Multiplexing ultraviolet-excited ultrasound and autofluorescence enables slide-free and label-free intraoperative histopathology imaging
Histological examination of tissue remains the gold standard for analysis of various diseases in both clinical diagnosis and basic research. However, long-standing challenges in conventional histology have arisen from complex sample preparations involving micrometer-thick sectioning and hematoxylin...
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| Format: | Article |
| Language: | English |
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AIP Publishing LLC
2024-11-01
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| Series: | APL Photonics |
| Online Access: | http://dx.doi.org/10.1063/5.0232343 |
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| author | Wei Song Xiaobao Wang Yiyan Zhuang Yechen Wang Qinlian Ye Ya-chao Wang Xiaocong Yuan |
| author_facet | Wei Song Xiaobao Wang Yiyan Zhuang Yechen Wang Qinlian Ye Ya-chao Wang Xiaocong Yuan |
| author_sort | Wei Song |
| collection | DOAJ |
| description | Histological examination of tissue remains the gold standard for analysis of various diseases in both clinical diagnosis and basic research. However, long-standing challenges in conventional histology have arisen from complex sample preparations involving micrometer-thick sectioning and hematoxylin and eosin (H&E) staining processes, which lead to significant delays in accessing the diagnostic output. We propose a hybrid optical-ultrasonic microscope (HOME) that multiplexes the intrinsic thermoelastic ultrasound and fluorescence emission generated by endogenous biomolecules under single-shot ultraviolet excitation to achieve direct histopathological imaging of fresh tissue without the sectioning and staining steps. The HOME detects ultrasounds of DNA/RNA using a photoacoustic microscopy subsystem to provide a hematoxylin-like contrast that specifically highlights the cell nucleus, and it also captures the autofluorescence of endogenous fluorophores in its confocal fluorescence microscopy subsystem to offer eosin-like contrast for visualization of the cytoplasm. Parallel acquisition of photoacoustic and autofluorescence images enables comprehensive observations of co-registered cellular structures and organizations, corresponding closely to the typical histological landscapes obtained via standard H&E staining. The HOME demonstrates fast and accurate histopathological imaging of fresh murine tissue and unprocessed clinical glioma specimens and is, therefore, promising for use in assisting intraoperative pathological assessments and performing basic scientific research on neoplasms. |
| format | Article |
| id | doaj-art-638b93801f934f948be8fed8111d3630 |
| institution | Kabale University |
| issn | 2378-0967 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | AIP Publishing LLC |
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| series | APL Photonics |
| spelling | doaj-art-638b93801f934f948be8fed8111d36302024-12-04T17:05:05ZengAIP Publishing LLCAPL Photonics2378-09672024-11-01911116102116102-1410.1063/5.0232343Multiplexing ultraviolet-excited ultrasound and autofluorescence enables slide-free and label-free intraoperative histopathology imagingWei Song0Xiaobao Wang1Yiyan Zhuang2Yechen Wang3Qinlian Ye4Ya-chao Wang5Xiaocong Yuan6Nanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology, Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, ChinaNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology, Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, ChinaNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology, Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, ChinaNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology, Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, ChinaDepart of Neurosurgery, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen 518060, ChinaDepart of Neurosurgery, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen 518060, ChinaNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology, Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, ChinaHistological examination of tissue remains the gold standard for analysis of various diseases in both clinical diagnosis and basic research. However, long-standing challenges in conventional histology have arisen from complex sample preparations involving micrometer-thick sectioning and hematoxylin and eosin (H&E) staining processes, which lead to significant delays in accessing the diagnostic output. We propose a hybrid optical-ultrasonic microscope (HOME) that multiplexes the intrinsic thermoelastic ultrasound and fluorescence emission generated by endogenous biomolecules under single-shot ultraviolet excitation to achieve direct histopathological imaging of fresh tissue without the sectioning and staining steps. The HOME detects ultrasounds of DNA/RNA using a photoacoustic microscopy subsystem to provide a hematoxylin-like contrast that specifically highlights the cell nucleus, and it also captures the autofluorescence of endogenous fluorophores in its confocal fluorescence microscopy subsystem to offer eosin-like contrast for visualization of the cytoplasm. Parallel acquisition of photoacoustic and autofluorescence images enables comprehensive observations of co-registered cellular structures and organizations, corresponding closely to the typical histological landscapes obtained via standard H&E staining. The HOME demonstrates fast and accurate histopathological imaging of fresh murine tissue and unprocessed clinical glioma specimens and is, therefore, promising for use in assisting intraoperative pathological assessments and performing basic scientific research on neoplasms.http://dx.doi.org/10.1063/5.0232343 |
| spellingShingle | Wei Song Xiaobao Wang Yiyan Zhuang Yechen Wang Qinlian Ye Ya-chao Wang Xiaocong Yuan Multiplexing ultraviolet-excited ultrasound and autofluorescence enables slide-free and label-free intraoperative histopathology imaging APL Photonics |
| title | Multiplexing ultraviolet-excited ultrasound and autofluorescence enables slide-free and label-free intraoperative histopathology imaging |
| title_full | Multiplexing ultraviolet-excited ultrasound and autofluorescence enables slide-free and label-free intraoperative histopathology imaging |
| title_fullStr | Multiplexing ultraviolet-excited ultrasound and autofluorescence enables slide-free and label-free intraoperative histopathology imaging |
| title_full_unstemmed | Multiplexing ultraviolet-excited ultrasound and autofluorescence enables slide-free and label-free intraoperative histopathology imaging |
| title_short | Multiplexing ultraviolet-excited ultrasound and autofluorescence enables slide-free and label-free intraoperative histopathology imaging |
| title_sort | multiplexing ultraviolet excited ultrasound and autofluorescence enables slide free and label free intraoperative histopathology imaging |
| url | http://dx.doi.org/10.1063/5.0232343 |
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