CEP signaling coordinates plant immunity with nitrogen status

Abstract Plant endogenous signaling peptides shape growth, development and adaptations to biotic and abiotic stress. Here, we identify C-TERMINALLY ENCODED PEPTIDEs (CEPs) as immune-modulatory phytocytokines in Arabidopsis thaliana. Our data reveals that CEPs induce immune outputs and are required t...

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Main Authors: Jakub Rzemieniewski, Henriette Leicher, Hyun Kyung Lee, Caroline Broyart, Shahran Nayem, Christian Wiese, Julian Maroschek, Zeynep Camgöz, Vilde Olsson Lalun, Michael Anthony Djordjevic, A. Corina Vlot, Ralph Hückelhoven, Julia Santiago, Martin Stegmann
Format: Article
Language:English
Published: Nature Portfolio 2024-12-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-55194-x
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author Jakub Rzemieniewski
Henriette Leicher
Hyun Kyung Lee
Caroline Broyart
Shahran Nayem
Christian Wiese
Julian Maroschek
Zeynep Camgöz
Vilde Olsson Lalun
Michael Anthony Djordjevic
A. Corina Vlot
Ralph Hückelhoven
Julia Santiago
Martin Stegmann
author_facet Jakub Rzemieniewski
Henriette Leicher
Hyun Kyung Lee
Caroline Broyart
Shahran Nayem
Christian Wiese
Julian Maroschek
Zeynep Camgöz
Vilde Olsson Lalun
Michael Anthony Djordjevic
A. Corina Vlot
Ralph Hückelhoven
Julia Santiago
Martin Stegmann
author_sort Jakub Rzemieniewski
collection DOAJ
description Abstract Plant endogenous signaling peptides shape growth, development and adaptations to biotic and abiotic stress. Here, we identify C-TERMINALLY ENCODED PEPTIDEs (CEPs) as immune-modulatory phytocytokines in Arabidopsis thaliana. Our data reveals that CEPs induce immune outputs and are required to mount resistance against the leaf-infecting bacterial pathogen Pseudomonas syringae pv. tomato. We show that effective immunity requires CEP perception by tissue-specific CEP RECEPTOR 1 (CEPR1) and CEPR2. Moreover, we identify the related RECEPTOR-LIKE KINASE 7 (RLK7) as a CEP4-specific CEP receptor contributing to CEP-mediated immunity, suggesting a complex interplay of multiple CEP ligands and receptors in different tissues during biotic stress. CEPs have a known role in the regulation of root growth and systemic nitrogen (N)-demand signaling. We provide evidence that CEPs and their receptors promote immunity in an N status-dependent manner, suggesting a previously unknown molecular crosstalk between plant nutrition and cell surface immunity. We propose that CEPs and their receptors are central regulators for the adaptation of biotic stress responses to plant-available resources.
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spelling doaj-art-606a3281be4b4e3291b6adaae3047ca02024-12-22T12:36:26ZengNature PortfolioNature Communications2041-17232024-12-0115111610.1038/s41467-024-55194-xCEP signaling coordinates plant immunity with nitrogen statusJakub Rzemieniewski0Henriette Leicher1Hyun Kyung Lee2Caroline Broyart3Shahran Nayem4Christian Wiese5Julian Maroschek6Zeynep Camgöz7Vilde Olsson Lalun8Michael Anthony Djordjevic9A. Corina Vlot10Ralph Hückelhoven11Julia Santiago12Martin Stegmann13Phytopathology, TUM School of Life Sciences, Technical University of MunichPhytopathology, TUM School of Life Sciences, Technical University of MunichThe Plant Signaling Mechanisms Laboratory, Department of Plant Molecular Biology, University of LausanneThe Plant Signaling Mechanisms Laboratory, Department of Plant Molecular Biology, University of LausanneHelmholtz Zentrum Munich, Institute of Biochemical Plant PathologyPhytopathology, TUM School of Life Sciences, Technical University of MunichPhytopathology, TUM School of Life Sciences, Technical University of MunichPhytopathology, TUM School of Life Sciences, Technical University of MunichDepartment of Biosciences Section for Genetics and Evolutionary Biology, Department of Biosciences, University of OsloDivision of Plant Science, Australian National UniversityHelmholtz Zentrum Munich, Institute of Biochemical Plant PathologyPhytopathology, TUM School of Life Sciences, Technical University of MunichThe Plant Signaling Mechanisms Laboratory, Department of Plant Molecular Biology, University of LausannePhytopathology, TUM School of Life Sciences, Technical University of MunichAbstract Plant endogenous signaling peptides shape growth, development and adaptations to biotic and abiotic stress. Here, we identify C-TERMINALLY ENCODED PEPTIDEs (CEPs) as immune-modulatory phytocytokines in Arabidopsis thaliana. Our data reveals that CEPs induce immune outputs and are required to mount resistance against the leaf-infecting bacterial pathogen Pseudomonas syringae pv. tomato. We show that effective immunity requires CEP perception by tissue-specific CEP RECEPTOR 1 (CEPR1) and CEPR2. Moreover, we identify the related RECEPTOR-LIKE KINASE 7 (RLK7) as a CEP4-specific CEP receptor contributing to CEP-mediated immunity, suggesting a complex interplay of multiple CEP ligands and receptors in different tissues during biotic stress. CEPs have a known role in the regulation of root growth and systemic nitrogen (N)-demand signaling. We provide evidence that CEPs and their receptors promote immunity in an N status-dependent manner, suggesting a previously unknown molecular crosstalk between plant nutrition and cell surface immunity. We propose that CEPs and their receptors are central regulators for the adaptation of biotic stress responses to plant-available resources.https://doi.org/10.1038/s41467-024-55194-x
spellingShingle Jakub Rzemieniewski
Henriette Leicher
Hyun Kyung Lee
Caroline Broyart
Shahran Nayem
Christian Wiese
Julian Maroschek
Zeynep Camgöz
Vilde Olsson Lalun
Michael Anthony Djordjevic
A. Corina Vlot
Ralph Hückelhoven
Julia Santiago
Martin Stegmann
CEP signaling coordinates plant immunity with nitrogen status
Nature Communications
title CEP signaling coordinates plant immunity with nitrogen status
title_full CEP signaling coordinates plant immunity with nitrogen status
title_fullStr CEP signaling coordinates plant immunity with nitrogen status
title_full_unstemmed CEP signaling coordinates plant immunity with nitrogen status
title_short CEP signaling coordinates plant immunity with nitrogen status
title_sort cep signaling coordinates plant immunity with nitrogen status
url https://doi.org/10.1038/s41467-024-55194-x
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