Differential Biological Effects of <i>Trifolium pratense</i> Extracts—In Vitro Studies on Breast Cancer Models
The increasing popularity of herbal supplements emphasizes the need of scientific data regarding their health benefits and possible toxicological concerns. The complexity of botanical extracts, which include thousands of distinct compounds, contributes to the challenging nature of this endeavor. In...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/13/12/1435 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846106204727672832 |
|---|---|
| author | Lucian Albulescu Alexandru Suciu Mihaela Neagu Cristiana Tanase Sevinci Pop |
| author_facet | Lucian Albulescu Alexandru Suciu Mihaela Neagu Cristiana Tanase Sevinci Pop |
| author_sort | Lucian Albulescu |
| collection | DOAJ |
| description | The increasing popularity of herbal supplements emphasizes the need of scientific data regarding their health benefits and possible toxicological concerns. The complexity of botanical extracts, which include thousands of distinct compounds, contributes to the challenging nature of this endeavor. In this study, we explored the hormetic effects of two <i>Trifolium pratense</i> extracts on breast cell lines. Using a wide range of concentrations (0.1 to 3.33 mg/mL), we analyzed how extracts modulate cellular processes such as viability, proliferation, and oxidative stress on breast adenocarcinoma highly invasive estrogen receptor negative (ER-) and noninvasive ER+ cells, as well as on non-tumorigenic ER- normal cells. The cytotoxicity and real-time cell analysis (RTCA) assays showed that both extracts exercised a biphasic dose effect on adenocarcinoma ER+ and normal ER- cell proliferation and oxidative stress. We report a monotonic dose-dependent cytotoxicity on highly invasive adenocarcinoma ER- cells; the induced apoptosis was based on the pro-oxidant activity of extracts. The reactive oxygen species (ROS) generation by high-dose ethanolic extract was observed in all cells, followed by mitochondria dysfunction. Oxidative stress parameters, such as malondialdehyde (MDA) and reduced glutathione (GSH) levels, and superoxide dismutase (SOD) activity were affected. Our study demonstrates that <i>T. pratense</i> extracts have chemoprevention potential in normal and tumorigenic breast cells by modulating cellular proliferation and oxidative stress. |
| format | Article |
| id | doaj-art-5e1a64e63dc042959c36c81a637a65e8 |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-5e1a64e63dc042959c36c81a637a65e82024-12-27T14:06:34ZengMDPI AGAntioxidants2076-39212024-11-011312143510.3390/antiox13121435Differential Biological Effects of <i>Trifolium pratense</i> Extracts—In Vitro Studies on Breast Cancer ModelsLucian Albulescu0Alexandru Suciu1Mihaela Neagu2Cristiana Tanase3Sevinci Pop4Biochemistry & Proteomics Laboratory, “Victor Babes” National Institute of Pathology, 050096 Bucharest, RomaniaResearch Department, SC Hofigal Export-Import SA, 042124 Bucharest, RomaniaResearch Department, SC Hofigal Export-Import SA, 042124 Bucharest, RomaniaBiochemistry & Proteomics Laboratory, “Victor Babes” National Institute of Pathology, 050096 Bucharest, RomaniaCell Biology Laboratory, “Victor Babes” National Institute of Pathology, 050096 Bucharest, RomaniaThe increasing popularity of herbal supplements emphasizes the need of scientific data regarding their health benefits and possible toxicological concerns. The complexity of botanical extracts, which include thousands of distinct compounds, contributes to the challenging nature of this endeavor. In this study, we explored the hormetic effects of two <i>Trifolium pratense</i> extracts on breast cell lines. Using a wide range of concentrations (0.1 to 3.33 mg/mL), we analyzed how extracts modulate cellular processes such as viability, proliferation, and oxidative stress on breast adenocarcinoma highly invasive estrogen receptor negative (ER-) and noninvasive ER+ cells, as well as on non-tumorigenic ER- normal cells. The cytotoxicity and real-time cell analysis (RTCA) assays showed that both extracts exercised a biphasic dose effect on adenocarcinoma ER+ and normal ER- cell proliferation and oxidative stress. We report a monotonic dose-dependent cytotoxicity on highly invasive adenocarcinoma ER- cells; the induced apoptosis was based on the pro-oxidant activity of extracts. The reactive oxygen species (ROS) generation by high-dose ethanolic extract was observed in all cells, followed by mitochondria dysfunction. Oxidative stress parameters, such as malondialdehyde (MDA) and reduced glutathione (GSH) levels, and superoxide dismutase (SOD) activity were affected. Our study demonstrates that <i>T. pratense</i> extracts have chemoprevention potential in normal and tumorigenic breast cells by modulating cellular proliferation and oxidative stress.https://www.mdpi.com/2076-3921/13/12/1435red cloverplant extractshormeticbiphasic dose-response effectschemopreventionoxidative stress |
| spellingShingle | Lucian Albulescu Alexandru Suciu Mihaela Neagu Cristiana Tanase Sevinci Pop Differential Biological Effects of <i>Trifolium pratense</i> Extracts—In Vitro Studies on Breast Cancer Models Antioxidants red clover plant extracts hormetic biphasic dose-response effects chemoprevention oxidative stress |
| title | Differential Biological Effects of <i>Trifolium pratense</i> Extracts—In Vitro Studies on Breast Cancer Models |
| title_full | Differential Biological Effects of <i>Trifolium pratense</i> Extracts—In Vitro Studies on Breast Cancer Models |
| title_fullStr | Differential Biological Effects of <i>Trifolium pratense</i> Extracts—In Vitro Studies on Breast Cancer Models |
| title_full_unstemmed | Differential Biological Effects of <i>Trifolium pratense</i> Extracts—In Vitro Studies on Breast Cancer Models |
| title_short | Differential Biological Effects of <i>Trifolium pratense</i> Extracts—In Vitro Studies on Breast Cancer Models |
| title_sort | differential biological effects of i trifolium pratense i extracts in vitro studies on breast cancer models |
| topic | red clover plant extracts hormetic biphasic dose-response effects chemoprevention oxidative stress |
| url | https://www.mdpi.com/2076-3921/13/12/1435 |
| work_keys_str_mv | AT lucianalbulescu differentialbiologicaleffectsofitrifoliumpratenseiextractsinvitrostudiesonbreastcancermodels AT alexandrusuciu differentialbiologicaleffectsofitrifoliumpratenseiextractsinvitrostudiesonbreastcancermodels AT mihaelaneagu differentialbiologicaleffectsofitrifoliumpratenseiextractsinvitrostudiesonbreastcancermodels AT cristianatanase differentialbiologicaleffectsofitrifoliumpratenseiextractsinvitrostudiesonbreastcancermodels AT sevincipop differentialbiologicaleffectsofitrifoliumpratenseiextractsinvitrostudiesonbreastcancermodels |