Spectroscopy Technologies to Screen Peanut Seeds with Superior Vigor Through “Chemical Fingerprinting”
Peanut seeds are harvested at different development stages (early and late) due to their uneven maturation. At the time of harvest, approximately 30% of the seeds are still immature, meaning they are not completely filled with compounds (e.g., oil and minerals) and exhibit reduced vigor. Hypothetica...
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MDPI AG
2024-10-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/14/11/2529 |
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| author | Gustavo Roberto Fonseca de Oliveira Welinton Yoshio Hirai Dennis Silva Ferreira Karolyne Priscila Oliveira Mota da Silva Giovani Chaves Silva Tiago Bueno Moraes Clissia Barboza Mastrangelo Fabiola Manhas Verbi Pereira Edenir Rodrigues Pereira-Filho Edvaldo Aparecido Amaral da Silva |
| author_facet | Gustavo Roberto Fonseca de Oliveira Welinton Yoshio Hirai Dennis Silva Ferreira Karolyne Priscila Oliveira Mota da Silva Giovani Chaves Silva Tiago Bueno Moraes Clissia Barboza Mastrangelo Fabiola Manhas Verbi Pereira Edenir Rodrigues Pereira-Filho Edvaldo Aparecido Amaral da Silva |
| author_sort | Gustavo Roberto Fonseca de Oliveira |
| collection | DOAJ |
| description | Peanut seeds are harvested at different development stages (early and late) due to their uneven maturation. At the time of harvest, approximately 30% of the seeds are still immature, meaning they are not completely filled with compounds (e.g., oil and minerals) and exhibit reduced vigor. Hypothetically, these compounds can be detected as a “chemical fingerprinting” to classify seed maturation stages. Here, we investigated whether non-destructive techniques such as benchtop nuclear magnetic resonance (NMR), laser-induced breakdown spectroscopy (LIBS), and energy-dispersive X-ray fluorescence (ED-XRF) can identify chemical patterns unique to mature seeds with superior vigor. Field-grown seeds were classified into early (R5 and R6) and late (R7, R8, and R9) stages. Seed weight, germination, vigor, H<sub>2</sub>O<sub>2</sub>, and MDA (oxidative stress) were analyzed. Oil, potassium (K), and calcium (Ca) were measured digitally using spectroscopy techniques. We found that: (i) oxidative stress and K levels were higher in seeds from the early stages; (ii) seed oil and Ca were proportional to high-vigor seedlings and successful plant establishment in the field; and (iii) the seed chemical composition could be identified autonomously with 87% to 100% accuracy. In conclusion, LIBS, ED-XRF, and NMR technologies can effectively screen peanut seeds with superior vigor through “chemical fingerprinting”. |
| format | Article |
| id | doaj-art-5de5be47d7fc4a42b8baec204da26f94 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-5de5be47d7fc4a42b8baec204da26f942024-11-26T17:44:20ZengMDPI AGAgronomy2073-43952024-10-011411252910.3390/agronomy14112529Spectroscopy Technologies to Screen Peanut Seeds with Superior Vigor Through “Chemical Fingerprinting”Gustavo Roberto Fonseca de Oliveira0Welinton Yoshio Hirai1Dennis Silva Ferreira2Karolyne Priscila Oliveira Mota da Silva3Giovani Chaves Silva4Tiago Bueno Moraes5Clissia Barboza Mastrangelo6Fabiola Manhas Verbi Pereira7Edenir Rodrigues Pereira-Filho8Edvaldo Aparecido Amaral da Silva9Department of Crop Science, School of Agricultural Sciences, São Paulo State University, Botucatu 18610-034, SP, BrazilDepartment of Exacts Sciences, College of Agriculture “Luiz de Queiroz”, University of São Paulo, Piracicaba 13418-900, SP, BrazilGroup of Analytical Instrumental Analysis, Chemistry Department, Federal University of São Carlos, São Carlos 13565-905, SP, BrazilDepartment of Crop Science, School of Agricultural Sciences, São Paulo State University, Botucatu 18610-034, SP, BrazilGroup of Alternative Analytical Approaches, Bioenergy Research Institute, Institute of Chemistry, São Paulo State University, Araraquara 14800-060, SP, BrazilDepartment of Biosystems Engineering, College of Agriculture “Luiz de Queiroz”, University of São Paulo (USP), Piracicaba 13418-900, SP, BrazilLaboratory of Radiobiology and Environment, Center for Nuclear Energy in Agriculture, University of São Paulo, Piracicaba 13416-000, SP, BrazilGroup of Alternative Analytical Approaches, Bioenergy Research Institute, Institute of Chemistry, São Paulo State University, Araraquara 14800-060, SP, BrazilGroup of Analytical Instrumental Analysis, Chemistry Department, Federal University of São Carlos, São Carlos 13565-905, SP, BrazilDepartment of Crop Science, School of Agricultural Sciences, São Paulo State University, Botucatu 18610-034, SP, BrazilPeanut seeds are harvested at different development stages (early and late) due to their uneven maturation. At the time of harvest, approximately 30% of the seeds are still immature, meaning they are not completely filled with compounds (e.g., oil and minerals) and exhibit reduced vigor. Hypothetically, these compounds can be detected as a “chemical fingerprinting” to classify seed maturation stages. Here, we investigated whether non-destructive techniques such as benchtop nuclear magnetic resonance (NMR), laser-induced breakdown spectroscopy (LIBS), and energy-dispersive X-ray fluorescence (ED-XRF) can identify chemical patterns unique to mature seeds with superior vigor. Field-grown seeds were classified into early (R5 and R6) and late (R7, R8, and R9) stages. Seed weight, germination, vigor, H<sub>2</sub>O<sub>2</sub>, and MDA (oxidative stress) were analyzed. Oil, potassium (K), and calcium (Ca) were measured digitally using spectroscopy techniques. We found that: (i) oxidative stress and K levels were higher in seeds from the early stages; (ii) seed oil and Ca were proportional to high-vigor seedlings and successful plant establishment in the field; and (iii) the seed chemical composition could be identified autonomously with 87% to 100% accuracy. In conclusion, LIBS, ED-XRF, and NMR technologies can effectively screen peanut seeds with superior vigor through “chemical fingerprinting”.https://www.mdpi.com/2073-4395/14/11/2529<i>Arachis hypogaea</i> L.NMRLIBSED-XRFCaK |
| spellingShingle | Gustavo Roberto Fonseca de Oliveira Welinton Yoshio Hirai Dennis Silva Ferreira Karolyne Priscila Oliveira Mota da Silva Giovani Chaves Silva Tiago Bueno Moraes Clissia Barboza Mastrangelo Fabiola Manhas Verbi Pereira Edenir Rodrigues Pereira-Filho Edvaldo Aparecido Amaral da Silva Spectroscopy Technologies to Screen Peanut Seeds with Superior Vigor Through “Chemical Fingerprinting” Agronomy <i>Arachis hypogaea</i> L. NMR LIBS ED-XRF Ca K |
| title | Spectroscopy Technologies to Screen Peanut Seeds with Superior Vigor Through “Chemical Fingerprinting” |
| title_full | Spectroscopy Technologies to Screen Peanut Seeds with Superior Vigor Through “Chemical Fingerprinting” |
| title_fullStr | Spectroscopy Technologies to Screen Peanut Seeds with Superior Vigor Through “Chemical Fingerprinting” |
| title_full_unstemmed | Spectroscopy Technologies to Screen Peanut Seeds with Superior Vigor Through “Chemical Fingerprinting” |
| title_short | Spectroscopy Technologies to Screen Peanut Seeds with Superior Vigor Through “Chemical Fingerprinting” |
| title_sort | spectroscopy technologies to screen peanut seeds with superior vigor through chemical fingerprinting |
| topic | <i>Arachis hypogaea</i> L. NMR LIBS ED-XRF Ca K |
| url | https://www.mdpi.com/2073-4395/14/11/2529 |
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