OP-6 EVALUATION OF THE HEPATOPROTECTIVE ACTIVITY OF Flourensia cernua AND ITS IMPACT ON THE AEROBIC INTESTINAL MICROBIOTA IN A VALPROIC ACID-INDUCED DAMAGE MODEL IN WISTAR RATS
Conflict of interest: No Introduction and Objectives: Liver disease is a health problem that accounts for more than 2 million deaths per year worldwide. Valproic acid (VPA) has been used as a hepatotoxic agent in animal models to reproduce liver damage and test future therapeutic strategies. Flouren...
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Elsevier
2024-12-01
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| Series: | Annals of Hepatology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1665268124003879 |
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| author | Liliana Torres González José Miguel De la Rosa García Jorge Martín Llaca Díaz Lorena Salazar Cavazos Humberto Rodríguez Rocha Aracely García García Catalina Leos Rivas Veronica Mayela Rivas Galindo Luis Alejandro Pérez López Diana Patricia Moreno Peña Diana Raquel Rodríguez Rodríguez Linda E. Muñoz Espinosa Paula Cordero Pérez |
| author_facet | Liliana Torres González José Miguel De la Rosa García Jorge Martín Llaca Díaz Lorena Salazar Cavazos Humberto Rodríguez Rocha Aracely García García Catalina Leos Rivas Veronica Mayela Rivas Galindo Luis Alejandro Pérez López Diana Patricia Moreno Peña Diana Raquel Rodríguez Rodríguez Linda E. Muñoz Espinosa Paula Cordero Pérez |
| author_sort | Liliana Torres González |
| collection | DOAJ |
| description | Conflict of interest: No Introduction and Objectives: Liver disease is a health problem that accounts for more than 2 million deaths per year worldwide. Valproic acid (VPA) has been used as a hepatotoxic agent in animal models to reproduce liver damage and test future therapeutic strategies. Flourensia cernua (Fc) is a plant that contains compounds with antioxidant activity, which may have a potential hepatoprotective effect.The aim was to evaluate the hepatoprotective activity of Flourensia cernua and its impact on aerobic intestinal microbiota in a valproic acid-induced damage model in Wistar rats. Patients / Materials and Methods: Seven groups were used (n=6): 1) Sham, 2) VPA, 500 mg/kg of VPA/d/7d i.p., 3) Fc extract at 200 mg/kg/d/3d p.o., 4) Fc extract at 400 mg/kg/d/3d p.o., 5) VPA + Fc 200 mg/kg/d/3d p.o., 6) VPA + Fc 400 mg/kg/d/3d p.o., 7) VPA + Silibinin 200 mg/kg/d/3d p.o.; subsequently, the animals were sacrificed, and samples of faeces, blood, and liver tissue were taken for aerobic intestinal microbiota(AIM), biochemical markers, oxidative stress, and histological analysis, respectively. Data was analyzed using Prism software (v. 10.0.0; GraphPad). P<0.05 was statistically significant. Results and Discussion: VPA significantly increased ALT, AST and decreased total proteins vs. Sham. There was no alteration of transaminases at both tested doses of Fc extract. Only the VPA + Fc 400 mg/kg group showed a significant reduction in ALT, AST, SOD, and MDA vs. VPA, similar to silibinin. The histological analysis did not show significant changes in the study groups. MALDI-TOF primarily identified Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, and Ochrobactrum intermedium as AIM in the different study groups. Conclusions: The hydroalcoholic extract of F. cernua did not show toxicity at the evaluated doses, showed a hepatoprotective effect at 400 mg/kg, and did not modify AIM. VPA decreased AIM, and F. cernua showed a trend to partially restore the normal bacterial count, similar to silibinin. |
| format | Article |
| id | doaj-art-5df8c2bbc2c04c01b0aaf65ceb8e7449 |
| institution | Kabale University |
| issn | 1665-2681 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Annals of Hepatology |
| spelling | doaj-art-5df8c2bbc2c04c01b0aaf65ceb8e74492024-12-07T08:25:20ZengElsevierAnnals of Hepatology1665-26812024-12-0129101604OP-6 EVALUATION OF THE HEPATOPROTECTIVE ACTIVITY OF Flourensia cernua AND ITS IMPACT ON THE AEROBIC INTESTINAL MICROBIOTA IN A VALPROIC ACID-INDUCED DAMAGE MODEL IN WISTAR RATSLiliana Torres González0José Miguel De la Rosa García1Jorge Martín Llaca Díaz2Lorena Salazar Cavazos3Humberto Rodríguez Rocha4Aracely García García5Catalina Leos Rivas6Veronica Mayela Rivas Galindo7Luis Alejandro Pérez López8Diana Patricia Moreno Peña9Diana Raquel Rodríguez Rodríguez10Linda E. Muñoz Espinosa11Paula Cordero Pérez12Liver Unit, Department of Internal Medicine, University Hospital ''Dr. José E. González'', Universidad Autónoma de Nuevo León, Monterrey 64460, MexicoLiver Unit, Department of Internal Medicine, University Hospital ''Dr. José E. González'', Universidad Autónoma de Nuevo León, Monterrey 64460, MexicoDeparment of Clinical Pathology, University Hospital ''Dr. José E. González'', Universidad Autónoma de Nuevo León, Monterrey 64460, MexicoDeparment of Clinical Pathology, University Hospital ''Dr. José E. González'', Universidad Autónoma de Nuevo León, Monterrey 64460, MexicoDeparment of Histology, Faculty of Medicine, Universidad Autónoma de Nuevo León, Monterrey 64460, MexicoDeparment of Histology, Faculty of Medicine, Universidad Autónoma de Nuevo León, Monterrey 64460, MexicoDeparment of Chemistry, Faculty of Biological Sciences, Universidad Autónoma de Nuevo León, Monterrey 64460, MexicoDepartment of Analytical Chemistry, Faculty of Medicine, Universidad Autónoma de Nuevo León, Monterrey 64460, MexicoDepartment of Analytical Chemistry, Faculty of Medicine, Universidad Autónoma de Nuevo León, Monterrey 64460, MexicoLiver Unit, Department of Internal Medicine, University Hospital ''Dr. José E. González'', Universidad Autónoma de Nuevo León, Monterrey 64460, MexicoLiver Unit, Department of Internal Medicine, University Hospital ''Dr. José E. González'', Universidad Autónoma de Nuevo León, Monterrey 64460, MexicoLiver Unit, Department of Internal Medicine, University Hospital ''Dr. José E. González'', Universidad Autónoma de Nuevo León, Monterrey 64460, MexicoLiver Unit, Department of Internal Medicine, University Hospital ''Dr. José E. González'', Universidad Autónoma de Nuevo León, Monterrey 64460, MexicoConflict of interest: No Introduction and Objectives: Liver disease is a health problem that accounts for more than 2 million deaths per year worldwide. Valproic acid (VPA) has been used as a hepatotoxic agent in animal models to reproduce liver damage and test future therapeutic strategies. Flourensia cernua (Fc) is a plant that contains compounds with antioxidant activity, which may have a potential hepatoprotective effect.The aim was to evaluate the hepatoprotective activity of Flourensia cernua and its impact on aerobic intestinal microbiota in a valproic acid-induced damage model in Wistar rats. Patients / Materials and Methods: Seven groups were used (n=6): 1) Sham, 2) VPA, 500 mg/kg of VPA/d/7d i.p., 3) Fc extract at 200 mg/kg/d/3d p.o., 4) Fc extract at 400 mg/kg/d/3d p.o., 5) VPA + Fc 200 mg/kg/d/3d p.o., 6) VPA + Fc 400 mg/kg/d/3d p.o., 7) VPA + Silibinin 200 mg/kg/d/3d p.o.; subsequently, the animals were sacrificed, and samples of faeces, blood, and liver tissue were taken for aerobic intestinal microbiota(AIM), biochemical markers, oxidative stress, and histological analysis, respectively. Data was analyzed using Prism software (v. 10.0.0; GraphPad). P<0.05 was statistically significant. Results and Discussion: VPA significantly increased ALT, AST and decreased total proteins vs. Sham. There was no alteration of transaminases at both tested doses of Fc extract. Only the VPA + Fc 400 mg/kg group showed a significant reduction in ALT, AST, SOD, and MDA vs. VPA, similar to silibinin. The histological analysis did not show significant changes in the study groups. MALDI-TOF primarily identified Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, and Ochrobactrum intermedium as AIM in the different study groups. Conclusions: The hydroalcoholic extract of F. cernua did not show toxicity at the evaluated doses, showed a hepatoprotective effect at 400 mg/kg, and did not modify AIM. VPA decreased AIM, and F. cernua showed a trend to partially restore the normal bacterial count, similar to silibinin.http://www.sciencedirect.com/science/article/pii/S1665268124003879 |
| spellingShingle | Liliana Torres González José Miguel De la Rosa García Jorge Martín Llaca Díaz Lorena Salazar Cavazos Humberto Rodríguez Rocha Aracely García García Catalina Leos Rivas Veronica Mayela Rivas Galindo Luis Alejandro Pérez López Diana Patricia Moreno Peña Diana Raquel Rodríguez Rodríguez Linda E. Muñoz Espinosa Paula Cordero Pérez OP-6 EVALUATION OF THE HEPATOPROTECTIVE ACTIVITY OF Flourensia cernua AND ITS IMPACT ON THE AEROBIC INTESTINAL MICROBIOTA IN A VALPROIC ACID-INDUCED DAMAGE MODEL IN WISTAR RATS Annals of Hepatology |
| title | OP-6 EVALUATION OF THE HEPATOPROTECTIVE ACTIVITY OF Flourensia cernua AND ITS IMPACT ON THE AEROBIC INTESTINAL MICROBIOTA IN A VALPROIC ACID-INDUCED DAMAGE MODEL IN WISTAR RATS |
| title_full | OP-6 EVALUATION OF THE HEPATOPROTECTIVE ACTIVITY OF Flourensia cernua AND ITS IMPACT ON THE AEROBIC INTESTINAL MICROBIOTA IN A VALPROIC ACID-INDUCED DAMAGE MODEL IN WISTAR RATS |
| title_fullStr | OP-6 EVALUATION OF THE HEPATOPROTECTIVE ACTIVITY OF Flourensia cernua AND ITS IMPACT ON THE AEROBIC INTESTINAL MICROBIOTA IN A VALPROIC ACID-INDUCED DAMAGE MODEL IN WISTAR RATS |
| title_full_unstemmed | OP-6 EVALUATION OF THE HEPATOPROTECTIVE ACTIVITY OF Flourensia cernua AND ITS IMPACT ON THE AEROBIC INTESTINAL MICROBIOTA IN A VALPROIC ACID-INDUCED DAMAGE MODEL IN WISTAR RATS |
| title_short | OP-6 EVALUATION OF THE HEPATOPROTECTIVE ACTIVITY OF Flourensia cernua AND ITS IMPACT ON THE AEROBIC INTESTINAL MICROBIOTA IN A VALPROIC ACID-INDUCED DAMAGE MODEL IN WISTAR RATS |
| title_sort | op 6 evaluation of the hepatoprotective activity of flourensia cernua and its impact on the aerobic intestinal microbiota in a valproic acid induced damage model in wistar rats |
| url | http://www.sciencedirect.com/science/article/pii/S1665268124003879 |
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