Circular RNA NINL accelerates the malignant progression of cervical cancer

Abstract Objective The study’s aim was to explore a novel miRNA/mRNA network mediated by circNINL in cervical cancer (CC). Methods Tumor tissue specimens and normal tissue specimens of 86 CC patients were collected. CircNINL, miR-2467-3p, and specificity protein 1 (SP1) expression levels in tissues...

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Main Authors: ChengCheng Cao, HaiFeng Zhang, Ting Zhang, CuiCui Nie
Format: Article
Language:English
Published: Springer 2024-12-01
Series:Discover Oncology
Subjects:
Online Access:https://doi.org/10.1007/s12672-024-01588-8
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author ChengCheng Cao
HaiFeng Zhang
Ting Zhang
CuiCui Nie
author_facet ChengCheng Cao
HaiFeng Zhang
Ting Zhang
CuiCui Nie
author_sort ChengCheng Cao
collection DOAJ
description Abstract Objective The study’s aim was to explore a novel miRNA/mRNA network mediated by circNINL in cervical cancer (CC). Methods Tumor tissue specimens and normal tissue specimens of 86 CC patients were collected. CircNINL, miR-2467-3p, and specificity protein 1 (SP1) expression levels in tissues were detected. In Hela cells, transfection was implemented to determine whether CircNINL, miR-2467-3p and SP1 regulated cellular progression. The interaction between circNINL, miR-2467-3p, and SP1 was explored. Results CircNINL and SP1 were abnormally increased whereas miR-2467-3p expression was suppressed in CC. Functionally, circNINL silencing or miR-2467-3p overexpression reduced CC cell progression, whereas circNINL overexpression did the opposite. CircNINL had a spongy effect on miR-2467-3p to regulate SP1 levels. miR-2467-3p inhibition or SP1 overexpression offset the inhibitory effect of circNINL silence on CC malignant progression. Conclusion CircNINL silencing can increase miR-2467-3p, thereby inducing the downregulation of SP1, thereby suppressing CC progression.
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institution Kabale University
issn 2730-6011
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publishDate 2024-12-01
publisher Springer
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series Discover Oncology
spelling doaj-art-61e4a8754f144a7fa863442aa97cdc412024-12-22T12:35:42ZengSpringerDiscover Oncology2730-60112024-12-0115111310.1007/s12672-024-01588-8Circular RNA NINL accelerates the malignant progression of cervical cancerChengCheng Cao0HaiFeng Zhang1Ting Zhang2CuiCui Nie3Department of Gynecology, Affiliated Hospital of Shandong Second Medical UniversityDepartment of Gynecology, Affiliated Hospital of Shandong Second Medical UniversityDepartment of Gynecology and Obstetrics, Sunshine Union HospitalDepartment of Gynecology and Obstetrics, Sunshine Union HospitalAbstract Objective The study’s aim was to explore a novel miRNA/mRNA network mediated by circNINL in cervical cancer (CC). Methods Tumor tissue specimens and normal tissue specimens of 86 CC patients were collected. CircNINL, miR-2467-3p, and specificity protein 1 (SP1) expression levels in tissues were detected. In Hela cells, transfection was implemented to determine whether CircNINL, miR-2467-3p and SP1 regulated cellular progression. The interaction between circNINL, miR-2467-3p, and SP1 was explored. Results CircNINL and SP1 were abnormally increased whereas miR-2467-3p expression was suppressed in CC. Functionally, circNINL silencing or miR-2467-3p overexpression reduced CC cell progression, whereas circNINL overexpression did the opposite. CircNINL had a spongy effect on miR-2467-3p to regulate SP1 levels. miR-2467-3p inhibition or SP1 overexpression offset the inhibitory effect of circNINL silence on CC malignant progression. Conclusion CircNINL silencing can increase miR-2467-3p, thereby inducing the downregulation of SP1, thereby suppressing CC progression.https://doi.org/10.1007/s12672-024-01588-8Cervical cancerCircNINLmiR-2467-3pSpecificity protein 1
spellingShingle ChengCheng Cao
HaiFeng Zhang
Ting Zhang
CuiCui Nie
Circular RNA NINL accelerates the malignant progression of cervical cancer
Discover Oncology
Cervical cancer
CircNINL
miR-2467-3p
Specificity protein 1
title Circular RNA NINL accelerates the malignant progression of cervical cancer
title_full Circular RNA NINL accelerates the malignant progression of cervical cancer
title_fullStr Circular RNA NINL accelerates the malignant progression of cervical cancer
title_full_unstemmed Circular RNA NINL accelerates the malignant progression of cervical cancer
title_short Circular RNA NINL accelerates the malignant progression of cervical cancer
title_sort circular rna ninl accelerates the malignant progression of cervical cancer
topic Cervical cancer
CircNINL
miR-2467-3p
Specificity protein 1
url https://doi.org/10.1007/s12672-024-01588-8
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AT haifengzhang circularrnaninlacceleratesthemalignantprogressionofcervicalcancer
AT tingzhang circularrnaninlacceleratesthemalignantprogressionofcervicalcancer
AT cuicuinie circularrnaninlacceleratesthemalignantprogressionofcervicalcancer