c-Jun protein effect on regulating CD4<sup>+</sup> and CD8<sup>+</sup> T cell proliferation in H1N1 influenza A virus infected mice
A H1N1 influenza A virus infected mice model was built to evaluate the regulation mechanism of c-Jun protein in T lymphocytes. To determine the DNAzyme Dz13 efficiency in virus-infected animals, a Western-blotting assay was employed to detected total c-Jun (t-c-Jun) and phosphorylation c-Jun (p-c-Ju...
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| Language: | English |
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Zhejiang University Press
2018-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2017.12.132 |
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| _version_ | 1849247475305545728 |
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| author | ZHANG Shouping SHEN Wentao WANG Lirong ZHANG Baizhong |
| author_facet | ZHANG Shouping SHEN Wentao WANG Lirong ZHANG Baizhong |
| author_sort | ZHANG Shouping |
| collection | DOAJ |
| description | A H1N1 influenza A virus infected mice model was built to evaluate the regulation mechanism of c-Jun protein in T lymphocytes. To determine the DNAzyme Dz13 efficiency in virus-infected animals, a Western-blotting assay was employed to detected total c-Jun (t-c-Jun) and phosphorylation c-Jun (p-c-Jun) levels by specific antibody. It was demonstrated that the drug-treated mice markedly suppressed the expression of t-c-Jun and p-c-Jun. Through flow cytometry and quantitative real-time polymerase chain reaction (qRT-PCR), we could find an obvious CD4<sup>+</sup> (helper T lymphocytes) and CD8<sup>+</sup> T (cytotoxic T lymphocyte) cell proliferation and relative cytokine (IFN-γ, IL-6 and IL-10) expression on day 3 and 6 in the virus-infected mice. The Dz13-treated mice with a c-Jun suppression displayed a down-regulation of CD4<sup>+</sup> and CD8<sup>+</sup> T cells in peripheral blood. At the same time, Dz13-treated group demonstrated a significant reduction of inflammatory cytokine (IFN-γ, IL-6 and IL-10) expression. In conclusion, c-Jun protein regulates CD4<sup>+</sup> and CD8<sup>+</sup> T cell proliferation and inflammatory cytokine expression in H1N1 influenza A virus infected mice. |
| format | Article |
| id | doaj-art-6116a84cf3b04459a19e5d5ddc7c0b72 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2018-11-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-6116a84cf3b04459a19e5d5ddc7c0b722025-08-20T03:58:11ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552018-11-014472272610.3785/j.issn.1008-9209.2017.12.13210089209c-Jun protein effect on regulating CD4<sup>+</sup> and CD8<sup>+</sup> T cell proliferation in H1N1 influenza A virus infected miceZHANG ShoupingSHEN WentaoWANG LirongZHANG BaizhongA H1N1 influenza A virus infected mice model was built to evaluate the regulation mechanism of c-Jun protein in T lymphocytes. To determine the DNAzyme Dz13 efficiency in virus-infected animals, a Western-blotting assay was employed to detected total c-Jun (t-c-Jun) and phosphorylation c-Jun (p-c-Jun) levels by specific antibody. It was demonstrated that the drug-treated mice markedly suppressed the expression of t-c-Jun and p-c-Jun. Through flow cytometry and quantitative real-time polymerase chain reaction (qRT-PCR), we could find an obvious CD4<sup>+</sup> (helper T lymphocytes) and CD8<sup>+</sup> T (cytotoxic T lymphocyte) cell proliferation and relative cytokine (IFN-γ, IL-6 and IL-10) expression on day 3 and 6 in the virus-infected mice. The Dz13-treated mice with a c-Jun suppression displayed a down-regulation of CD4<sup>+</sup> and CD8<sup>+</sup> T cells in peripheral blood. At the same time, Dz13-treated group demonstrated a significant reduction of inflammatory cytokine (IFN-γ, IL-6 and IL-10) expression. In conclusion, c-Jun protein regulates CD4<sup>+</sup> and CD8<sup>+</sup> T cell proliferation and inflammatory cytokine expression in H1N1 influenza A virus infected mice.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2017.12.132c-Jun proteininfluenza A virusT lymphocytecytokine |
| spellingShingle | ZHANG Shouping SHEN Wentao WANG Lirong ZHANG Baizhong c-Jun protein effect on regulating CD4<sup>+</sup> and CD8<sup>+</sup> T cell proliferation in H1N1 influenza A virus infected mice 浙江大学学报. 农业与生命科学版 c-Jun protein influenza A virus T lymphocyte cytokine |
| title | c-Jun protein effect on regulating CD4<sup>+</sup> and CD8<sup>+</sup> T cell proliferation in H1N1 influenza A virus infected mice |
| title_full | c-Jun protein effect on regulating CD4<sup>+</sup> and CD8<sup>+</sup> T cell proliferation in H1N1 influenza A virus infected mice |
| title_fullStr | c-Jun protein effect on regulating CD4<sup>+</sup> and CD8<sup>+</sup> T cell proliferation in H1N1 influenza A virus infected mice |
| title_full_unstemmed | c-Jun protein effect on regulating CD4<sup>+</sup> and CD8<sup>+</sup> T cell proliferation in H1N1 influenza A virus infected mice |
| title_short | c-Jun protein effect on regulating CD4<sup>+</sup> and CD8<sup>+</sup> T cell proliferation in H1N1 influenza A virus infected mice |
| title_sort | c jun protein effect on regulating cd4 sup sup and cd8 sup sup t cell proliferation in h1n1 influenza a virus infected mice |
| topic | c-Jun protein influenza A virus T lymphocyte cytokine |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2017.12.132 |
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