Prevalence of subclinical mastitis in cows, isolation of agents and determination of their antibiotic susceptibility
<b>Aim:</b> To investigate bacterial agents causing subclinical mastitis in cow husbandry in Kars province, Turkey.<p> <b>Materials and Methods:</b> Subclinical mastitis was identified in 120 (24%) of 500 dairy cows screened by CMT. Within the scope of the study, it was...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1205 |
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| author | Aliye Gülmez Sağlam Salih Otlu Mustafa Reha Çoşkun Elif Çelik Fatih Büyük Mitat Şahin |
| author_facet | Aliye Gülmez Sağlam Salih Otlu Mustafa Reha Çoşkun Elif Çelik Fatih Büyük Mitat Şahin |
| author_sort | Aliye Gülmez Sağlam |
| collection | DOAJ |
| description | <b>Aim:</b> To investigate bacterial agents causing subclinical mastitis
in cow husbandry in Kars province, Turkey.<p>
<b>Materials and Methods:</b> Subclinical mastitis was identified
in 120 (24%) of 500 dairy cows screened by CMT. Within the
scope of the study, it was taken milk samples from 120 dairy
cows and evaluated in the laboratory. For bacterial isolation, the
milk samples were mixed with vortex and 100 μl of milk were
streaked on Blood Agar (with 7% sheep blood) and Eosin Methylene
Blue Agar. For Mycoplasma spp. isolation, milk samples
were transferred to PPLO broth and PPLO agar. For phenotypic
identification, cultures were evaluated according to the colony
morphology, hemolysis on BA and Gram staining properties,
and then were subjected to biochemical tests. Antibiotic susceptibilities
of isolates were determined by Kirby Bauer Disc
Diffusion Test.<p>
<b>Results:</b> As a result of bacteriological studies, 229 Staphylococcus
spp., 7 Corynebacterium spp., 16 Bacillus spp., 5 Acinetobacter
spp., 2 Escherichia coli, and 2 Mycoplasma spp. were isolated
from milk samples of 120 cows. As a result of antibiogram, the
majority of isolates have found sensitive to cefoxitin (30 μg) and
imipenem (10 μg) and the highest resistance among the isolates
was determined against to penicillin (10 μg).<p>
<b>Conclusion: </b>The screening of subclinical mastitis at certain periods
in enterprises, the proper characterization to be determined
by isolation and identification of agent are crucial for the
profitability and sustainability of dairy farming. |
| format | Article |
| id | doaj-art-6367a9dd373d4b5cbce220b4a9fb8f0c |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-6367a9dd373d4b5cbce220b4a9fb8f0c2024-12-02T09:05:38ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-195334292981205Prevalence of subclinical mastitis in cows, isolation of agents and determination of their antibiotic susceptibilityAliye Gülmez SağlamSalih OtluMustafa Reha ÇoşkunElif ÇelikFatih BüyükMitat Şahin<b>Aim:</b> To investigate bacterial agents causing subclinical mastitis in cow husbandry in Kars province, Turkey.<p> <b>Materials and Methods:</b> Subclinical mastitis was identified in 120 (24%) of 500 dairy cows screened by CMT. Within the scope of the study, it was taken milk samples from 120 dairy cows and evaluated in the laboratory. For bacterial isolation, the milk samples were mixed with vortex and 100 μl of milk were streaked on Blood Agar (with 7% sheep blood) and Eosin Methylene Blue Agar. For Mycoplasma spp. isolation, milk samples were transferred to PPLO broth and PPLO agar. For phenotypic identification, cultures were evaluated according to the colony morphology, hemolysis on BA and Gram staining properties, and then were subjected to biochemical tests. Antibiotic susceptibilities of isolates were determined by Kirby Bauer Disc Diffusion Test.<p> <b>Results:</b> As a result of bacteriological studies, 229 Staphylococcus spp., 7 Corynebacterium spp., 16 Bacillus spp., 5 Acinetobacter spp., 2 Escherichia coli, and 2 Mycoplasma spp. were isolated from milk samples of 120 cows. As a result of antibiogram, the majority of isolates have found sensitive to cefoxitin (30 μg) and imipenem (10 μg) and the highest resistance among the isolates was determined against to penicillin (10 μg).<p> <b>Conclusion: </b>The screening of subclinical mastitis at certain periods in enterprises, the proper characterization to be determined by isolation and identification of agent are crucial for the profitability and sustainability of dairy farming.http://eurasianjvetsci.org/pdf.php3?id=1205subclinical mastitisdairy cowprevalencebacteriaantibiotic susceptibility |
| spellingShingle | Aliye Gülmez Sağlam Salih Otlu Mustafa Reha Çoşkun Elif Çelik Fatih Büyük Mitat Şahin Prevalence of subclinical mastitis in cows, isolation of agents and determination of their antibiotic susceptibility Eurasian Journal of Veterinary Sciences subclinical mastitis dairy cow prevalence bacteria antibiotic susceptibility |
| title | Prevalence of subclinical mastitis in cows, isolation of agents and determination of their antibiotic susceptibility |
| title_full | Prevalence of subclinical mastitis in cows, isolation of agents and determination of their antibiotic susceptibility |
| title_fullStr | Prevalence of subclinical mastitis in cows, isolation of agents and determination of their antibiotic susceptibility |
| title_full_unstemmed | Prevalence of subclinical mastitis in cows, isolation of agents and determination of their antibiotic susceptibility |
| title_short | Prevalence of subclinical mastitis in cows, isolation of agents and determination of their antibiotic susceptibility |
| title_sort | prevalence of subclinical mastitis in cows isolation of agents and determination of their antibiotic susceptibility |
| topic | subclinical mastitis dairy cow prevalence bacteria antibiotic susceptibility |
| url | http://eurasianjvetsci.org/pdf.php3?id=1205 |
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