A cost-effective protocol for single-cell RNA sequencing of human skin

A cost-effective protocol for single-cell RNA sequencing of human skin

IntroductionSingle-cell RNA sequencing (scRNAseq) and flow cytometry studies in skin are methodologically complex and costly, limiting their accessibility to researchers worldwide. Ideally, RNA and protein-based analyses should be performed on the same lesion to obtain more comprehensive data. Howev...

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Main Authors: Saba Khoshbakht, Özgür Albayrak, Ergün Tiryaki, Orhan Ağcaoğlu, Ayşe Öktem, Gizem Pınar Sun, Elif Er Gülbezer, Sümeyre Seda Ertekin, Ayşe Boyvat, Atay Vural, Seçil Vural
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-10-01
Series:Frontiers in Immunology
Subjects:
skin
inflammation
single cell RNA sequencing (scRNA)
flow cytometry
souporcell
multiplexing
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2024.1393017/full
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