Protocol for detection of tRNA-derived fragments in cells, tissues, and plasma
Summary: tRNA-derived fragments (tRFs) are frequently dysregulated in cancers, and approaches for the detection of tRFs within biological samples are vital for their expression analysis and functional exploration. Here, we present a protocol for detecting tRFs using a modified TaqMan quantitative re...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724006002 |
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| author | Mengqian Yu Pengyuan Liu Yan Lu |
| author_facet | Mengqian Yu Pengyuan Liu Yan Lu |
| author_sort | Mengqian Yu |
| collection | DOAJ |
| description | Summary: tRNA-derived fragments (tRFs) are frequently dysregulated in cancers, and approaches for the detection of tRFs within biological samples are vital for their expression analysis and functional exploration. Here, we present a protocol for detecting tRFs using a modified TaqMan quantitative real-time PCR (qRT-PCR)-based technique, Dumbbell-PCR (Db-PCR). We describe steps for primer and adapter design, adapter-RNA ligation, and RNA detection. This protocol streamlines and enhances the precision of tRF quantification in cells, tissues, and plasma, facilitating a time-efficient and reliable assessment of their presence.For complete details on the use and execution of this protocol, please refer to Yu et al.1 and Sun et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-6214b8314b3c40a59cf5fcc376527de0 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-6214b8314b3c40a59cf5fcc376527de02024-11-20T05:08:12ZengElsevierSTAR Protocols2666-16672024-12-0154103435Protocol for detection of tRNA-derived fragments in cells, tissues, and plasmaMengqian Yu0Pengyuan Liu1Yan Lu2Zhejiang Provincial Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases, Women’s Hospital and Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, China; Corresponding authorDepartment of Respiratory Medicine, Sir Run Run Shaw Hospital and Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310016, ChinaZhejiang Provincial Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases, Women’s Hospital and Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, China; Corresponding authorSummary: tRNA-derived fragments (tRFs) are frequently dysregulated in cancers, and approaches for the detection of tRFs within biological samples are vital for their expression analysis and functional exploration. Here, we present a protocol for detecting tRFs using a modified TaqMan quantitative real-time PCR (qRT-PCR)-based technique, Dumbbell-PCR (Db-PCR). We describe steps for primer and adapter design, adapter-RNA ligation, and RNA detection. This protocol streamlines and enhances the precision of tRF quantification in cells, tissues, and plasma, facilitating a time-efficient and reliable assessment of their presence.For complete details on the use and execution of this protocol, please refer to Yu et al.1 and Sun et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724006002CancerMolecular BiologyGene Expression |
| spellingShingle | Mengqian Yu Pengyuan Liu Yan Lu Protocol for detection of tRNA-derived fragments in cells, tissues, and plasma STAR Protocols Cancer Molecular Biology Gene Expression |
| title | Protocol for detection of tRNA-derived fragments in cells, tissues, and plasma |
| title_full | Protocol for detection of tRNA-derived fragments in cells, tissues, and plasma |
| title_fullStr | Protocol for detection of tRNA-derived fragments in cells, tissues, and plasma |
| title_full_unstemmed | Protocol for detection of tRNA-derived fragments in cells, tissues, and plasma |
| title_short | Protocol for detection of tRNA-derived fragments in cells, tissues, and plasma |
| title_sort | protocol for detection of trna derived fragments in cells tissues and plasma |
| topic | Cancer Molecular Biology Gene Expression |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724006002 |
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