Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells
Summary: Understanding metabolic conditions related to glycolysis dependence is crucial for developing new treatments in cancer and regenerative medicine. This protocol details a method for using the live-cell metabolic analyzer (LiCellMo) to measure continuous changes in glucose consumption and lac...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672400683X |
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| author | Kenji Miki Mikako Yagi Ko Igami Hiroki Kittaka Akito Tani Natsuki Horiuchi Satoshi Fukumoto Koji Yoshimoto Takeshi Uchiumi |
| author_facet | Kenji Miki Mikako Yagi Ko Igami Hiroki Kittaka Akito Tani Natsuki Horiuchi Satoshi Fukumoto Koji Yoshimoto Takeshi Uchiumi |
| author_sort | Kenji Miki |
| collection | DOAJ |
| description | Summary: Understanding metabolic conditions related to glycolysis dependence is crucial for developing new treatments in cancer and regenerative medicine. This protocol details a method for using the live-cell metabolic analyzer (LiCellMo) to measure continuous changes in glucose consumption and lactate production in cultured human cells. LiCellMo provides real-time data on consecutive metabolic changes, improving measurements of these processes in various contexts, including in cancer and regenerative treatments. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-5e5f68ac722c4a02aae73e70b874d44f |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-5e5f68ac722c4a02aae73e70b874d44f2025-01-12T05:25:46ZengElsevierSTAR Protocols2666-16672025-03-0161103518Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cellsKenji Miki0Mikako Yagi1Ko Igami2Hiroki Kittaka3Akito Tani4Natsuki Horiuchi5Satoshi Fukumoto6Koji Yoshimoto7Takeshi Uchiumi8Department of Health Sciences, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan; Department of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Health Sciences, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanKyushu Pro Search Limited Liability Partnership, Fukuoka, JapanKyushu Pro Search Limited Liability Partnership, Fukuoka, JapanDepartment of Health Sciences, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Health Sciences, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanPHC Corporation, Ehime, JapanDepartment of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Health Sciences, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan; Corresponding authorSummary: Understanding metabolic conditions related to glycolysis dependence is crucial for developing new treatments in cancer and regenerative medicine. This protocol details a method for using the live-cell metabolic analyzer (LiCellMo) to measure continuous changes in glucose consumption and lactate production in cultured human cells. LiCellMo provides real-time data on consecutive metabolic changes, improving measurements of these processes in various contexts, including in cancer and regenerative treatments. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672400683Xcell biologycell culturemetabolism |
| spellingShingle | Kenji Miki Mikako Yagi Ko Igami Hiroki Kittaka Akito Tani Natsuki Horiuchi Satoshi Fukumoto Koji Yoshimoto Takeshi Uchiumi Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells STAR Protocols cell biology cell culture metabolism |
| title | Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells |
| title_full | Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells |
| title_fullStr | Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells |
| title_full_unstemmed | Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells |
| title_short | Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells |
| title_sort | live cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells |
| topic | cell biology cell culture metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S266616672400683X |
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