Multidrug Resistance of <i>Gallibacterium anatis</i> Biovar Haemolytica Isolated from the Reproductive Tracts of Laying Hens
Antimicrobial resistance is recognized worldwide as one of the greatest threats to human and animal health and the environment. To evaluate the resistance rate of <i>Gallibacterium anatis</i> biovar haemolytica, which contributes to bacteremia, oophoritis, ovarian follicle degeneration,...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/13/11/989 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846152711034109952 |
|---|---|
| author | Olimpia Kursa |
| author_facet | Olimpia Kursa |
| author_sort | Olimpia Kursa |
| collection | DOAJ |
| description | Antimicrobial resistance is recognized worldwide as one of the greatest threats to human and animal health and the environment. To evaluate the resistance rate of <i>Gallibacterium anatis</i> biovar haemolytica, which contributes to bacteremia, oophoritis, ovarian follicle degeneration, salpingitis, decreased egg production, and increased mortality in hens, strains isolated from the reproductive tracts of layers were analyzed. The oviducts were taken from three hens from each of 10 flocks manifesting clinical signs related to laying. Twenty-two isolates of <i>G. anatis</i> biovar haemolytica collected from the three parts of the reproductive system were identified using MALDI-TOF and molecular methods. The biovar’s resistance to 19 antimicrobial substances was assessed using the disk diffusion (n = 8) and broth microdilution (n = 11) methods. The presence of virulence (<i>gtxA</i>, <i>gyrB</i>, and <i>flfA</i>) and antibiotic resistance (<i>bla<sub>ROB</sub></i>, <i>aphA</i>, <i>tetB</i>, and <i>tetH</i>) genes was examined using PCR. All the isolates were resistant to four or more classes of antibiotics and were considered multidrug-resistant. All such isolates were resistant to tilmicosin, tylosin, and enrofloxacin, 88.2% were to tetracycline, and 82.4% to vancomycin. The <i>gtxA</i>, <i>gyrB</i>, <i>tetB</i>, and <i>tetH</i> genes were demonstrated. Considering the present prevalence of multidrug resistance among <i>G. anatis</i> biovar haemolytica isolates from laying hen reproductive tracts, surveillance in reproductive flocks is warranted. |
| format | Article |
| id | doaj-art-635c892dcef34b35b5a00d674a83d2b2 |
| institution | Kabale University |
| issn | 2076-0817 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-635c892dcef34b35b5a00d674a83d2b22024-11-26T18:16:54ZengMDPI AGPathogens2076-08172024-11-01131198910.3390/pathogens13110989Multidrug Resistance of <i>Gallibacterium anatis</i> Biovar Haemolytica Isolated from the Reproductive Tracts of Laying HensOlimpia Kursa0Department of Poultry Diseases, National Veterinary Research Institute, 24-100 Puławy, PolandAntimicrobial resistance is recognized worldwide as one of the greatest threats to human and animal health and the environment. To evaluate the resistance rate of <i>Gallibacterium anatis</i> biovar haemolytica, which contributes to bacteremia, oophoritis, ovarian follicle degeneration, salpingitis, decreased egg production, and increased mortality in hens, strains isolated from the reproductive tracts of layers were analyzed. The oviducts were taken from three hens from each of 10 flocks manifesting clinical signs related to laying. Twenty-two isolates of <i>G. anatis</i> biovar haemolytica collected from the three parts of the reproductive system were identified using MALDI-TOF and molecular methods. The biovar’s resistance to 19 antimicrobial substances was assessed using the disk diffusion (n = 8) and broth microdilution (n = 11) methods. The presence of virulence (<i>gtxA</i>, <i>gyrB</i>, and <i>flfA</i>) and antibiotic resistance (<i>bla<sub>ROB</sub></i>, <i>aphA</i>, <i>tetB</i>, and <i>tetH</i>) genes was examined using PCR. All the isolates were resistant to four or more classes of antibiotics and were considered multidrug-resistant. All such isolates were resistant to tilmicosin, tylosin, and enrofloxacin, 88.2% were to tetracycline, and 82.4% to vancomycin. The <i>gtxA</i>, <i>gyrB</i>, <i>tetB</i>, and <i>tetH</i> genes were demonstrated. Considering the present prevalence of multidrug resistance among <i>G. anatis</i> biovar haemolytica isolates from laying hen reproductive tracts, surveillance in reproductive flocks is warranted.https://www.mdpi.com/2076-0817/13/11/989<i>Gallibacterium anatis</i>biovar haemolyticamultidrug resistanceoviductlaying hens |
| spellingShingle | Olimpia Kursa Multidrug Resistance of <i>Gallibacterium anatis</i> Biovar Haemolytica Isolated from the Reproductive Tracts of Laying Hens Pathogens <i>Gallibacterium anatis</i> biovar haemolytica multidrug resistance oviduct laying hens |
| title | Multidrug Resistance of <i>Gallibacterium anatis</i> Biovar Haemolytica Isolated from the Reproductive Tracts of Laying Hens |
| title_full | Multidrug Resistance of <i>Gallibacterium anatis</i> Biovar Haemolytica Isolated from the Reproductive Tracts of Laying Hens |
| title_fullStr | Multidrug Resistance of <i>Gallibacterium anatis</i> Biovar Haemolytica Isolated from the Reproductive Tracts of Laying Hens |
| title_full_unstemmed | Multidrug Resistance of <i>Gallibacterium anatis</i> Biovar Haemolytica Isolated from the Reproductive Tracts of Laying Hens |
| title_short | Multidrug Resistance of <i>Gallibacterium anatis</i> Biovar Haemolytica Isolated from the Reproductive Tracts of Laying Hens |
| title_sort | multidrug resistance of i gallibacterium anatis i biovar haemolytica isolated from the reproductive tracts of laying hens |
| topic | <i>Gallibacterium anatis</i> biovar haemolytica multidrug resistance oviduct laying hens |
| url | https://www.mdpi.com/2076-0817/13/11/989 |
| work_keys_str_mv | AT olimpiakursa multidrugresistanceofigallibacteriumanatisibiovarhaemolyticaisolatedfromthereproductivetractsoflayinghens |