Transdermal electrophysiological recordings of diet-induced small fiber peripheral neuropathy using a needle electrode array in mice and man
BackgroundDiabetic peripheral neuropathy (DPN) is a common complication of diabetes. Proactive treatment options remain limited, which is exacerbated by a lack of sensitive and convenient diagnostics, especially early in disease progression or specifically to assess small fiber neuropathy (SFN), the...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1511383/full |
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| author | Magdalena Blaszkiewicz Magdalena Blaszkiewicz Lydia Caron Brooke Villinski Joshua Passarelli Julia M. Towne Naeemah M. Story Erin Merchant Furrukh S. Khan Nuri Emanetoglu Leonard Kass Rosemary L. Smith Kristy L. Townsend Kristy L. Townsend |
| author_facet | Magdalena Blaszkiewicz Magdalena Blaszkiewicz Lydia Caron Brooke Villinski Joshua Passarelli Julia M. Towne Naeemah M. Story Erin Merchant Furrukh S. Khan Nuri Emanetoglu Leonard Kass Rosemary L. Smith Kristy L. Townsend Kristy L. Townsend |
| author_sort | Magdalena Blaszkiewicz |
| collection | DOAJ |
| description | BackgroundDiabetic peripheral neuropathy (DPN) is a common complication of diabetes. Proactive treatment options remain limited, which is exacerbated by a lack of sensitive and convenient diagnostics, especially early in disease progression or specifically to assess small fiber neuropathy (SFN), the loss of distal small diameter axons that innervate tissues and organs.MethodsWe designed, fabricated, tested, and validated a first-of-its-kind medical diagnostic device for the functional assessment of transdermal small fiber nerve activity. This device, the Detecting Early Neuropathy (DEN), is an electrically conductive needle array designed to record nerve electrical activity in the skin and subdermal tissues, as a feature of a broader theragnostic platform.ResultsDEN recordings were validated across a time course of diet-induced PN in mice, using statistical and computational analyses and compared to other SFN measures. Based on these preclinical mouse data, the device design was adapted to obtain recordings in human with a flexible printed circuit board to mold to the leg or other skin regions. The DEN successfully recorded various types of neural activity in mouse and human, with or without stimulation, including validated action potentials and electromyography signals.ConclusionNew functional diagnostic tools like DEN offer a promising outlook for patients needing an earlier or more sensitive diagnosis of DPN/SFN, to allow for earlier and more effective treatment options, especially as more become available in the clinic in future years. |
| format | Article |
| id | doaj-art-5f7afb4f692c4a038b38b133c62ee68d |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-5f7afb4f692c4a038b38b133c62ee68d2025-01-10T06:10:48ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-01-011210.3389/fbioe.2024.15113831511383Transdermal electrophysiological recordings of diet-induced small fiber peripheral neuropathy using a needle electrode array in mice and manMagdalena Blaszkiewicz0Magdalena Blaszkiewicz1Lydia Caron2Brooke Villinski3Joshua Passarelli4Julia M. Towne5Naeemah M. Story6Erin Merchant7Furrukh S. Khan8Nuri Emanetoglu9Leonard Kass10Rosemary L. Smith11Kristy L. Townsend12Kristy L. Townsend13Department of Neurological Surgery, The Ohio State University, Columbus, OH, United StatesSchool of Biology and Ecology, University of Maine, Orono, ME, United StatesSchool of Biology and Ecology, University of Maine, Orono, ME, United StatesCollege of Engineering, University of Maine, Orono, ME, United StatesSchool of Biology and Ecology, University of Maine, Orono, ME, United StatesCollege of Engineering, University of Maine, Orono, ME, United StatesDepartment of Neurological Surgery, The Ohio State University, Columbus, OH, United StatesCollege of Engineering, University of Maine, Orono, ME, United StatesDepartment of Electrical and Computer Engineering, The Ohio State University, Columbus, OH, United StatesCollege of Engineering, University of Maine, Orono, ME, United StatesSchool of Biology and Ecology, University of Maine, Orono, ME, United StatesCollege of Engineering, University of Maine, Orono, ME, United StatesDepartment of Neurological Surgery, The Ohio State University, Columbus, OH, United StatesSchool of Biology and Ecology, University of Maine, Orono, ME, United StatesBackgroundDiabetic peripheral neuropathy (DPN) is a common complication of diabetes. Proactive treatment options remain limited, which is exacerbated by a lack of sensitive and convenient diagnostics, especially early in disease progression or specifically to assess small fiber neuropathy (SFN), the loss of distal small diameter axons that innervate tissues and organs.MethodsWe designed, fabricated, tested, and validated a first-of-its-kind medical diagnostic device for the functional assessment of transdermal small fiber nerve activity. This device, the Detecting Early Neuropathy (DEN), is an electrically conductive needle array designed to record nerve electrical activity in the skin and subdermal tissues, as a feature of a broader theragnostic platform.ResultsDEN recordings were validated across a time course of diet-induced PN in mice, using statistical and computational analyses and compared to other SFN measures. Based on these preclinical mouse data, the device design was adapted to obtain recordings in human with a flexible printed circuit board to mold to the leg or other skin regions. The DEN successfully recorded various types of neural activity in mouse and human, with or without stimulation, including validated action potentials and electromyography signals.ConclusionNew functional diagnostic tools like DEN offer a promising outlook for patients needing an earlier or more sensitive diagnosis of DPN/SFN, to allow for earlier and more effective treatment options, especially as more become available in the clinic in future years.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1511383/fullobesitymedical devicemicroneedle electrodesperipheral nervesdiabetic peripheral neuropathysmall fiber neuropathy |
| spellingShingle | Magdalena Blaszkiewicz Magdalena Blaszkiewicz Lydia Caron Brooke Villinski Joshua Passarelli Julia M. Towne Naeemah M. Story Erin Merchant Furrukh S. Khan Nuri Emanetoglu Leonard Kass Rosemary L. Smith Kristy L. Townsend Kristy L. Townsend Transdermal electrophysiological recordings of diet-induced small fiber peripheral neuropathy using a needle electrode array in mice and man Frontiers in Bioengineering and Biotechnology obesity medical device microneedle electrodes peripheral nerves diabetic peripheral neuropathy small fiber neuropathy |
| title | Transdermal electrophysiological recordings of diet-induced small fiber peripheral neuropathy using a needle electrode array in mice and man |
| title_full | Transdermal electrophysiological recordings of diet-induced small fiber peripheral neuropathy using a needle electrode array in mice and man |
| title_fullStr | Transdermal electrophysiological recordings of diet-induced small fiber peripheral neuropathy using a needle electrode array in mice and man |
| title_full_unstemmed | Transdermal electrophysiological recordings of diet-induced small fiber peripheral neuropathy using a needle electrode array in mice and man |
| title_short | Transdermal electrophysiological recordings of diet-induced small fiber peripheral neuropathy using a needle electrode array in mice and man |
| title_sort | transdermal electrophysiological recordings of diet induced small fiber peripheral neuropathy using a needle electrode array in mice and man |
| topic | obesity medical device microneedle electrodes peripheral nerves diabetic peripheral neuropathy small fiber neuropathy |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1511383/full |
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