Accumulation of yam viruses in different parts of white yam and the practice of positive selection technique in seed yam production
The success of Positive Selection Technique (PST) depends on the visual selection of virus-free plants, which may not always be possible due to virus accumulation in plant parts apart from the leaves. This study determined virus accumulations in different plant parts of white yam when practicing PST...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154324003570 |
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| author | Atta Kwesi Aidoo Richard Tuyee Awuah Joseph Nee Lamptey Marian Dorcas Quain Allen Oppong Ruth Naa Ashiokai Prempeh Jakpasu Victor Kofi Afun Esther Agyemang Marfo Linda Appianimaa Abrokwah |
| author_facet | Atta Kwesi Aidoo Richard Tuyee Awuah Joseph Nee Lamptey Marian Dorcas Quain Allen Oppong Ruth Naa Ashiokai Prempeh Jakpasu Victor Kofi Afun Esther Agyemang Marfo Linda Appianimaa Abrokwah |
| author_sort | Atta Kwesi Aidoo |
| collection | DOAJ |
| description | The success of Positive Selection Technique (PST) depends on the visual selection of virus-free plants, which may not always be possible due to virus accumulation in plant parts apart from the leaves. This study determined virus accumulations in different plant parts of white yam when practicing PST. Experimental plots were established at Ejura and Fumesua, and planted with planting materials (sourced from PST-practicing farmers) of three white yam varieties viz. Kpamyo, Dente and Pona. Symptomatic and asymptomatic plants from these fields were selected and yam mosaic virus (YMV), cucumber mosaic virus (CMV) and yam mild mosaic virus (YMMV) detected from their leaves, vines and tubers using DAS-ELISA. Virus concentration in the various plant organs were obtained from the absorbance values using BIO-RAD microplate reader. Disease incidence and severity were assessed at three months after planting. Data were subjected to ANOVA and means separated with LSD using the R software. In the subsequent season, harvested seed yams from symptomatic and asymptomatic plants were studied in the screenhouse and viruses detected in leaves and vines using RT-PCR. Plant viruses were found in all plant parts of the three white yam varieties. Concentration of all viruses in leaves, vines and tubers were significantly (p < 0.05) higher in symptomatic plants than in asymptomatic plants. Some seed yams which were selected as apparently “clean” planting materials could harbour yam viruses in plant parts, other than the leaf which is often targeted by PST. |
| format | Article |
| id | doaj-art-6061e0edb5fd4a9bad6a3382a82ff3f4 |
| institution | Kabale University |
| issn | 2666-1543 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-6061e0edb5fd4a9bad6a3382a82ff3f42024-12-16T05:37:21ZengElsevierJournal of Agriculture and Food Research2666-15432024-12-0118101320Accumulation of yam viruses in different parts of white yam and the practice of positive selection technique in seed yam productionAtta Kwesi Aidoo0Richard Tuyee Awuah1Joseph Nee Lamptey2Marian Dorcas Quain3Allen Oppong4Ruth Naa Ashiokai Prempeh5Jakpasu Victor Kofi Afun6Esther Agyemang Marfo7Linda Appianimaa Abrokwah8CSIR-Crops Research Institute, P.O. Box 3785, Kumasi, Ghana; Corresponding author.Kwame Nkrumah University of Science and Technology, Private Mail Bag, KNUST, Kumasi, GhanaCSIR-Crops Research Institute, P.O. Box 3785, Kumasi, Ghana; Kwame Nkrumah University of Science and Technology, Private Mail Bag, KNUST, Kumasi, Ghana; CSIR- College of Science and Technology, Private Mail Bag, CSIR, Accra, GhanaCSIR-Crops Research Institute, P.O. Box 3785, Kumasi, Ghana; CSIR- College of Science and Technology, Private Mail Bag, CSIR, Accra, GhanaCSIR-Crops Research Institute, P.O. Box 3785, Kumasi, GhanaCSIR-Crops Research Institute, P.O. Box 3785, Kumasi, Ghana; CSIR- College of Science and Technology, Private Mail Bag, CSIR, Accra, GhanaKwame Nkrumah University of Science and Technology, Private Mail Bag, KNUST, Kumasi, GhanaCSIR-Crops Research Institute, P.O. Box 3785, Kumasi, GhanaCSIR-Crops Research Institute, P.O. Box 3785, Kumasi, GhanaThe success of Positive Selection Technique (PST) depends on the visual selection of virus-free plants, which may not always be possible due to virus accumulation in plant parts apart from the leaves. This study determined virus accumulations in different plant parts of white yam when practicing PST. Experimental plots were established at Ejura and Fumesua, and planted with planting materials (sourced from PST-practicing farmers) of three white yam varieties viz. Kpamyo, Dente and Pona. Symptomatic and asymptomatic plants from these fields were selected and yam mosaic virus (YMV), cucumber mosaic virus (CMV) and yam mild mosaic virus (YMMV) detected from their leaves, vines and tubers using DAS-ELISA. Virus concentration in the various plant organs were obtained from the absorbance values using BIO-RAD microplate reader. Disease incidence and severity were assessed at three months after planting. Data were subjected to ANOVA and means separated with LSD using the R software. In the subsequent season, harvested seed yams from symptomatic and asymptomatic plants were studied in the screenhouse and viruses detected in leaves and vines using RT-PCR. Plant viruses were found in all plant parts of the three white yam varieties. Concentration of all viruses in leaves, vines and tubers were significantly (p < 0.05) higher in symptomatic plants than in asymptomatic plants. Some seed yams which were selected as apparently “clean” planting materials could harbour yam viruses in plant parts, other than the leaf which is often targeted by PST.http://www.sciencedirect.com/science/article/pii/S2666154324003570DAS-ELISAPositive selection techniqueRT-PCRSeed yamYam virus |
| spellingShingle | Atta Kwesi Aidoo Richard Tuyee Awuah Joseph Nee Lamptey Marian Dorcas Quain Allen Oppong Ruth Naa Ashiokai Prempeh Jakpasu Victor Kofi Afun Esther Agyemang Marfo Linda Appianimaa Abrokwah Accumulation of yam viruses in different parts of white yam and the practice of positive selection technique in seed yam production Journal of Agriculture and Food Research DAS-ELISA Positive selection technique RT-PCR Seed yam Yam virus |
| title | Accumulation of yam viruses in different parts of white yam and the practice of positive selection technique in seed yam production |
| title_full | Accumulation of yam viruses in different parts of white yam and the practice of positive selection technique in seed yam production |
| title_fullStr | Accumulation of yam viruses in different parts of white yam and the practice of positive selection technique in seed yam production |
| title_full_unstemmed | Accumulation of yam viruses in different parts of white yam and the practice of positive selection technique in seed yam production |
| title_short | Accumulation of yam viruses in different parts of white yam and the practice of positive selection technique in seed yam production |
| title_sort | accumulation of yam viruses in different parts of white yam and the practice of positive selection technique in seed yam production |
| topic | DAS-ELISA Positive selection technique RT-PCR Seed yam Yam virus |
| url | http://www.sciencedirect.com/science/article/pii/S2666154324003570 |
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