OsCSLD4 confers salt–alkali tolerance by regulating gene expressions in photosynthesis and carbohydrate biosynthesis pathways, cell wall hemicellulose accumulation and physio-biochemical adaptability in rice
In rice, cell-wall matrix polysaccharides contribute to salt–alkali tolerance. However, the mechanism by which cell-wall matrix polysaccharides and their biosynthetic genes regulate salt–alkali tolerance in rice remains unclear. To address this question, we report on the regulatory mechanism of salt...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Plant Stress |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X24002574 |
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| author | Zhijian Liu Cuili Wu Wenjie Li Li Hu Xingyue Fu Binhua Hu Yongxiang Liao Zufen Xiang Haibo Jiang Weizao Huang Xiaocheng Yang Anping Du Zhigang Pu Shengbin Tu Xinrong Ma Hui Li |
| author_facet | Zhijian Liu Cuili Wu Wenjie Li Li Hu Xingyue Fu Binhua Hu Yongxiang Liao Zufen Xiang Haibo Jiang Weizao Huang Xiaocheng Yang Anping Du Zhigang Pu Shengbin Tu Xinrong Ma Hui Li |
| author_sort | Zhijian Liu |
| collection | DOAJ |
| description | In rice, cell-wall matrix polysaccharides contribute to salt–alkali tolerance. However, the mechanism by which cell-wall matrix polysaccharides and their biosynthetic genes regulate salt–alkali tolerance in rice remains unclear. To address this question, we report on the regulatory mechanism of salt–alkali tolerance of the 1,4-β-d-xylan synthase gene OsCSLD4 in the hemicellulose biosynthesis pathway. Mutant of OsCSLD4, nd1 and its wild-type were analyzed using comprehensive techniques and methods, including phenotyping, gene expression, comparative transcriptomic analysis, qPCR validation, and determination of physio-biochemical indices. We found that the salt–alkali tolerance of nd1 was lower than that of the wild type, and the expression of the OsCSLD4 gene was induced under salt–alkali stress. Comparative transcriptomic analysis revealed that the expression levels of genes involved in photosynthesis, carbohydrate, and cell wall matrix polysaccharide biosynthesis pathways in nd1 seedlings were downregulated compared to those in the wild type under salt–alkali stress. Accordingly, physio-biochemical analysis demonstrated that nd1 seedlings had reduced levels of chlorophyll, total soluble sugar, starch, and hemicellulose, coupled with a significant increase in malondialdehyde content under salt–alkali stress. In essence, the OsCSLD4 gene confers salt–alkali tolerance to rice by regulating the hemicellulose content to strengthen cell wall integrity and enhance intracellular physio-biochemical salt–alkali tolerance at the cellular level, thereby maintaining photosynthetic capacity and growth at the plant level. This study revealed that OsCSLD4 has potential value in molecular breeding for the development of salt-alkali-tolerant rice varieties. |
| format | Article |
| id | doaj-art-61a036e6d1b8491a9d52a5d68cff0af6 |
| institution | Kabale University |
| issn | 2667-064X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj-art-61a036e6d1b8491a9d52a5d68cff0af62024-12-19T11:01:55ZengElsevierPlant Stress2667-064X2024-12-0114100604OsCSLD4 confers salt–alkali tolerance by regulating gene expressions in photosynthesis and carbohydrate biosynthesis pathways, cell wall hemicellulose accumulation and physio-biochemical adaptability in riceZhijian Liu0Cuili Wu1Wenjie Li2Li Hu3Xingyue Fu4Binhua Hu5Yongxiang Liao6Zufen Xiang7Haibo Jiang8Weizao Huang9Xiaocheng Yang10Anping Du11Zhigang Pu12Shengbin Tu13Xinrong Ma14Hui Li15Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610213, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610213, China; College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610213, China; Southwest Jiaotong University, Chengdu 610031, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610213, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610213, ChinaBiotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaCrop Characteristic Resources Creation and Utilization Key Laboratory of Sichuan Province, Mianyang 621023, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610213, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610213, ChinaCollege of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610213, ChinaBiotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610213, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610213, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610213, China; Corresponding author.In rice, cell-wall matrix polysaccharides contribute to salt–alkali tolerance. However, the mechanism by which cell-wall matrix polysaccharides and their biosynthetic genes regulate salt–alkali tolerance in rice remains unclear. To address this question, we report on the regulatory mechanism of salt–alkali tolerance of the 1,4-β-d-xylan synthase gene OsCSLD4 in the hemicellulose biosynthesis pathway. Mutant of OsCSLD4, nd1 and its wild-type were analyzed using comprehensive techniques and methods, including phenotyping, gene expression, comparative transcriptomic analysis, qPCR validation, and determination of physio-biochemical indices. We found that the salt–alkali tolerance of nd1 was lower than that of the wild type, and the expression of the OsCSLD4 gene was induced under salt–alkali stress. Comparative transcriptomic analysis revealed that the expression levels of genes involved in photosynthesis, carbohydrate, and cell wall matrix polysaccharide biosynthesis pathways in nd1 seedlings were downregulated compared to those in the wild type under salt–alkali stress. Accordingly, physio-biochemical analysis demonstrated that nd1 seedlings had reduced levels of chlorophyll, total soluble sugar, starch, and hemicellulose, coupled with a significant increase in malondialdehyde content under salt–alkali stress. In essence, the OsCSLD4 gene confers salt–alkali tolerance to rice by regulating the hemicellulose content to strengthen cell wall integrity and enhance intracellular physio-biochemical salt–alkali tolerance at the cellular level, thereby maintaining photosynthetic capacity and growth at the plant level. This study revealed that OsCSLD4 has potential value in molecular breeding for the development of salt-alkali-tolerant rice varieties.http://www.sciencedirect.com/science/article/pii/S2667064X24002574OsCSLD4Salt–alkali tolerancePhotosynthesisCarbohydrate biosynthesisCell wallHemicellulose |
| spellingShingle | Zhijian Liu Cuili Wu Wenjie Li Li Hu Xingyue Fu Binhua Hu Yongxiang Liao Zufen Xiang Haibo Jiang Weizao Huang Xiaocheng Yang Anping Du Zhigang Pu Shengbin Tu Xinrong Ma Hui Li OsCSLD4 confers salt–alkali tolerance by regulating gene expressions in photosynthesis and carbohydrate biosynthesis pathways, cell wall hemicellulose accumulation and physio-biochemical adaptability in rice Plant Stress OsCSLD4 Salt–alkali tolerance Photosynthesis Carbohydrate biosynthesis Cell wall Hemicellulose |
| title | OsCSLD4 confers salt–alkali tolerance by regulating gene expressions in photosynthesis and carbohydrate biosynthesis pathways, cell wall hemicellulose accumulation and physio-biochemical adaptability in rice |
| title_full | OsCSLD4 confers salt–alkali tolerance by regulating gene expressions in photosynthesis and carbohydrate biosynthesis pathways, cell wall hemicellulose accumulation and physio-biochemical adaptability in rice |
| title_fullStr | OsCSLD4 confers salt–alkali tolerance by regulating gene expressions in photosynthesis and carbohydrate biosynthesis pathways, cell wall hemicellulose accumulation and physio-biochemical adaptability in rice |
| title_full_unstemmed | OsCSLD4 confers salt–alkali tolerance by regulating gene expressions in photosynthesis and carbohydrate biosynthesis pathways, cell wall hemicellulose accumulation and physio-biochemical adaptability in rice |
| title_short | OsCSLD4 confers salt–alkali tolerance by regulating gene expressions in photosynthesis and carbohydrate biosynthesis pathways, cell wall hemicellulose accumulation and physio-biochemical adaptability in rice |
| title_sort | oscsld4 confers salt alkali tolerance by regulating gene expressions in photosynthesis and carbohydrate biosynthesis pathways cell wall hemicellulose accumulation and physio biochemical adaptability in rice |
| topic | OsCSLD4 Salt–alkali tolerance Photosynthesis Carbohydrate biosynthesis Cell wall Hemicellulose |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X24002574 |
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