Evaluation of chitinase production ability to antagonize Pyricularia Oryzae of bacteria and actinomycete strains isolated from rice root soil in Kien Giang Province
The fungus Pyricularia oryzae is one of the agents causing rice blast disease. The mechanism of the infection is directing penetration through the plasma membrane, secreting cytotoxins, and forming infectious cells specialized for causing disease in rice plants in some species (Muni & Nadarajah,...
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| Format: | Article |
| Language: | English |
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HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE
2024-09-01
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| Series: | Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ |
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| Online Access: | https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/3507 |
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| author | Đinh Anh Hòa Trần Thị Phấn Trần Thùy Trang Nguyễn Thị Ánh Nguyệt Nguyễn Thị Thùy Dương Nguyễn Hải An Lê Thị Mai Châm |
| author_facet | Đinh Anh Hòa Trần Thị Phấn Trần Thùy Trang Nguyễn Thị Ánh Nguyệt Nguyễn Thị Thùy Dương Nguyễn Hải An Lê Thị Mai Châm |
| author_sort | Đinh Anh Hòa |
| collection | DOAJ |
| description | The fungus Pyricularia oryzae is one of the agents causing rice blast disease. The mechanism of the infection is directing penetration through the plasma membrane, secreting cytotoxins, and forming infectious cells specialized for causing disease in rice plants in some species (Muni & Nadarajah, 2014). Therefore, biological products containing microorganisms that can antagonize fungal pathogens and are environmentally friendly, are promising measure to control this disease. Among the beneficial microorganisms, bacteria and rhizosphere actinomycetes are known as potential antagonistic groups in disease prevention caused by fungi. This study screened 11 actinomycetes and 10 bacteria with high chitinase activity from 09 rice rhizosphere soil samples in Kien Giang province. Among them, strains B.KG4.1 and S.KG1.1 have strong potential to inhibit Pyricularia oryzae fungus by co-culture method with antagonistic effectiveness reaching 90.9% and 87.41% after 15 days, respectively. Antagonistic activity to fungal pathogens of bacterial strains was positively correlated with their ability to secrete extracellular chitinase. Based on the morphological characteristics and 16S-rRNA gene sequencing, strain S.KG1.1 was identified as Streptomyces carpinensis and strain B.KG4.1 belongs to the Bacillus amyloliquefaciens group. With these characteristics, Bacillus amyloliquefaciens B.KG4.1 and Streptomyces carpinensis S.KG1.1 have the potential to be exploited in the production of biological products to control the fungus Pyricularia oryzae causing rice blast disease. |
| format | Article |
| id | doaj-art-633c1c6d74824d399e81db8b1900c45e |
| institution | Kabale University |
| issn | 2734-9322 2734-9594 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE |
| record_format | Article |
| series | Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ |
| spelling | doaj-art-633c1c6d74824d399e81db8b1900c45e2025-01-11T06:53:00ZengHO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCETạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ2734-93222734-95942024-09-01192506610.46223/HCMCOUJS.tech.vi.19.2.3507.20242216Evaluation of chitinase production ability to antagonize Pyricularia Oryzae of bacteria and actinomycete strains isolated from rice root soil in Kien Giang ProvinceĐinh Anh Hòa0Trần Thị Phấn1Trần Thùy Trang2Nguyễn Thị Ánh Nguyệt3Nguyễn Thị Thùy Dương4Nguyễn Hải An5Lê Thị Mai Châm6Trung tâm Công nghệ Sinh học Thành phố Hồ Chí Minh, Thành phố Hồ Chí MinhTrung tâm Công nghệ Sinh học Thành phố Hồ Chí Minh, Thành phố Hồ Chí MinhTrung tâm Công nghệ Sinh học Thành phố Hồ Chí Minh, Thành phố Hồ Chí MinhTrung tâm Công nghệ Sinh học Thành phố Hồ Chí Minh, Thành phố Hồ Chí MinhTrung tâm Công nghệ Sinh học Thành phố Hồ Chí Minh, Thành phố Hồ Chí MinhTrung tâm Công nghệ Sinh học Thành phố Hồ Chí Minh, Thành phố Hồ Chí MinhTrung tâm Công nghệ Sinh học Thành phố Hồ Chí Minh, Thành phố Hồ Chí MinhThe fungus Pyricularia oryzae is one of the agents causing rice blast disease. The mechanism of the infection is directing penetration through the plasma membrane, secreting cytotoxins, and forming infectious cells specialized for causing disease in rice plants in some species (Muni & Nadarajah, 2014). Therefore, biological products containing microorganisms that can antagonize fungal pathogens and are environmentally friendly, are promising measure to control this disease. Among the beneficial microorganisms, bacteria and rhizosphere actinomycetes are known as potential antagonistic groups in disease prevention caused by fungi. This study screened 11 actinomycetes and 10 bacteria with high chitinase activity from 09 rice rhizosphere soil samples in Kien Giang province. Among them, strains B.KG4.1 and S.KG1.1 have strong potential to inhibit Pyricularia oryzae fungus by co-culture method with antagonistic effectiveness reaching 90.9% and 87.41% after 15 days, respectively. Antagonistic activity to fungal pathogens of bacterial strains was positively correlated with their ability to secrete extracellular chitinase. Based on the morphological characteristics and 16S-rRNA gene sequencing, strain S.KG1.1 was identified as Streptomyces carpinensis and strain B.KG4.1 belongs to the Bacillus amyloliquefaciens group. With these characteristics, Bacillus amyloliquefaciens B.KG4.1 and Streptomyces carpinensis S.KG1.1 have the potential to be exploited in the production of biological products to control the fungus Pyricularia oryzae causing rice blast disease.https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/3507bacillus sp., bệnh đạo ônpyricularia oryzaestreptomyces carpinensisvi sinh vật vùng rễ |
| spellingShingle | Đinh Anh Hòa Trần Thị Phấn Trần Thùy Trang Nguyễn Thị Ánh Nguyệt Nguyễn Thị Thùy Dương Nguyễn Hải An Lê Thị Mai Châm Evaluation of chitinase production ability to antagonize Pyricularia Oryzae of bacteria and actinomycete strains isolated from rice root soil in Kien Giang Province Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ bacillus sp., bệnh đạo ôn pyricularia oryzae streptomyces carpinensis vi sinh vật vùng rễ |
| title | Evaluation of chitinase production ability to antagonize Pyricularia Oryzae of bacteria and actinomycete strains isolated from rice root soil in Kien Giang Province |
| title_full | Evaluation of chitinase production ability to antagonize Pyricularia Oryzae of bacteria and actinomycete strains isolated from rice root soil in Kien Giang Province |
| title_fullStr | Evaluation of chitinase production ability to antagonize Pyricularia Oryzae of bacteria and actinomycete strains isolated from rice root soil in Kien Giang Province |
| title_full_unstemmed | Evaluation of chitinase production ability to antagonize Pyricularia Oryzae of bacteria and actinomycete strains isolated from rice root soil in Kien Giang Province |
| title_short | Evaluation of chitinase production ability to antagonize Pyricularia Oryzae of bacteria and actinomycete strains isolated from rice root soil in Kien Giang Province |
| title_sort | evaluation of chitinase production ability to antagonize pyricularia oryzae of bacteria and actinomycete strains isolated from rice root soil in kien giang province |
| topic | bacillus sp., bệnh đạo ôn pyricularia oryzae streptomyces carpinensis vi sinh vật vùng rễ |
| url | https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/3507 |
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