Prevalence of subclinical mastitis in cows, isolation of agents and determination of their antibiotic susceptibility
<b>Aim:</b> To investigate bacterial agents causing subclinical mastitis in cow husbandry in Kars province, Turkey.<p> <b>Materials and Methods:</b> Subclinical mastitis was identified in 120 (24%) of 500 dairy cows screened by CMT. Within the scope of the study, it was...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1205 |
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| Summary: | <b>Aim:</b> To investigate bacterial agents causing subclinical mastitis
in cow husbandry in Kars province, Turkey.<p>
<b>Materials and Methods:</b> Subclinical mastitis was identified
in 120 (24%) of 500 dairy cows screened by CMT. Within the
scope of the study, it was taken milk samples from 120 dairy
cows and evaluated in the laboratory. For bacterial isolation, the
milk samples were mixed with vortex and 100 μl of milk were
streaked on Blood Agar (with 7% sheep blood) and Eosin Methylene
Blue Agar. For Mycoplasma spp. isolation, milk samples
were transferred to PPLO broth and PPLO agar. For phenotypic
identification, cultures were evaluated according to the colony
morphology, hemolysis on BA and Gram staining properties,
and then were subjected to biochemical tests. Antibiotic susceptibilities
of isolates were determined by Kirby Bauer Disc
Diffusion Test.<p>
<b>Results:</b> As a result of bacteriological studies, 229 Staphylococcus
spp., 7 Corynebacterium spp., 16 Bacillus spp., 5 Acinetobacter
spp., 2 Escherichia coli, and 2 Mycoplasma spp. were isolated
from milk samples of 120 cows. As a result of antibiogram, the
majority of isolates have found sensitive to cefoxitin (30 μg) and
imipenem (10 μg) and the highest resistance among the isolates
was determined against to penicillin (10 μg).<p>
<b>Conclusion: </b>The screening of subclinical mastitis at certain periods
in enterprises, the proper characterization to be determined
by isolation and identification of agent are crucial for the
profitability and sustainability of dairy farming. |
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| ISSN: | 1309-6958 2146-1953 |