Study on the antioxidant and antiosteoporotic activities of the oyster peptides prepared by ultrasound-assisted enzymatic hydrolysis
In this study, the effects of ultrasound-assisted enzymatic hydrolysis on the production of antioxidant and antiosteoporotic peptides derived from oysters were investigated. Results showed that ultrasound-assisted enzymatic hydrolysis significantly enhanced the peptide content, free radical scavengi...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ultrasonics Sonochemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417724004607 |
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| author | Zhengze Quan Zonghan Wang Zixu Wang Zuoxu Hou Bin Liu Xiaoming Guo Beiwei Zhu Yuanyuan Hu |
| author_facet | Zhengze Quan Zonghan Wang Zixu Wang Zuoxu Hou Bin Liu Xiaoming Guo Beiwei Zhu Yuanyuan Hu |
| author_sort | Zhengze Quan |
| collection | DOAJ |
| description | In this study, the effects of ultrasound-assisted enzymatic hydrolysis on the production of antioxidant and antiosteoporotic peptides derived from oysters were investigated. Results showed that ultrasound-assisted enzymatic hydrolysis significantly enhanced the peptide content, free radical scavenging ability, and ferric reducing antioxidant power of total oyster protein hydrolysate (TOPH), with optimal results achieved at 200 W (TOPH-200). Correspondingly, ultrasound treatment at 200 W increased the exposure of hydrophobic regions, reduced α-helix content, and facilitated the generation of small molecular weight peptides in TOPH. In an H2O2-induced oxidative damage model of osteoblastic MC3T3-E1 cells, TOPH-200 significantly attenuated intracellular reactive oxygen species and improved mitochondrial membrane potential. Importantly, TOPH-200 effectively enhanced osteogenic cell proliferation, differentiation, and mineralization in H2O2-treated MC3T3-E1 cells. Additionally, two novel peptides, DSQLAPFRF and HFNPRL, were screened from the TOPH-200 using PeptideRanker and molecular docking. Further cell experiments indicated that both peptides exhibited potent antioxidant and antiosteoporotic activities in oxidatively damaged MC3T3-E1 cells. In summary, mild ultrasound-assisted enzymatic hydrolysis proved effective in producing bioactive peptides from oysters, and these newly identified peptides exhibit potential for osteoporosis prevention. |
| format | Article |
| id | doaj-art-64a981f40ebb4b35a421cfb77b9a0823 |
| institution | Kabale University |
| issn | 1350-4177 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-64a981f40ebb4b35a421cfb77b9a08232025-01-11T06:38:51ZengElsevierUltrasonics Sonochemistry1350-41772025-01-01112107211Study on the antioxidant and antiosteoporotic activities of the oyster peptides prepared by ultrasound-assisted enzymatic hydrolysisZhengze Quan0Zonghan Wang1Zixu Wang2Zuoxu Hou3Bin Liu4Xiaoming Guo5Beiwei Zhu6Yuanyuan Hu7Shenzhen Key Laboratory of Food Nutrition and Health, Guangdong Engineering Technology Research Center of Aquatic Food Processing and Safety Control, School of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, ChinaShenzhen Key Laboratory of Food Nutrition and Health, Guangdong Engineering Technology Research Center of Aquatic Food Processing and Safety Control, School of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, ChinaState Key Laboratory of Marine Food Processing & Safety Control, National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaDepartment of Orthopedics, Shenzhen Hospital, Southern Medical University, Shenzhen 518101, ChinaShenzhen Key Laboratory of Food Nutrition and Health, Guangdong Engineering Technology Research Center of Aquatic Food Processing and Safety Control, School of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, ChinaShenzhen Key Laboratory of Food Nutrition and Health, Guangdong Engineering Technology Research Center of Aquatic Food Processing and Safety Control, School of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, ChinaShenzhen Key Laboratory of Food Nutrition and Health, Guangdong Engineering Technology Research Center of Aquatic Food Processing and Safety Control, School of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, China; State Key Laboratory of Marine Food Processing & Safety Control, National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; Corresponding authors.Shenzhen Key Laboratory of Food Nutrition and Health, Guangdong Engineering Technology Research Center of Aquatic Food Processing and Safety Control, School of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, China; Corresponding authors.In this study, the effects of ultrasound-assisted enzymatic hydrolysis on the production of antioxidant and antiosteoporotic peptides derived from oysters were investigated. Results showed that ultrasound-assisted enzymatic hydrolysis significantly enhanced the peptide content, free radical scavenging ability, and ferric reducing antioxidant power of total oyster protein hydrolysate (TOPH), with optimal results achieved at 200 W (TOPH-200). Correspondingly, ultrasound treatment at 200 W increased the exposure of hydrophobic regions, reduced α-helix content, and facilitated the generation of small molecular weight peptides in TOPH. In an H2O2-induced oxidative damage model of osteoblastic MC3T3-E1 cells, TOPH-200 significantly attenuated intracellular reactive oxygen species and improved mitochondrial membrane potential. Importantly, TOPH-200 effectively enhanced osteogenic cell proliferation, differentiation, and mineralization in H2O2-treated MC3T3-E1 cells. Additionally, two novel peptides, DSQLAPFRF and HFNPRL, were screened from the TOPH-200 using PeptideRanker and molecular docking. Further cell experiments indicated that both peptides exhibited potent antioxidant and antiosteoporotic activities in oxidatively damaged MC3T3-E1 cells. In summary, mild ultrasound-assisted enzymatic hydrolysis proved effective in producing bioactive peptides from oysters, and these newly identified peptides exhibit potential for osteoporosis prevention.http://www.sciencedirect.com/science/article/pii/S1350417724004607OysterUltrasoundEnzymatic hydrolysisBioactive peptidesAntiosteoporotic activity |
| spellingShingle | Zhengze Quan Zonghan Wang Zixu Wang Zuoxu Hou Bin Liu Xiaoming Guo Beiwei Zhu Yuanyuan Hu Study on the antioxidant and antiosteoporotic activities of the oyster peptides prepared by ultrasound-assisted enzymatic hydrolysis Ultrasonics Sonochemistry Oyster Ultrasound Enzymatic hydrolysis Bioactive peptides Antiosteoporotic activity |
| title | Study on the antioxidant and antiosteoporotic activities of the oyster peptides prepared by ultrasound-assisted enzymatic hydrolysis |
| title_full | Study on the antioxidant and antiosteoporotic activities of the oyster peptides prepared by ultrasound-assisted enzymatic hydrolysis |
| title_fullStr | Study on the antioxidant and antiosteoporotic activities of the oyster peptides prepared by ultrasound-assisted enzymatic hydrolysis |
| title_full_unstemmed | Study on the antioxidant and antiosteoporotic activities of the oyster peptides prepared by ultrasound-assisted enzymatic hydrolysis |
| title_short | Study on the antioxidant and antiosteoporotic activities of the oyster peptides prepared by ultrasound-assisted enzymatic hydrolysis |
| title_sort | study on the antioxidant and antiosteoporotic activities of the oyster peptides prepared by ultrasound assisted enzymatic hydrolysis |
| topic | Oyster Ultrasound Enzymatic hydrolysis Bioactive peptides Antiosteoporotic activity |
| url | http://www.sciencedirect.com/science/article/pii/S1350417724004607 |
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