Targeted lipidomics analysis of possible molecular mechanisms of lipid changes in temporal lobe epilepsy models
BackgroundLipids are vital biomolecules involved in the formation of various biofilms. Seizures can cause changes in lipid metabolism in the brain. In-depth studies at multiple levels are urgently needed to elucidate lipid composition, distribution, and metabolic pathways in the brain after seizure....
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1531524/full |
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| author | Huaiyu Sun Xuewei Li Zhiqing Chen Hongmei Meng |
| author_facet | Huaiyu Sun Xuewei Li Zhiqing Chen Hongmei Meng |
| author_sort | Huaiyu Sun |
| collection | DOAJ |
| description | BackgroundLipids are vital biomolecules involved in the formation of various biofilms. Seizures can cause changes in lipid metabolism in the brain. In-depth studies at multiple levels are urgently needed to elucidate lipid composition, distribution, and metabolic pathways in the brain after seizure.MethodsIn this research, a cutting-edge targeted quantitative lipidomics study was conducted on the hippocampal tissues of six rats with temporal lobe epilepsy and six normal rats. Accurate lipid quantification based on linear equations was calculated using an internal standard. The lipids were quantitatively and qualitatively analyzed by ultra-high performance liquid chromatography (UPLC) and mass spectrometry (MS).ResultsA total of 21 lipid classes were identified. Among them, the most abundant were triacylglycerol (TG), phosphatidyl ethanolamine (PE-P), and fatty acids (FA). Cholesteryl ester (ChE) exhibits the most considerable difference between the normal and epileptic samples. ChE was found to be the most significantly upregulated lipid, while FA was observed to be the most significantly downregulated lipid.ConclusionBased on the absolute quantitative analysis of lipids in rat hippocampal specimens, the contents and change trends of different lipids were observed. Upregulation of ChE and dihydroceramide (DHCer) was observed, and an analysis of the distribution changes elucidated the causes and possible molecular mechanisms of lipid accumulation in temporal lobe epilepsy. The results and methods described provide a comprehensive analysis of lipid metabolism in temporal lobe epilepsy and a new therapeutic target for the treatment of epilepsy. |
| format | Article |
| id | doaj-art-5e3ae3df2cc34af7a38e5f26196c312a |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj-art-5e3ae3df2cc34af7a38e5f26196c312a2025-01-09T06:10:09ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-01-011510.3389/fphar.2024.15315241531524Targeted lipidomics analysis of possible molecular mechanisms of lipid changes in temporal lobe epilepsy modelsHuaiyu Sun0Xuewei Li1Zhiqing Chen2Hongmei Meng3Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Radiology, The First Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Neurology, The First Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Neurology, The First Hospital of Jilin University, Changchun, Jilin, ChinaBackgroundLipids are vital biomolecules involved in the formation of various biofilms. Seizures can cause changes in lipid metabolism in the brain. In-depth studies at multiple levels are urgently needed to elucidate lipid composition, distribution, and metabolic pathways in the brain after seizure.MethodsIn this research, a cutting-edge targeted quantitative lipidomics study was conducted on the hippocampal tissues of six rats with temporal lobe epilepsy and six normal rats. Accurate lipid quantification based on linear equations was calculated using an internal standard. The lipids were quantitatively and qualitatively analyzed by ultra-high performance liquid chromatography (UPLC) and mass spectrometry (MS).ResultsA total of 21 lipid classes were identified. Among them, the most abundant were triacylglycerol (TG), phosphatidyl ethanolamine (PE-P), and fatty acids (FA). Cholesteryl ester (ChE) exhibits the most considerable difference between the normal and epileptic samples. ChE was found to be the most significantly upregulated lipid, while FA was observed to be the most significantly downregulated lipid.ConclusionBased on the absolute quantitative analysis of lipids in rat hippocampal specimens, the contents and change trends of different lipids were observed. Upregulation of ChE and dihydroceramide (DHCer) was observed, and an analysis of the distribution changes elucidated the causes and possible molecular mechanisms of lipid accumulation in temporal lobe epilepsy. The results and methods described provide a comprehensive analysis of lipid metabolism in temporal lobe epilepsy and a new therapeutic target for the treatment of epilepsy.https://www.frontiersin.org/articles/10.3389/fphar.2024.1531524/fulltargeted lipidomics analysisepilepsytemporal lobe epilepsymolecular mechanismtherapeutic target |
| spellingShingle | Huaiyu Sun Xuewei Li Zhiqing Chen Hongmei Meng Targeted lipidomics analysis of possible molecular mechanisms of lipid changes in temporal lobe epilepsy models Frontiers in Pharmacology targeted lipidomics analysis epilepsy temporal lobe epilepsy molecular mechanism therapeutic target |
| title | Targeted lipidomics analysis of possible molecular mechanisms of lipid changes in temporal lobe epilepsy models |
| title_full | Targeted lipidomics analysis of possible molecular mechanisms of lipid changes in temporal lobe epilepsy models |
| title_fullStr | Targeted lipidomics analysis of possible molecular mechanisms of lipid changes in temporal lobe epilepsy models |
| title_full_unstemmed | Targeted lipidomics analysis of possible molecular mechanisms of lipid changes in temporal lobe epilepsy models |
| title_short | Targeted lipidomics analysis of possible molecular mechanisms of lipid changes in temporal lobe epilepsy models |
| title_sort | targeted lipidomics analysis of possible molecular mechanisms of lipid changes in temporal lobe epilepsy models |
| topic | targeted lipidomics analysis epilepsy temporal lobe epilepsy molecular mechanism therapeutic target |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1531524/full |
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