Development of Alginate Composite Microparticles for Encapsulation of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i>
The probiotic bacterium <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> BB-12 (BB-12) was encapsulated in two composites, alginate/agar and alginate/agar/casein. The network structure and physicochemical properties of these composites are influenced by complex interactio...
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2024-11-01
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| author | Marko Vinceković Lana Živković Elmira Turkeyeva Botagoz Mutaliyeva Galiya Madybekova Suzana Šegota Nataša Šijaković Vujičić Anđela Pustak Tanja Jurkin Marta Kiš Sanja Kajić |
| author_facet | Marko Vinceković Lana Živković Elmira Turkeyeva Botagoz Mutaliyeva Galiya Madybekova Suzana Šegota Nataša Šijaković Vujičić Anđela Pustak Tanja Jurkin Marta Kiš Sanja Kajić |
| author_sort | Marko Vinceković |
| collection | DOAJ |
| description | The probiotic bacterium <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> BB-12 (BB-12) was encapsulated in two composites, alginate/agar and alginate/agar/casein. The network structure and physicochemical properties of these composites are influenced by complex interactions, including hydrogen bonding, electrostatic forces between biopolymers, calcium ions, and the encapsulated bacteria. The composites demonstrated a granular surface, with the granules being spatially oriented on the alginate/agar/BB-12 surface and linearly oriented on the alginate/agar/casein/BB-12 surface. They possess a highly organized microparticle structure and exhibit viscoelastic solid-like behavior. The alginate/agar/BB-12 composite showed higher storage modulus, shear stress, and shear strain values, indicating enhanced stability in various physical environments. Both composites displayed good thermal stability, aligning with their rheological properties, confirming their well-ordered structures. Despite differences in composite structures, the release mechanism of bacteria is governed by Fickian diffusion through the composite matrix. Based on physicochemical properties, the alginate/agar/casein composite is recommended for dairy product fermentation, while the alginate/agar composite seems more suitable for oral use. These findings provide new insights into the interactions between bacterial cultures and alginate composite ingredients. |
| format | Article |
| id | doaj-art-5f5ce2a0de0243d2a4bc2d80f86339d6 |
| institution | Kabale University |
| issn | 2310-2861 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj-art-5f5ce2a0de0243d2a4bc2d80f86339d62024-11-26T18:05:35ZengMDPI AGGels2310-28612024-11-01101175210.3390/gels10110752Development of Alginate Composite Microparticles for Encapsulation of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i>Marko Vinceković0Lana Živković1Elmira Turkeyeva2Botagoz Mutaliyeva3Galiya Madybekova4Suzana Šegota5Nataša Šijaković Vujičić6Anđela Pustak7Tanja Jurkin8Marta Kiš9Sanja Kajić10Division of Agroecology, Department of Chemistry, University of Zagreb Faculty of Agriculture, Svetošimunska 25, 10000 Zagreb, CroatiaDivision of Agroecology, Department of Chemistry, University of Zagreb Faculty of Agriculture, Svetošimunska 25, 10000 Zagreb, CroatiaBiotechnology Department, M. Auezov South-Kazakhstan University, Tauke-Khan, Shymkent 160000, KazakhstanBiotechnology Department, M. Auezov South-Kazakhstan University, Tauke-Khan, Shymkent 160000, KazakhstanChemistry Department, O. Zhanibekov South-Kazakhstan Pedagogical University, Baitursynov Street, 13, Shymkent 160000, KazakhstanLaboratory for Biocolloids and Surface Chemistry, Ruđer Bošković Institute, Bijenička c. 54, 10000 Zagreb, CroatiaDivision of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička c. 54, 10000 Zagreb, CroatiaRadiation Chemistry and Dosimetry Laboratory, Ruđer Bošković Institute, Bijenička c. 54, 10000 Zagreb, CroatiaRadiation Chemistry and Dosimetry Laboratory, Ruđer Bošković Institute, Bijenička c. 54, 10000 Zagreb, CroatiaFaculty of Veterinary Medicine, University of Zagreb, Ul. Vjekoslava Heinzela 55, 10000 Zagreb, CroatiaDivision of Agroecology, Department of Microbiology, University of Zagreb Faculty of Agriculture, 10000 Zagreb, CroatiaThe probiotic bacterium <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> BB-12 (BB-12) was encapsulated in two composites, alginate/agar and alginate/agar/casein. The network structure and physicochemical properties of these composites are influenced by complex interactions, including hydrogen bonding, electrostatic forces between biopolymers, calcium ions, and the encapsulated bacteria. The composites demonstrated a granular surface, with the granules being spatially oriented on the alginate/agar/BB-12 surface and linearly oriented on the alginate/agar/casein/BB-12 surface. They possess a highly organized microparticle structure and exhibit viscoelastic solid-like behavior. The alginate/agar/BB-12 composite showed higher storage modulus, shear stress, and shear strain values, indicating enhanced stability in various physical environments. Both composites displayed good thermal stability, aligning with their rheological properties, confirming their well-ordered structures. Despite differences in composite structures, the release mechanism of bacteria is governed by Fickian diffusion through the composite matrix. Based on physicochemical properties, the alginate/agar/casein composite is recommended for dairy product fermentation, while the alginate/agar composite seems more suitable for oral use. These findings provide new insights into the interactions between bacterial cultures and alginate composite ingredients.https://www.mdpi.com/2310-2861/10/11/752alginate compositeagarcasein<i>Bifidobacterium animalis</i> subsp. <i>lactis</i> BB-12encapsulation |
| spellingShingle | Marko Vinceković Lana Živković Elmira Turkeyeva Botagoz Mutaliyeva Galiya Madybekova Suzana Šegota Nataša Šijaković Vujičić Anđela Pustak Tanja Jurkin Marta Kiš Sanja Kajić Development of Alginate Composite Microparticles for Encapsulation of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> Gels alginate composite agar casein <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> BB-12 encapsulation |
| title | Development of Alginate Composite Microparticles for Encapsulation of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> |
| title_full | Development of Alginate Composite Microparticles for Encapsulation of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> |
| title_fullStr | Development of Alginate Composite Microparticles for Encapsulation of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> |
| title_full_unstemmed | Development of Alginate Composite Microparticles for Encapsulation of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> |
| title_short | Development of Alginate Composite Microparticles for Encapsulation of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> |
| title_sort | development of alginate composite microparticles for encapsulation of i bifidobacterium animalis i subsp i lactis i |
| topic | alginate composite agar casein <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> BB-12 encapsulation |
| url | https://www.mdpi.com/2310-2861/10/11/752 |
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