Dynamic Changes of Gut Microbiome in Borneo Earless Monitor Lizard Across Different Diets
The Borneo earless monitor lizard (Lanthanotus borneensis) is one of the endemic animals of Indonesia. In 2019, the IUCN listed L. borneensis as an ‘endangered’ species. The declining population of the L. borneensis is a major concern for conservation efforts. One of the efforts to conserve endange...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2024-11-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/56743 |
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| author | Milsa Solva Diana Ainissya Fitri Meryandini Anja Roni Ridwan |
| author_facet | Milsa Solva Diana Ainissya Fitri Meryandini Anja Roni Ridwan |
| author_sort | Milsa Solva Diana |
| collection | DOAJ |
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The Borneo earless monitor lizard (Lanthanotus borneensis) is one of the endemic animals of Indonesia. In 2019, the IUCN listed L. borneensis as an ‘endangered’ species. The declining population of the L. borneensis is a major concern for conservation efforts. One of the efforts to conserve endangered wild animals is to know the gut microbiome profile and the relationship between the host and the bacterial community in the body, which is associated with an influence on health. This research aims to determine the gut microbiome profile of animals with metagenomic analysis using Next-Generation Sequencing (NGS). This research was carried out by providing three feed types: earthworms, shrimp, and fish. Sample feces was collected by cloacal swabs and the DNA was sequenced using the Illumina NGS technique in the V4 16S rRNA region to examine the gut microbiome. The results of the metagenomic analysis showed differences in bacterial abundance in each feed treatment. The L. borneensis treated with earthworms and fish were dominated at the phylum level by Proteobacteria and Bacteriodota, while in shrimp, the phyla were Proteobacteria and Firmicutes. Index Shanon, Simpson, and the rarefaction curve also showed the highest bacterial diversity found in the fish treatment. This study can be used as a reference in designing suitable feed formulations for effective captive breeding for the conservation of L. borneensis
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| format | Article |
| id | doaj-art-6480e09b6bbe489ebb828011efb6dd58 |
| institution | Kabale University |
| issn | 1978-3019 2086-4094 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj-art-6480e09b6bbe489ebb828011efb6dd582024-11-25T03:41:18ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942024-11-0132110.4308/hjb.32.1.80-88Dynamic Changes of Gut Microbiome in Borneo Earless Monitor Lizard Across Different DietsMilsa Solva Diana0Ainissya Fitri1Meryandini Anja2Roni Ridwan3Study Program of Microbiology, Graduate School of IPB University, Bogor 16680, IndonesiaResearch Center for Applied Zoology, National Research and Innovation Agency (BRIN), Cibinong 16915, IndonesiaStudy Program of Microbiology, Graduate School of IPB University, Bogor 16680, Indonesia. Department of Biology, Faculty of Mathematics and Natural Science, IPB University, Bogor 16680, IndonesiaResearch Center for Applied Zoology, National Research and Innovation Agency (BRIN), Cibinong 16915, Indonesia The Borneo earless monitor lizard (Lanthanotus borneensis) is one of the endemic animals of Indonesia. In 2019, the IUCN listed L. borneensis as an ‘endangered’ species. The declining population of the L. borneensis is a major concern for conservation efforts. One of the efforts to conserve endangered wild animals is to know the gut microbiome profile and the relationship between the host and the bacterial community in the body, which is associated with an influence on health. This research aims to determine the gut microbiome profile of animals with metagenomic analysis using Next-Generation Sequencing (NGS). This research was carried out by providing three feed types: earthworms, shrimp, and fish. Sample feces was collected by cloacal swabs and the DNA was sequenced using the Illumina NGS technique in the V4 16S rRNA region to examine the gut microbiome. The results of the metagenomic analysis showed differences in bacterial abundance in each feed treatment. The L. borneensis treated with earthworms and fish were dominated at the phylum level by Proteobacteria and Bacteriodota, while in shrimp, the phyla were Proteobacteria and Firmicutes. Index Shanon, Simpson, and the rarefaction curve also showed the highest bacterial diversity found in the fish treatment. This study can be used as a reference in designing suitable feed formulations for effective captive breeding for the conservation of L. borneensis https://journal.ipb.ac.id/index.php/hayati/article/view/56743 |
| spellingShingle | Milsa Solva Diana Ainissya Fitri Meryandini Anja Roni Ridwan Dynamic Changes of Gut Microbiome in Borneo Earless Monitor Lizard Across Different Diets Hayati Journal of Biosciences |
| title | Dynamic Changes of Gut Microbiome in Borneo Earless Monitor Lizard Across Different Diets |
| title_full | Dynamic Changes of Gut Microbiome in Borneo Earless Monitor Lizard Across Different Diets |
| title_fullStr | Dynamic Changes of Gut Microbiome in Borneo Earless Monitor Lizard Across Different Diets |
| title_full_unstemmed | Dynamic Changes of Gut Microbiome in Borneo Earless Monitor Lizard Across Different Diets |
| title_short | Dynamic Changes of Gut Microbiome in Borneo Earless Monitor Lizard Across Different Diets |
| title_sort | dynamic changes of gut microbiome in borneo earless monitor lizard across different diets |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/56743 |
| work_keys_str_mv | AT milsasolvadiana dynamicchangesofgutmicrobiomeinborneoearlessmonitorlizardacrossdifferentdiets AT ainissyafitri dynamicchangesofgutmicrobiomeinborneoearlessmonitorlizardacrossdifferentdiets AT meryandinianja dynamicchangesofgutmicrobiomeinborneoearlessmonitorlizardacrossdifferentdiets AT roniridwan dynamicchangesofgutmicrobiomeinborneoearlessmonitorlizardacrossdifferentdiets |