Development of conventional PCR and a Real-Time PCR assay to choose the most suitable method for residual DNA identification of Beluga, Huso huso, in concrete fish ponds
A unique research was developed to achieve an effective and efficient method for tracing and identifying Beluga sturgeon species, Huso huso using eDNA in concrete fish ponds. Sampling was performed from the water of the Beluga pond and fixed with precipitation premix solution. DNA was extracted from...
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University of Guilan
2024-10-01
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| Series: | Caspian Journal of Environmental Sciences |
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| Online Access: | https://cjes.guilan.ac.ir/article_7984_7c31cff93a2502d1477a64e18bf224cb.pdf |
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| author | Esmaeil Noghanchi Sabet Shirin Jamshidi Ali Bani |
| author_facet | Esmaeil Noghanchi Sabet Shirin Jamshidi Ali Bani |
| author_sort | Esmaeil Noghanchi Sabet |
| collection | DOAJ |
| description | A unique research was developed to achieve an effective and efficient method for tracing and identifying Beluga sturgeon species, Huso huso using eDNA in concrete fish ponds. Sampling was performed from the water of the Beluga pond and fixed with precipitation premix solution. DNA was extracted from fixed water, and fin tissue of Beluga. Three short fragments were selected from a nuclear and two mitochondrial genes region of Beluga DNA. The specificity of primers was checked by conventional PCR for Beluga with fin tissue DNA samples. In this study, two conventional methods of molecular identification were performed on residual DNA samples of fish pond water and fin tissue, exhibiting that they were not suitable for measuring the residual DNA of Beluga in fish ponds due to the low rate of DNA. PCR amplification of sturgeon’s DNA of mitochondrial COΙ gene in third PCR primers was successful in low level of DNA extracted from water column and sediment by conventional and Quantitative PCR. The linear relationship between the threshold cycle (ct) value and Beluga DNA concentration was measured by the Mini-Barcoding quantitative Real-Time PCR. DNA samples of fish ponds generated a uniform curve in water column and sediment. In identifying residual DNA in the Picogram level of Beluga pond, SYBER Green Real-Time PCR method can be an acceptable method for barcoding with high efficiency compared to other methods such as conventional PCR method and non-invasive which is advantageous for the conservation of critically endangered sturgeons such as Beluga. This research results can also be used as a model for tracing fish species in rivers and seas. |
| format | Article |
| id | doaj-art-5efe06c92f3e47e3b40d3543624912f6 |
| institution | Kabale University |
| issn | 1735-3033 1735-3866 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | University of Guilan |
| record_format | Article |
| series | Caspian Journal of Environmental Sciences |
| spelling | doaj-art-5efe06c92f3e47e3b40d3543624912f62024-12-20T22:28:17ZengUniversity of GuilanCaspian Journal of Environmental Sciences1735-30331735-38662024-10-0122495596110.22124/cjes.2024.79847984Development of conventional PCR and a Real-Time PCR assay to choose the most suitable method for residual DNA identification of Beluga, Huso huso, in concrete fish pondsEsmaeil Noghanchi Sabet0Shirin Jamshidi1Ali Bani2Department of Biology, Faculty of Science, University of Guilan, Rasht, IranInternational Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, IranDepartment of Biology, Faculty of Science, University of Guilan, Rasht, IranA unique research was developed to achieve an effective and efficient method for tracing and identifying Beluga sturgeon species, Huso huso using eDNA in concrete fish ponds. Sampling was performed from the water of the Beluga pond and fixed with precipitation premix solution. DNA was extracted from fixed water, and fin tissue of Beluga. Three short fragments were selected from a nuclear and two mitochondrial genes region of Beluga DNA. The specificity of primers was checked by conventional PCR for Beluga with fin tissue DNA samples. In this study, two conventional methods of molecular identification were performed on residual DNA samples of fish pond water and fin tissue, exhibiting that they were not suitable for measuring the residual DNA of Beluga in fish ponds due to the low rate of DNA. PCR amplification of sturgeon’s DNA of mitochondrial COΙ gene in third PCR primers was successful in low level of DNA extracted from water column and sediment by conventional and Quantitative PCR. The linear relationship between the threshold cycle (ct) value and Beluga DNA concentration was measured by the Mini-Barcoding quantitative Real-Time PCR. DNA samples of fish ponds generated a uniform curve in water column and sediment. In identifying residual DNA in the Picogram level of Beluga pond, SYBER Green Real-Time PCR method can be an acceptable method for barcoding with high efficiency compared to other methods such as conventional PCR method and non-invasive which is advantageous for the conservation of critically endangered sturgeons such as Beluga. This research results can also be used as a model for tracing fish species in rivers and seas.https://cjes.guilan.ac.ir/article_7984_7c31cff93a2502d1477a64e18bf224cb.pdfbeluganuclear dnamitochondrial dnasyber green real-time pcr |
| spellingShingle | Esmaeil Noghanchi Sabet Shirin Jamshidi Ali Bani Development of conventional PCR and a Real-Time PCR assay to choose the most suitable method for residual DNA identification of Beluga, Huso huso, in concrete fish ponds Caspian Journal of Environmental Sciences beluga nuclear dna mitochondrial dna syber green real-time pcr |
| title | Development of conventional PCR and a Real-Time PCR assay to choose the most suitable method for residual DNA identification of Beluga, Huso huso, in concrete fish ponds |
| title_full | Development of conventional PCR and a Real-Time PCR assay to choose the most suitable method for residual DNA identification of Beluga, Huso huso, in concrete fish ponds |
| title_fullStr | Development of conventional PCR and a Real-Time PCR assay to choose the most suitable method for residual DNA identification of Beluga, Huso huso, in concrete fish ponds |
| title_full_unstemmed | Development of conventional PCR and a Real-Time PCR assay to choose the most suitable method for residual DNA identification of Beluga, Huso huso, in concrete fish ponds |
| title_short | Development of conventional PCR and a Real-Time PCR assay to choose the most suitable method for residual DNA identification of Beluga, Huso huso, in concrete fish ponds |
| title_sort | development of conventional pcr and a real time pcr assay to choose the most suitable method for residual dna identification of beluga huso huso in concrete fish ponds |
| topic | beluga nuclear dna mitochondrial dna syber green real-time pcr |
| url | https://cjes.guilan.ac.ir/article_7984_7c31cff93a2502d1477a64e18bf224cb.pdf |
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