |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2024.1515778/full |
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