Understanding the regulation of aspartate metabolism using a model based on measured kinetic parameters
Abstract The aspartate‐derived amino‐acid pathway from plants is well suited for analysing the function of the allosteric network of interactions in branched pathways. For this purpose, a detailed kinetic model of the system in the plant model Arabidopsis was constructed on the basis of in vitro kin...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Nature
2009-05-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.1038/msb.2009.29 |
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| author | Gilles Curien Olivier Bastien Mylène Robert‐Genthon Athel Cornish‐Bowden María Luz Cárdenas Renaud Dumas |
| author_facet | Gilles Curien Olivier Bastien Mylène Robert‐Genthon Athel Cornish‐Bowden María Luz Cárdenas Renaud Dumas |
| author_sort | Gilles Curien |
| collection | DOAJ |
| description | Abstract The aspartate‐derived amino‐acid pathway from plants is well suited for analysing the function of the allosteric network of interactions in branched pathways. For this purpose, a detailed kinetic model of the system in the plant model Arabidopsis was constructed on the basis of in vitro kinetic measurements. The data, assembled into a mathematical model, reproduce in vivo measurements and also provide non‐intuitive predictions. A crucial result is the identification of allosteric interactions whose function is not to couple demand and supply but to maintain a high independence between fluxes in competing pathways. In addition, the model shows that enzyme isoforms are not functionally redundant, because they contribute unequally to the flux and its regulation. Another result is the identification of the threonine concentration as the most sensitive variable in the system, suggesting a regulatory role for threonine at a higher level of integration. |
| format | Article |
| id | doaj-art-5f11e262f7cf4f60b940ebd4e5c7828e |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2009-05-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-5f11e262f7cf4f60b940ebd4e5c7828e2025-08-24T11:58:51ZengSpringer NatureMolecular Systems Biology1744-42922009-05-015111410.1038/msb.2009.29Understanding the regulation of aspartate metabolism using a model based on measured kinetic parametersGilles Curien0Olivier Bastien1Mylène Robert‐Genthon2Athel Cornish‐Bowden3María Luz Cárdenas4Renaud Dumas5CNRS, UMR 5168, 17 rue des MartyrsINRA, UMR 1200CNRS, UMR 5168, 17 rue des MartyrsCNRS‐BIP, 31 chemin Joseph‐AiguierCNRS‐BIP, 31 chemin Joseph‐AiguierCNRS, UMR 5168, 17 rue des MartyrsAbstract The aspartate‐derived amino‐acid pathway from plants is well suited for analysing the function of the allosteric network of interactions in branched pathways. For this purpose, a detailed kinetic model of the system in the plant model Arabidopsis was constructed on the basis of in vitro kinetic measurements. The data, assembled into a mathematical model, reproduce in vivo measurements and also provide non‐intuitive predictions. A crucial result is the identification of allosteric interactions whose function is not to couple demand and supply but to maintain a high independence between fluxes in competing pathways. In addition, the model shows that enzyme isoforms are not functionally redundant, because they contribute unequally to the flux and its regulation. Another result is the identification of the threonine concentration as the most sensitive variable in the system, suggesting a regulatory role for threonine at a higher level of integration.https://doi.org/10.1038/msb.2009.29allosteric regulationArabidopsisaspartate metabolismmathematical modelsimulation |
| spellingShingle | Gilles Curien Olivier Bastien Mylène Robert‐Genthon Athel Cornish‐Bowden María Luz Cárdenas Renaud Dumas Understanding the regulation of aspartate metabolism using a model based on measured kinetic parameters Molecular Systems Biology allosteric regulation Arabidopsis aspartate metabolism mathematical model simulation |
| title | Understanding the regulation of aspartate metabolism using a model based on measured kinetic parameters |
| title_full | Understanding the regulation of aspartate metabolism using a model based on measured kinetic parameters |
| title_fullStr | Understanding the regulation of aspartate metabolism using a model based on measured kinetic parameters |
| title_full_unstemmed | Understanding the regulation of aspartate metabolism using a model based on measured kinetic parameters |
| title_short | Understanding the regulation of aspartate metabolism using a model based on measured kinetic parameters |
| title_sort | understanding the regulation of aspartate metabolism using a model based on measured kinetic parameters |
| topic | allosteric regulation Arabidopsis aspartate metabolism mathematical model simulation |
| url | https://doi.org/10.1038/msb.2009.29 |
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