Overexpression of insulin-like growth factor-2 mRNA-binding protein 1 is associated with periodontal disease
Objective: To investigate the potential role of a novel m6A RNA regulator, Insulin-like Growth Factor-2 mRNA-binding protein 1 (IGF2BP1), in periodontal disease pathogenesis. Materials and methods: Gingival tissue samples from 60 periodontitis patients and 60 healthy individuals were analyzed for IG...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-09-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426824000976 |
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| author | Burra Anand Deepika Jaiganesh Ramamurthy Balachander Kannan Vijayashree Priyadharsini Jayaseelan Paramasivam Arumugam |
| author_facet | Burra Anand Deepika Jaiganesh Ramamurthy Balachander Kannan Vijayashree Priyadharsini Jayaseelan Paramasivam Arumugam |
| author_sort | Burra Anand Deepika |
| collection | DOAJ |
| description | Objective: To investigate the potential role of a novel m6A RNA regulator, Insulin-like Growth Factor-2 mRNA-binding protein 1 (IGF2BP1), in periodontal disease pathogenesis. Materials and methods: Gingival tissue samples from 60 periodontitis patients and 60 healthy individuals were analyzed for IGF2BP1 mRNA and protein expression via real-time quantitative PCR (RT-qPCR) and Western blotting. Additionally, Porphyromonas gingivalis Lipopolysaccharide (Pg-LPS) -induced human gingival fibroblasts (HGFs) were evaluated for IGF2BP1 and proinflammatory cytokine expression. In silico functional analysis further explored potential molecular mechanisms. Results: IGF2BP1 mRNA and protein levels were significantly higher in the periodontitis group compared to the healthy group. Functional analysis implicated IGF2BP1 in regulating the IL-17 signaling pathway, a key player in inflammation. Pg-LPS treatment upregulated IGF2BP1 and proinflammatory cytokines in HGFs, supporting this finding. Conclusion: Our study suggests that IGF2BP1 overexpression contributes to periodontitis pathogenesis, potentially through IL-17 signaling. Further research is needed to elucidate the precise molecular mechanisms and explore IGF2BP1 as a potential therapeutic target or biomarker for this common oral disease. |
| format | Article |
| id | doaj-art-653effa1b6c346a7b85a769513f74818 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-653effa1b6c346a7b85a769513f748182024-11-23T06:31:01ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682024-09-01145494499Overexpression of insulin-like growth factor-2 mRNA-binding protein 1 is associated with periodontal diseaseBurra Anand Deepika0Jaiganesh Ramamurthy1Balachander Kannan2Vijayashree Priyadharsini Jayaseelan3Paramasivam Arumugam4Department of Periodontics, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, IndiaDepartment of Periodontics, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, IndiaMolecular Biology Lab, Centre for Cellular and Molecular Research, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, IndiaClinical Genetics Lab, Centre for Cellular and Molecular Research, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, IndiaMolecular Biology Lab, Centre for Cellular and Molecular Research, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, India; Corresponding author. Centre for Cellular and Molecular Research, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600077, TN, India.Objective: To investigate the potential role of a novel m6A RNA regulator, Insulin-like Growth Factor-2 mRNA-binding protein 1 (IGF2BP1), in periodontal disease pathogenesis. Materials and methods: Gingival tissue samples from 60 periodontitis patients and 60 healthy individuals were analyzed for IGF2BP1 mRNA and protein expression via real-time quantitative PCR (RT-qPCR) and Western blotting. Additionally, Porphyromonas gingivalis Lipopolysaccharide (Pg-LPS) -induced human gingival fibroblasts (HGFs) were evaluated for IGF2BP1 and proinflammatory cytokine expression. In silico functional analysis further explored potential molecular mechanisms. Results: IGF2BP1 mRNA and protein levels were significantly higher in the periodontitis group compared to the healthy group. Functional analysis implicated IGF2BP1 in regulating the IL-17 signaling pathway, a key player in inflammation. Pg-LPS treatment upregulated IGF2BP1 and proinflammatory cytokines in HGFs, supporting this finding. Conclusion: Our study suggests that IGF2BP1 overexpression contributes to periodontitis pathogenesis, potentially through IL-17 signaling. Further research is needed to elucidate the precise molecular mechanisms and explore IGF2BP1 as a potential therapeutic target or biomarker for this common oral disease.http://www.sciencedirect.com/science/article/pii/S2212426824000976HealthPeriodontitisGeneticsNovel m6A readerIGF2BP1 |
| spellingShingle | Burra Anand Deepika Jaiganesh Ramamurthy Balachander Kannan Vijayashree Priyadharsini Jayaseelan Paramasivam Arumugam Overexpression of insulin-like growth factor-2 mRNA-binding protein 1 is associated with periodontal disease Journal of Oral Biology and Craniofacial Research Health Periodontitis Genetics Novel m6A reader IGF2BP1 |
| title | Overexpression of insulin-like growth factor-2 mRNA-binding protein 1 is associated with periodontal disease |
| title_full | Overexpression of insulin-like growth factor-2 mRNA-binding protein 1 is associated with periodontal disease |
| title_fullStr | Overexpression of insulin-like growth factor-2 mRNA-binding protein 1 is associated with periodontal disease |
| title_full_unstemmed | Overexpression of insulin-like growth factor-2 mRNA-binding protein 1 is associated with periodontal disease |
| title_short | Overexpression of insulin-like growth factor-2 mRNA-binding protein 1 is associated with periodontal disease |
| title_sort | overexpression of insulin like growth factor 2 mrna binding protein 1 is associated with periodontal disease |
| topic | Health Periodontitis Genetics Novel m6A reader IGF2BP1 |
| url | http://www.sciencedirect.com/science/article/pii/S2212426824000976 |
| work_keys_str_mv | AT burraananddeepika overexpressionofinsulinlikegrowthfactor2mrnabindingprotein1isassociatedwithperiodontaldisease AT jaiganeshramamurthy overexpressionofinsulinlikegrowthfactor2mrnabindingprotein1isassociatedwithperiodontaldisease AT balachanderkannan overexpressionofinsulinlikegrowthfactor2mrnabindingprotein1isassociatedwithperiodontaldisease AT vijayashreepriyadharsinijayaseelan overexpressionofinsulinlikegrowthfactor2mrnabindingprotein1isassociatedwithperiodontaldisease AT paramasivamarumugam overexpressionofinsulinlikegrowthfactor2mrnabindingprotein1isassociatedwithperiodontaldisease |