An In Vivo Model of Estrogen Supplementation Concerning the Expression of Ca<sup>2+</sup>-Dependent Exchangers and Mortality, Vitality and Survival After Myocardial Infarction in Ovariectomized Rats
Background: Ischemic-reperfusion damage of cardiomyocytes due to myocardial infarction (MI) often leads to the death of an individual. Premenopausal women have been observed to have a significantly lower risk of cardiovascular disease (CVD) than men of the same age. In menopausal women, this trend i...
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2024-11-01
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| author | Tomáš Toporcer Tomáš Grendel Ivana Špaková Alžbeta Blichárová Ľudmila Verbóová Zuzana Benetinová Beata Čižmárová Miroslava Rabajdová Silvia Toporcerová |
| author_facet | Tomáš Toporcer Tomáš Grendel Ivana Špaková Alžbeta Blichárová Ľudmila Verbóová Zuzana Benetinová Beata Čižmárová Miroslava Rabajdová Silvia Toporcerová |
| author_sort | Tomáš Toporcer |
| collection | DOAJ |
| description | Background: Ischemic-reperfusion damage of cardiomyocytes due to myocardial infarction (MI) often leads to the death of an individual. Premenopausal women have been observed to have a significantly lower risk of cardiovascular disease (CVD) than men of the same age. In menopausal women, this trend is significantly reversed, and the risk of CVD increases up to 10-fold. Estrogens affect the development and function of the heart muscle, and as they decrease, the risk and poor prognosis of CVD increase. This study is focused on the effects of estrogen supplementation on morbidity, vitality, and <i>NCX1</i> expression after MI on a model system. Methods: In this study, female Sprague Dawley rats (n = 58), which were divided into three experimental groups (NN—control group, non-supplemented; OVX-N—ovariectomized, non-supplemented; OVX-S—ovariectomized, supplemented), received left thoracotomy in the fourth intercostal space. The left anterior descendent coronary artery was ligated 2 mm from its origin with an 8.0 suture. An immunohistological analysis as well as an RT-PCR analysis of <i>NCX1</i> expression were performed. Results: A higher survival rate was recorded in the OVX-N group (86%) in comparison with the OVX-S group (53%) (<i>p</i> < 0.05). In addition, higher <i>NCX1</i> expression 7 days/14 days after MI in the OVX-S group in comparison with the NN and OVX-N (<i>p</i> < 0.001 and <i>p</i> < 0.05) groups was recorded. Seven days after MI, a significantly higher expression (<i>p</i> < 0.005) of mRNA <i>NCX1</i> in the OVX-N group was also recorded in comparison with the NN group. Conclusions: This study provides a comprehensive description of the effect of estrogen supplementation on <i>NCX1</i> expression and overall vitality in ovariectomized rats that survived MI. |
| format | Article |
| id | doaj-art-5e172d6055b44ea9af40ef5d2c3014ae |
| institution | Kabale University |
| issn | 2308-3425 |
| language | English |
| publishDate | 2024-11-01 |
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| series | Journal of Cardiovascular Development and Disease |
| spelling | doaj-art-5e172d6055b44ea9af40ef5d2c3014ae2024-11-26T18:07:21ZengMDPI AGJournal of Cardiovascular Development and Disease2308-34252024-11-01111135210.3390/jcdd11110352An In Vivo Model of Estrogen Supplementation Concerning the Expression of Ca<sup>2+</sup>-Dependent Exchangers and Mortality, Vitality and Survival After Myocardial Infarction in Ovariectomized RatsTomáš Toporcer0Tomáš Grendel1Ivana Špaková2Alžbeta Blichárová3Ľudmila Verbóová4Zuzana Benetinová5Beata Čižmárová6Miroslava Rabajdová7Silvia Toporcerová8Department of Heart Surgery, East Slovak Institute of Cardiovascular Disease and Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Košice, SlovakiaDepartment of Anesthesiology and Intensive Medicine, East Slovak Institute of Cardiovascular Disease and Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Košice, SlovakiaDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Košice, SlovakiaDepartment of Pathology, Louis Pasteur University Hospital and Faculty of Medicine, Pavol Jozef Šafárik University, 040 01 Košice, SlovakiaDepartment of Pathology, Louis Pasteur University Hospital and Faculty of Medicine, Pavol Jozef Šafárik University, 040 01 Košice, SlovakiaDepartment of Pathology, Louis Pasteur University Hospital and Faculty of Medicine, Pavol Jozef Šafárik University, 040 01 Košice, SlovakiaDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Košice, SlovakiaDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Košice, SlovakiaDepartment of Gynecology and Obstetrics, Faculty of Medicine, Pavol Jozef Šafárik University and Gyncare, 040 11 Košice, SlovakiaBackground: Ischemic-reperfusion damage of cardiomyocytes due to myocardial infarction (MI) often leads to the death of an individual. Premenopausal women have been observed to have a significantly lower risk of cardiovascular disease (CVD) than men of the same age. In menopausal women, this trend is significantly reversed, and the risk of CVD increases up to 10-fold. Estrogens affect the development and function of the heart muscle, and as they decrease, the risk and poor prognosis of CVD increase. This study is focused on the effects of estrogen supplementation on morbidity, vitality, and <i>NCX1</i> expression after MI on a model system. Methods: In this study, female Sprague Dawley rats (n = 58), which were divided into three experimental groups (NN—control group, non-supplemented; OVX-N—ovariectomized, non-supplemented; OVX-S—ovariectomized, supplemented), received left thoracotomy in the fourth intercostal space. The left anterior descendent coronary artery was ligated 2 mm from its origin with an 8.0 suture. An immunohistological analysis as well as an RT-PCR analysis of <i>NCX1</i> expression were performed. Results: A higher survival rate was recorded in the OVX-N group (86%) in comparison with the OVX-S group (53%) (<i>p</i> < 0.05). In addition, higher <i>NCX1</i> expression 7 days/14 days after MI in the OVX-S group in comparison with the NN and OVX-N (<i>p</i> < 0.001 and <i>p</i> < 0.05) groups was recorded. Seven days after MI, a significantly higher expression (<i>p</i> < 0.005) of mRNA <i>NCX1</i> in the OVX-N group was also recorded in comparison with the NN group. Conclusions: This study provides a comprehensive description of the effect of estrogen supplementation on <i>NCX1</i> expression and overall vitality in ovariectomized rats that survived MI.https://www.mdpi.com/2308-3425/11/11/352myocardial infarctionrhythm disordersestrogen supplementation<i>NCX1</i>ovariectomized rat |
| spellingShingle | Tomáš Toporcer Tomáš Grendel Ivana Špaková Alžbeta Blichárová Ľudmila Verbóová Zuzana Benetinová Beata Čižmárová Miroslava Rabajdová Silvia Toporcerová An In Vivo Model of Estrogen Supplementation Concerning the Expression of Ca<sup>2+</sup>-Dependent Exchangers and Mortality, Vitality and Survival After Myocardial Infarction in Ovariectomized Rats Journal of Cardiovascular Development and Disease myocardial infarction rhythm disorders estrogen supplementation <i>NCX1</i> ovariectomized rat |
| title | An In Vivo Model of Estrogen Supplementation Concerning the Expression of Ca<sup>2+</sup>-Dependent Exchangers and Mortality, Vitality and Survival After Myocardial Infarction in Ovariectomized Rats |
| title_full | An In Vivo Model of Estrogen Supplementation Concerning the Expression of Ca<sup>2+</sup>-Dependent Exchangers and Mortality, Vitality and Survival After Myocardial Infarction in Ovariectomized Rats |
| title_fullStr | An In Vivo Model of Estrogen Supplementation Concerning the Expression of Ca<sup>2+</sup>-Dependent Exchangers and Mortality, Vitality and Survival After Myocardial Infarction in Ovariectomized Rats |
| title_full_unstemmed | An In Vivo Model of Estrogen Supplementation Concerning the Expression of Ca<sup>2+</sup>-Dependent Exchangers and Mortality, Vitality and Survival After Myocardial Infarction in Ovariectomized Rats |
| title_short | An In Vivo Model of Estrogen Supplementation Concerning the Expression of Ca<sup>2+</sup>-Dependent Exchangers and Mortality, Vitality and Survival After Myocardial Infarction in Ovariectomized Rats |
| title_sort | in vivo model of estrogen supplementation concerning the expression of ca sup 2 sup dependent exchangers and mortality vitality and survival after myocardial infarction in ovariectomized rats |
| topic | myocardial infarction rhythm disorders estrogen supplementation <i>NCX1</i> ovariectomized rat |
| url | https://www.mdpi.com/2308-3425/11/11/352 |
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