Topotecan Incorporation Into Avocado Oil-based Nanoemulsion Potentiates Its Inhibitory Effect on the Cellular Growth of Various Human Cancer Cells
Background: The aim is to ameliorate the anticancer activity of topotecan (topoisomerase [TOPO]) by solubilizing it in a nanoemulsion (NE) delivery system containing avocado oil (AO), then to assess its cytotoxicity in MCF-7 breast, HCT116 colon, HeLa cervical, and HepG2 liver cancer cells. Methods:...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Biomedical and Biotechnology Research Journal |
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| Online Access: | https://journals.lww.com/10.4103/bbrj.bbrj_287_24 |
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| _version_ | 1841554777945669632 |
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| author | Mayson H. Alkhatib Hadeel M. Bayoumi |
| author_facet | Mayson H. Alkhatib Hadeel M. Bayoumi |
| author_sort | Mayson H. Alkhatib |
| collection | DOAJ |
| description | Background:
The aim is to ameliorate the anticancer activity of topotecan (topoisomerase [TOPO]) by solubilizing it in a nanoemulsion (NE) delivery system containing avocado oil (AO), then to assess its cytotoxicity in MCF-7 breast, HCT116 colon, HeLa cervical, and HepG2 liver cancer cells.
Methods:
AO-NE formulation was developed using an ultrasonic homogenizer and characterized by a zeta-sizer and transmission electron microscope (TEM). The effects of the TOPO-AO-NE formula on viability, cellular and nuclear morphology of cancer cells, as well as TOPO cellular accumulation, inflammatory effects, apoptosis, and autophagy, were examined.
Results:
The nanosized particles of the AO-NE formula were negatively charged (114.83 ± 1.10 nm, −8.96 ± 0.14 mV) even after TOPO loading (75.44 ± 8.51 nm,−5.79 ± 0.38 mV). In contrast to free TOPO, TOPO-AO-NE exhibited significant anti-proliferation, clear apoptotic changes, improved TOPO cellular uptake and decreased IC50 from (12.65 ± 0.05, 15.78 ± 0.08, 26.82 ± 0.50, and 5.50 ± 0.50 μM) to (2.35 ± 0.05, 4.90 ± 0.05, 2.58 ± 0.08, and 4.87 ± 0.08 μM) in MCF-7, HCT116, HeLa, and HepG2 cells, respectively. In MCF-7 and HCT116 cells, cytotoxicity of TOPO-AO-NE was attributed to decreased IL-6 levels relative to a negative control, lessened autophagic LC-3 levels compared to free TOPO-treated cells, and induction of type-I apoptotic cell death. In HeLa cells, TOPO-AO-NE-induced apoptosis through autophagy inhibition and induction of type-I apoptosis. In HepG2 cells, AO-NE reduced IL-6 cytokines and induced both type-I-apoptotic and type-II-autophagic cell deaths in comparison with control cells.
Conclusion:
Loading TOPO in AO-NE has dramatically enhanced its’ antiproliferative effects in cancer cells. |
| format | Article |
| id | doaj-art-63de5d20b2be409c983c30c628a41940 |
| institution | Kabale University |
| issn | 2588-9834 2588-9842 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Biomedical and Biotechnology Research Journal |
| spelling | doaj-art-63de5d20b2be409c983c30c628a419402025-01-08T09:51:17ZengWolters Kluwer Medknow PublicationsBiomedical and Biotechnology Research Journal2588-98342588-98422024-12-018447448610.4103/bbrj.bbrj_287_24Topotecan Incorporation Into Avocado Oil-based Nanoemulsion Potentiates Its Inhibitory Effect on the Cellular Growth of Various Human Cancer CellsMayson H. AlkhatibHadeel M. BayoumiBackground: The aim is to ameliorate the anticancer activity of topotecan (topoisomerase [TOPO]) by solubilizing it in a nanoemulsion (NE) delivery system containing avocado oil (AO), then to assess its cytotoxicity in MCF-7 breast, HCT116 colon, HeLa cervical, and HepG2 liver cancer cells. Methods: AO-NE formulation was developed using an ultrasonic homogenizer and characterized by a zeta-sizer and transmission electron microscope (TEM). The effects of the TOPO-AO-NE formula on viability, cellular and nuclear morphology of cancer cells, as well as TOPO cellular accumulation, inflammatory effects, apoptosis, and autophagy, were examined. Results: The nanosized particles of the AO-NE formula were negatively charged (114.83 ± 1.10 nm, −8.96 ± 0.14 mV) even after TOPO loading (75.44 ± 8.51 nm,−5.79 ± 0.38 mV). In contrast to free TOPO, TOPO-AO-NE exhibited significant anti-proliferation, clear apoptotic changes, improved TOPO cellular uptake and decreased IC50 from (12.65 ± 0.05, 15.78 ± 0.08, 26.82 ± 0.50, and 5.50 ± 0.50 μM) to (2.35 ± 0.05, 4.90 ± 0.05, 2.58 ± 0.08, and 4.87 ± 0.08 μM) in MCF-7, HCT116, HeLa, and HepG2 cells, respectively. In MCF-7 and HCT116 cells, cytotoxicity of TOPO-AO-NE was attributed to decreased IL-6 levels relative to a negative control, lessened autophagic LC-3 levels compared to free TOPO-treated cells, and induction of type-I apoptotic cell death. In HeLa cells, TOPO-AO-NE-induced apoptosis through autophagy inhibition and induction of type-I apoptosis. In HepG2 cells, AO-NE reduced IL-6 cytokines and induced both type-I-apoptotic and type-II-autophagic cell deaths in comparison with control cells. Conclusion: Loading TOPO in AO-NE has dramatically enhanced its’ antiproliferative effects in cancer cells.https://journals.lww.com/10.4103/bbrj.bbrj_287_24apoptosisautophagycaspase-3cellular uptakeinterleukin-6 |
| spellingShingle | Mayson H. Alkhatib Hadeel M. Bayoumi Topotecan Incorporation Into Avocado Oil-based Nanoemulsion Potentiates Its Inhibitory Effect on the Cellular Growth of Various Human Cancer Cells Biomedical and Biotechnology Research Journal apoptosis autophagy caspase-3 cellular uptake interleukin-6 |
| title | Topotecan Incorporation Into Avocado Oil-based Nanoemulsion Potentiates Its Inhibitory Effect on the Cellular Growth of Various Human Cancer Cells |
| title_full | Topotecan Incorporation Into Avocado Oil-based Nanoemulsion Potentiates Its Inhibitory Effect on the Cellular Growth of Various Human Cancer Cells |
| title_fullStr | Topotecan Incorporation Into Avocado Oil-based Nanoemulsion Potentiates Its Inhibitory Effect on the Cellular Growth of Various Human Cancer Cells |
| title_full_unstemmed | Topotecan Incorporation Into Avocado Oil-based Nanoemulsion Potentiates Its Inhibitory Effect on the Cellular Growth of Various Human Cancer Cells |
| title_short | Topotecan Incorporation Into Avocado Oil-based Nanoemulsion Potentiates Its Inhibitory Effect on the Cellular Growth of Various Human Cancer Cells |
| title_sort | topotecan incorporation into avocado oil based nanoemulsion potentiates its inhibitory effect on the cellular growth of various human cancer cells |
| topic | apoptosis autophagy caspase-3 cellular uptake interleukin-6 |
| url | https://journals.lww.com/10.4103/bbrj.bbrj_287_24 |
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