Transcriptome profiling of foxtail millet (Setaria italica) pollen and anther
Abstract Pollen development and germination play a crucial role in the sexual reproduction of plants. This study analysis of transcriptional dynamics of foxtail millet pollen with other tissues and organs (ovule, glume, seedling and root) through RNA-sequencing revealed that a total of 940 genes wer...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-12-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-024-05976-8 |
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| Summary: | Abstract Pollen development and germination play a crucial role in the sexual reproduction of plants. This study analysis of transcriptional dynamics of foxtail millet pollen with other tissues and organs (ovule, glume, seedling and root) through RNA-sequencing revealed that a total of 940 genes were up-regulated in foxtail millet pollen. Based on this, we analyzed the genes involved in pollen tube growth of receptor kinases and small peptides, calcium signaling, small G proteins, vesicle transport, cytoskeleton, cell wall correlation, and transcription factors that are up-regulated in pollen. At the same time, we compared the gene expression of foxtail millet pollen and mature anthers, and found that a large number of transcription factors were specific expressed in mature anthers. In addition, we verified the accuracy of the transcriptome data using RT-qPCR. Finally, employed the antisense Oligonucleotide (as-ODN) system found that inhibiting SiPME67 expression would cause abnormal growth of pollen tube subapical. In summary, we preliminarily analyzed the genes that were up-regulated in foxtail millet pollen, which provided a reference for understanding the male sterility mechanism of foxtail millet in the future and theoretical basis for creating new male sterility lines. |
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| ISSN: | 1471-2229 |