Gene expression and immunohistochemistry analysis of ADAMTS-1 and its substrates in odontogenic keratocyst
Abstract Background Considering the significant participation of the microenvironment in the local aggressiveness of odontogenic keratocysts, this study aims to evaluate the expression of ADAMTS-1 and its substrates, versican, aggrecan and brevican in this locally invasive odontogenic cyst. Methods...
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BMC
2025-01-01
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| Series: | Diagnostic Pathology |
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| Online Access: | https://doi.org/10.1186/s13000-024-01576-0 |
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| author | Osvaldo Rodrigues de Souza Neto Antonia Taiane Lopes de Moraes Hellen Thais Fuzii Antonio Guilherme Maneschy Faria Vanessa Morais Freitas Maria Sueli da Silva Kataoka Sérgio de Melo Alves João de Jesus Viana Pinheiro |
| author_facet | Osvaldo Rodrigues de Souza Neto Antonia Taiane Lopes de Moraes Hellen Thais Fuzii Antonio Guilherme Maneschy Faria Vanessa Morais Freitas Maria Sueli da Silva Kataoka Sérgio de Melo Alves João de Jesus Viana Pinheiro |
| author_sort | Osvaldo Rodrigues de Souza Neto |
| collection | DOAJ |
| description | Abstract Background Considering the significant participation of the microenvironment in the local aggressiveness of odontogenic keratocysts, this study aims to evaluate the expression of ADAMTS-1 and its substrates, versican, aggrecan and brevican in this locally invasive odontogenic cyst. Methods Immunohistochemistry and polymerase chain reaction (PCR) were conducted on 30 cases of odontogenic keratocysts (OKCs) and 20 dental follicles (DFs). Results The immunohistochemical expression of these proteins was predominantly cytoplasmic and granular across all samples. In epithelial tissue, the immunoexpression of aggrecan and versican was higher in OKC (p < 0.05) compared to DF. Comparing the expression of proteins between the OKC epithelium and the cystic capsule, it was observed that all molecules were more expressed in the epithelium (p < 0.001). RT-PCR confirmed the expression of ADAMTS-1 and proteoglycans in all samples. Conclusion ADAMTS-1, aggrecan, brevican, and versican were expressed in all samples with a granular and cytoplasmic pattern. RT-PCR confirmed their presence in both OKC and DF, but only aggrecan and versican exhibited significantly higher levels in OKC (p < 0.05). Protein expression was notably greater in the epithelial component of OKC. These findings underscore the potential role of these proteins in the biological behavior of OKC. |
| format | Article |
| id | doaj-art-62d32e285649428f8b44461efc1ce172 |
| institution | Kabale University |
| issn | 1746-1596 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Diagnostic Pathology |
| spelling | doaj-art-62d32e285649428f8b44461efc1ce1722025-01-12T12:05:58ZengBMCDiagnostic Pathology1746-15962025-01-0120111110.1186/s13000-024-01576-0Gene expression and immunohistochemistry analysis of ADAMTS-1 and its substrates in odontogenic keratocystOsvaldo Rodrigues de Souza Neto0Antonia Taiane Lopes de Moraes1Hellen Thais Fuzii2Antonio Guilherme Maneschy Faria3Vanessa Morais Freitas4Maria Sueli da Silva Kataoka5Sérgio de Melo Alves6João de Jesus Viana Pinheiro7Cell Culture Laboratory, School of Dentistry, Federal University of ParaOral Diagnosis Department, Piracicaba Dental School, University of Campinas (UNICAMP)Immunopathology Laboratory, Tropical Medicine Institute, Federal University of ParáSchool of Dentistry, Federal University of ParaDepartment of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São PauloCell Culture Laboratory, School of Dentistry, Federal University of ParaCell Culture Laboratory, School of Dentistry, Federal University of ParaCell Culture Laboratory, School of Dentistry, Federal University of ParaAbstract Background Considering the significant participation of the microenvironment in the local aggressiveness of odontogenic keratocysts, this study aims to evaluate the expression of ADAMTS-1 and its substrates, versican, aggrecan and brevican in this locally invasive odontogenic cyst. Methods Immunohistochemistry and polymerase chain reaction (PCR) were conducted on 30 cases of odontogenic keratocysts (OKCs) and 20 dental follicles (DFs). Results The immunohistochemical expression of these proteins was predominantly cytoplasmic and granular across all samples. In epithelial tissue, the immunoexpression of aggrecan and versican was higher in OKC (p < 0.05) compared to DF. Comparing the expression of proteins between the OKC epithelium and the cystic capsule, it was observed that all molecules were more expressed in the epithelium (p < 0.001). RT-PCR confirmed the expression of ADAMTS-1 and proteoglycans in all samples. Conclusion ADAMTS-1, aggrecan, brevican, and versican were expressed in all samples with a granular and cytoplasmic pattern. RT-PCR confirmed their presence in both OKC and DF, but only aggrecan and versican exhibited significantly higher levels in OKC (p < 0.05). Protein expression was notably greater in the epithelial component of OKC. These findings underscore the potential role of these proteins in the biological behavior of OKC.https://doi.org/10.1186/s13000-024-01576-0Odontogenic keratocystADAMTS-1ProteoglycansImmunohistochemistry |
| spellingShingle | Osvaldo Rodrigues de Souza Neto Antonia Taiane Lopes de Moraes Hellen Thais Fuzii Antonio Guilherme Maneschy Faria Vanessa Morais Freitas Maria Sueli da Silva Kataoka Sérgio de Melo Alves João de Jesus Viana Pinheiro Gene expression and immunohistochemistry analysis of ADAMTS-1 and its substrates in odontogenic keratocyst Diagnostic Pathology Odontogenic keratocyst ADAMTS-1 Proteoglycans Immunohistochemistry |
| title | Gene expression and immunohistochemistry analysis of ADAMTS-1 and its substrates in odontogenic keratocyst |
| title_full | Gene expression and immunohistochemistry analysis of ADAMTS-1 and its substrates in odontogenic keratocyst |
| title_fullStr | Gene expression and immunohistochemistry analysis of ADAMTS-1 and its substrates in odontogenic keratocyst |
| title_full_unstemmed | Gene expression and immunohistochemistry analysis of ADAMTS-1 and its substrates in odontogenic keratocyst |
| title_short | Gene expression and immunohistochemistry analysis of ADAMTS-1 and its substrates in odontogenic keratocyst |
| title_sort | gene expression and immunohistochemistry analysis of adamts 1 and its substrates in odontogenic keratocyst |
| topic | Odontogenic keratocyst ADAMTS-1 Proteoglycans Immunohistochemistry |
| url | https://doi.org/10.1186/s13000-024-01576-0 |
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