Trichoderma reesei JCM 2267, fungi-associated sponge Stylissa flabelliformis: Effects of applied different fermentation methods on their antibacterial activity
Trichoderma is one of the fungi producing a wide range of metabolites with potent biological activities, including antibacterial. Selecting appropriate fermentation methods can improve the production of metabolite compounds and provide the best biological activity. This study investigates Trichoderm...
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| Format: | Article |
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Wolters Kluwer Medknow Publications
2024-10-01
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| Series: | Journal of Advanced Pharmaceutical Technology & Research |
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| Online Access: | https://journals.lww.com/japtr/fulltext/2024/15040/trichoderma_reesei_jcm_2267,_fungi_associated.1.aspx |
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| author | Dyan Wigati Erna Prawita Setyowati Prawita Erna Sylvia Utami Tunjung Pratiwi Ari Satia Nugraha |
| author_facet | Dyan Wigati Erna Prawita Setyowati Prawita Erna Sylvia Utami Tunjung Pratiwi Ari Satia Nugraha |
| author_sort | Dyan Wigati |
| collection | DOAJ |
| description | Trichoderma is one of the fungi producing a wide range of metabolites with potent biological activities, including antibacterial. Selecting appropriate fermentation methods can improve the production of metabolite compounds and provide the best biological activity. This study investigates Trichoderma reesei JCM 2267’s antibacterial activity, which is fermented using submerged fermentation (SmF) and solid-state fermentation (SSF). The effect of fermentation time on antibacterial activities and yield weight was also determined. Antibacterial activity was tested against Staphylococcus aureus (S.a) ATCC 25923, Escherichia coli (E.c) ATCC 25922, and Pseudomonas aeruginosa (P.a) ATCC 27853 using the Agar well diffusion method. This research successfully demonstrated that the SmF and SSF methods provided extracts with antibacterial activity ranging from weak to strong, against bacteria tested. The extract fermented by SSF showed the best activity on day 25 of fermentation with a diameter clear zone 12.2 ± 0.20; 5.0 ± 0.17, and 3.3 ± 0.1 (mm) against S.a ATCC 25923, E.c ATCC 25922, and P.a ATCC 27853. The extract fermented by SmF, especially the supernatant extract, reached the higher inhibition on day 8 with diameter inhibition 6.90 ± 0.05 and 8.20 ± 0.04 (mm) against E.c ATCC 25922 and P.a ATCC 27853, whereas 15.3 ± 0.10 (mm) against S.a ATCC 25923 on day 14 of fermentation. The various times of fermentation affect the antibacterial activities. The maximum growth of TR JCM 2267 was reached on day 14 of fermentation. The SSF methods showed a higher yield of extract than SmF, increasing over time of fermentation. This research provides a possible alternative fermentation method suitable for fungal growth related to its antibacterial activity and extract yield. |
| format | Article |
| id | doaj-art-63e9e6e0b1a2452d96a8053be4cf722f |
| institution | Kabale University |
| issn | 0976-2094 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Advanced Pharmaceutical Technology & Research |
| spelling | doaj-art-63e9e6e0b1a2452d96a8053be4cf722f2024-12-15T10:05:15ZengWolters Kluwer Medknow PublicationsJournal of Advanced Pharmaceutical Technology & Research0976-20942024-10-0115424324710.4103/JAPTR.JAPTR_7_24Trichoderma reesei JCM 2267, fungi-associated sponge Stylissa flabelliformis: Effects of applied different fermentation methods on their antibacterial activityDyan WigatiErna Prawita SetyowatiPrawita Erna Sylvia Utami Tunjung PratiwiAri Satia NugrahaTrichoderma is one of the fungi producing a wide range of metabolites with potent biological activities, including antibacterial. Selecting appropriate fermentation methods can improve the production of metabolite compounds and provide the best biological activity. This study investigates Trichoderma reesei JCM 2267’s antibacterial activity, which is fermented using submerged fermentation (SmF) and solid-state fermentation (SSF). The effect of fermentation time on antibacterial activities and yield weight was also determined. Antibacterial activity was tested against Staphylococcus aureus (S.a) ATCC 25923, Escherichia coli (E.c) ATCC 25922, and Pseudomonas aeruginosa (P.a) ATCC 27853 using the Agar well diffusion method. This research successfully demonstrated that the SmF and SSF methods provided extracts with antibacterial activity ranging from weak to strong, against bacteria tested. The extract fermented by SSF showed the best activity on day 25 of fermentation with a diameter clear zone 12.2 ± 0.20; 5.0 ± 0.17, and 3.3 ± 0.1 (mm) against S.a ATCC 25923, E.c ATCC 25922, and P.a ATCC 27853. The extract fermented by SmF, especially the supernatant extract, reached the higher inhibition on day 8 with diameter inhibition 6.90 ± 0.05 and 8.20 ± 0.04 (mm) against E.c ATCC 25922 and P.a ATCC 27853, whereas 15.3 ± 0.10 (mm) against S.a ATCC 25923 on day 14 of fermentation. The various times of fermentation affect the antibacterial activities. The maximum growth of TR JCM 2267 was reached on day 14 of fermentation. The SSF methods showed a higher yield of extract than SmF, increasing over time of fermentation. This research provides a possible alternative fermentation method suitable for fungal growth related to its antibacterial activity and extract yield.https://journals.lww.com/japtr/fulltext/2024/15040/trichoderma_reesei_jcm_2267,_fungi_associated.1.aspxbacterial inhibitor; fermentation methods; fungi -associated; growth curve |
| spellingShingle | Dyan Wigati Erna Prawita Setyowati Prawita Erna Sylvia Utami Tunjung Pratiwi Ari Satia Nugraha Trichoderma reesei JCM 2267, fungi-associated sponge Stylissa flabelliformis: Effects of applied different fermentation methods on their antibacterial activity Journal of Advanced Pharmaceutical Technology & Research bacterial inhibitor; fermentation methods; fungi -associated; growth curve |
| title | Trichoderma reesei JCM 2267, fungi-associated sponge Stylissa flabelliformis: Effects of applied different fermentation methods on their antibacterial activity |
| title_full | Trichoderma reesei JCM 2267, fungi-associated sponge Stylissa flabelliformis: Effects of applied different fermentation methods on their antibacterial activity |
| title_fullStr | Trichoderma reesei JCM 2267, fungi-associated sponge Stylissa flabelliformis: Effects of applied different fermentation methods on their antibacterial activity |
| title_full_unstemmed | Trichoderma reesei JCM 2267, fungi-associated sponge Stylissa flabelliformis: Effects of applied different fermentation methods on their antibacterial activity |
| title_short | Trichoderma reesei JCM 2267, fungi-associated sponge Stylissa flabelliformis: Effects of applied different fermentation methods on their antibacterial activity |
| title_sort | trichoderma reesei jcm 2267 fungi associated sponge stylissa flabelliformis effects of applied different fermentation methods on their antibacterial activity |
| topic | bacterial inhibitor; fermentation methods; fungi -associated; growth curve |
| url | https://journals.lww.com/japtr/fulltext/2024/15040/trichoderma_reesei_jcm_2267,_fungi_associated.1.aspx |
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