Strategies for genetic manipulation of the halotolerant black yeast Hortaea werneckii: ectopic DNA integration and marker-free CRISPR/Cas9 transformation

Strategies for genetic manipulation of the halotolerant black yeast Hortaea werneckii: ectopic DNA integration and marker-free CRISPR/Cas9 transformation

ABSTRACT Hortaea werneckii is a halotolerant black yeast commonly found in hypersaline environments. This yeast is also the causative agent of tinea nigra, a superficial mycosis of the palm of the hand and soles of the feet of humans. In addition to their remarkable halotolerance, this black yeast e...

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Bibliographic Details
Main Authors: Yainitza Hernandez-Rodriguez, A. Makenzie Bullard, Rebecca J. Busch, Aidan Marshall, José M. Vargas-Muñiz
Format: Article
Language:English
Published: American Society for Microbiology 2025-01-01
Series:Microbiology Spectrum
Subjects:
Hortaea werneckii
transformation
morphology
ectopic integration
CRISPR/Cas9
Online Access:https://journals.asm.org/doi/10.1128/spectrum.02430-24
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